AB309390
Human Dectin-1 ELISA Kit is a Sandwich ELISA for the measurement of Human Dectin-1 in Human in Cell Culture Media, Biofluids samples.
View Alternative Names
CD369, BGR, CLECSF12, DECTIN1, UNQ539/PRO1082, CLEC7A, C-type lectin domain family 7 member A, Beta-glucan receptor, C-type lectin superfamily member 12, Dendritic cell-associated C-type lectin 1, DC-associated C-type lectin 1, Dectin-1
1 Images
- sELISA
Supplier Data
Sandwich ELISA - Human Dectin-1 ELISA Kit (AB309390)
Example of standard curve using ab309390. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
Complementary Products
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
Product details
Human Dectin-1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Dectin-1 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Dectin-1 coated on a 96-well plate.
Recovery
[ { "sample": "Plasma", "range": "93 - 147 %", "average": "= 116.1" }, { "sample": "Serum", "range": "109 - 124 %", "average": "= 116.9" }, { "sample": "Cell culture media", "range": "94 - 140 %", "average": "= 113.3" } ]
What's included?
{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309390-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309390-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin", "number":"AB309390-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Dectin-1 Detection Antibody", "number":"AB309390-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309390-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Dectin-1 Protein Standard", "number":"AB309390-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B (Item E)", "number":"AB309390-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Dectin-1 Antibody-coated ELISA Plate", "number":"AB309390-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309390-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D (Item K)", "number":"AB309390-CMP06", "productcode":"" } ] } } }
Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Lectin that functions as a pattern recognizing receptor (PRR) specific for beta-1,3-linked and beta-1,6-linked glucans, which constitute cell wall constituents from pathogenic bacteria and fungi (PubMed : 11567029, PubMed : 12423684). Necessary for the TLR2-mediated inflammatory response and activation of NF-kappa-B : upon beta-glucan binding, recruits SYK via its ITAM motif and promotes a signaling cascade that activates some CARD domain-BCL10-MALT1 (CBM) signalosomes, leading to the activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (By similarity). Enhances cytokine production in macrophages and dendritic cells (By similarity). Mediates production of reactive oxygen species in the cell (By similarity). Mediates phagocytosis of C.albicans conidia (PubMed : 17230442). Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. Induces phosphorylation of SCIMP after binding beta-glucans (By similarity).
Product promise
We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com