AB288183

Human DC-SIGNR ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Human DC-SIGNR ELISA Kit is a single-wash 90-min Simplestep used to quantify Human DC-SIGNR with a sensitivity of 3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD299, CD209L, CD209L1, CLEC4M, C-type lectin domain family 4 member M, CD209 antigen-like protein 1, DC-SIGN-related protein, Dendritic cell-specific ICAM-3-grabbing non-integrin 2, Liver/lymph node-specific ICAM-3-grabbing non-integrin, DC-SIGNR, DC-SIGN2, L-SIGN

3 Images

Sandwich ELISA - Human DC-SIGNR ELISA Kit (AB288183)

sELISA

Supplier Data

Sandwich ELISA - Human DC-SIGNR ELISA Kit (AB288183)

Serum of five individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean DC-SIGNR concentration was determined to be 871 pg/mL with a range of 450 - 1575 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human DC-SIGNR ELISA Kit (AB288183)

Interpolated concentration of native DC-SIGNR was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 20% and plasma-heparin 40%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human DC-SIGNR ELISA Kit (AB288183)

Example of human DC-SIGNR standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3 pg/mL

Range

23.438 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human DC-SIGNR ELISA Kit (ab288183) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DC-SIGNR protein in heparin plasma and serum samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human DC-SIGNR with 3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "98 - 106 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "84 - 123 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "112 - 120 %", "average": "= 95" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab288183-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB288183-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human DC-SIGNR Capture Antibody", "number":"AB288183-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human DC-SIGNR Lyophilized Recombinant Protein", "number":"AB288183-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB288183-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB288183-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB288183-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB288183-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB288183-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB288183-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human DC-SIGNR Detector Antibody", "number":"AB288183-CMP07", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

style

left-column

Product protocols

style

left-column

Target data

Probable pathogen-recognition receptor involved in peripheral immune surveillance in liver. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates.. (Microbial infection) Acts as an attachment receptor for Ebolavirus.. (Microbial infection) Acts as an attachment receptor for Hepatitis C virus.. (Microbial infection) Acts as an attachment receptor for HIV-1.. (Microbial infection) Acts as an attachment receptor for Human coronavirus 229E.. (Microbial infection) Acts as an attachment receptor for Human cytomegalovirus/HHV-5.. (Microbial infection) Acts as an attachment receptor for Influenzavirus.. (Microbial infection) Acts as an attachment receptor for SARS-CoV.. (Microbial infection) Acts as an attachment receptor for West-nile virus.. (Microbial infection) Acts as an attachment receptor for Japanese encephalitis virus.. (Microbial infection) Acts as an attachment receptor for Marburg virus glycoprotein.. (Microbial infection) Recognition of M.bovis by dendritic cells may occur partially via this molecule.

See full target information CLEC4M

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human DC-SIGNR ELISA Kit

Sandwich ELISA - Human DC-SIGNR ELISA Kit (AB288183)

Sandwich ELISA - Human DC-SIGNR ELISA Kit (AB288183)

Sandwich ELISA - Human DC-SIGNR ELISA Kit (AB288183)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Proteins & Peptides

Proteins & Peptides

Proteins & Peptides

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Product promise