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AB242244

Human DcR3 ELISA Kit

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(1 Publication)

Human DcR3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human DcR3 with a sensitivity of 42 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

DCR3, TR6, UNQ186/PRO212, TNFRSF6B, Tumor necrosis factor receptor superfamily member 6B, Decoy receptor 3, Decoy receptor for Fas ligand, M68, DcR3

2 Images
Sandwich ELISA - Human DcR3 ELISA Kit (AB242244)
  • sELISA

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Sandwich ELISA - Human DcR3 ELISA Kit (AB242244)

Example of human DcR3 standard curve in Sample Diluent NS. The DcR3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human DcR3 ELISA Kit (AB242244)
  • sELISA

Supplier Data

Sandwich ELISA - Human DcR3 ELISA Kit (AB242244)

Interpolated concentrations of spiked DcR3 in human plasma (EDTA) and serum samples. The concentrations of DcR3 were measured in duplicates, interpolated from the DcR3 standard curves and corrected for sample dilution. Undiluted samples are as follows : plasma (EDTA) 100% The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 42 pg/mL

Range

63 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human DcR3 ELISA Kit (ab242244) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DcR3 protein in edta plasma and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human DcR3 with 42 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.5" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" } ]
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Recovery

[ { "sample": "Serum", "range": "82 - 97 %", "average": "= 87" }, { "sample": "EDTA Plasma", "range": "108 - 115 %", "average": "= 112" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab242244-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB242244-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB242244-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB242244-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB242244-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human DcR3 Lyophilized recombinant Protein", "number":"AB242244-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human DcR3 Capture Antibody", "number":"AB242244-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB242244-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human DcR3 Detector Antibody", "number":"AB242244-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB242244-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB242244-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Decoy receptor that can neutralize the cytotoxic ligands TNFS14/LIGHT, TNFSF15 and TNFSF6/FASL. Protects against apoptosis.
See full target information TNFRSF6B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 17:1057-1064 PubMed40092078

2025

Elevated Serum DcR3, Fas, and NLR Ratio are correlated with the progression of Liver Cirrhosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijun Lin,Yanchao Chen,Jiangying Long,Xianhua Zhang
View all publications
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websiteProtocolBooklet
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Product promise

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