Human DC-SIGNR ELISA Kit is a single-wash 90-min Simplestep used to quantify Human DC-SIGNR with a sensitivity of 3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 23.438 - 1500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Probable pathogen-recognition receptor involved in peripheral immune surveillance in liver. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates. (Microbial infection) Acts as an attachment receptor for Ebolavirus. (Microbial infection) Acts as an attachment receptor for Hepatitis C virus. (Microbial infection) Acts as an attachment receptor for HIV-1. (Microbial infection) Acts as an attachment receptor for Human coronavirus 229E. (Microbial infection) Acts as an attachment receptor for Human cytomegalovirus/HHV-5. (Microbial infection) Acts as an attachment receptor for Influenzavirus. (Microbial infection) Acts as an attachment receptor for SARS-CoV. (Microbial infection) Acts as an attachment receptor for West-nile virus. (Microbial infection) Acts as an attachment receptor for Japanese encephalitis virus. (Microbial infection) Acts as an attachment receptor for Marburg virus glycoprotein. (Microbial infection) Recognition of M.bovis by dendritic cells may occur partially via this molecule.
Alternative names
CD299, CD209L, CD209L1, CLEC4M, C-type lectin domain family 4 member M, CD209 antigen-like protein 1, DC-SIGN-related protein, Dendritic cell-specific ICAM-3-grabbing non-integrin 2, Liver/lymph node-specific ICAM-3-grabbing non-integrin, DC-SIGNR, DC-SIGN2, L-SIGN
What's included?
Recommended products
Human DC-SIGNR ELISA Kit is a single-wash 90-min Simplestep used to quantify Human DC-SIGNR with a sensitivity of 3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 23.438 - 1500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 3 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 24 | mean - | SD - | C.V. 5.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 6.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 102 | Range 98 - 106 % |
Sample type Heparin Plasma | Average % = 100 | Range 84 - 123 % |
Sample type Citrate plasma | Average % = 95 | Range 112 - 120 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human DC-SIGNR ELISA Kit (ab288183) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DC-SIGNR protein in heparin plasma and serum samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human DC-SIGNR with 3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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3 product images
Sandwich ELISA - Human DC-SIGNR ELISA Kit (ab288183)
Example of human DC-SIGNR standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human DC-SIGNR ELISA Kit (ab288183)
Serum of five individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean DC-SIGNR concentration was determined to be 871 pg/mL with a range of 450 - 1575 pg/mL.
Sandwich ELISA - Human DC-SIGNR ELISA Kit (ab288183)
Interpolated concentration of native DC-SIGNR was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 20% and plasma-heparin 40%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Product protocols
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