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AB313506

Human DEGA ELISA Kit

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Human DEGA ELISA Kit is a Sandwich ELISA for the measurement of Human DEGA in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

ALI1, AMIGO2, Amphoterin-induced protein 2, AMIGO-2, Alivin-1, Differentially expressed in gastric adenocarcinomas, DEGA

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Sandwich ELISA - Human DEGA ELISA Kit (AB313506)
  • sELISA

Supplier Data

Sandwich ELISA - Human DEGA ELISA Kit (AB313506)

Example of standard curve using ab313506. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.2 ng/mL

Range

0.2 - 50 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB84416

Anti-DEGA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEGA antibody (AB84416)

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  • 2
Proteins & Peptides

AB276581

Recombinant Human DEGA protein (His tag)

SDS-PAGE - Recombinant Human DEGA protein (His tag) (AB276581)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human DEGA ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human DEGA in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human DEGA coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Arginase 1, B7-H4, Bcl-10, CD42b, CD73, CES1, CES2, cIAP-1, Cyclophilin A, Cystatin S, DNMT3A, Epimorphin, GDF-9, Glypican 3, GPR115, HE4, HO-1, HS3ST4, IGSF3, IL-17 RD, Integrin alpha 1, KIR2DL3, LAMP2, LEDGF, MOG, Nestin, Neudesin, Neuroligin 2, NKp80, Osteoadherin, PDGF R alpha, PRDX4, Syntaxin 4, TAFA1, TAFA2, TAFA5, Tenascin R, TGM4, TMEFF1.

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Recovery

[ { "sample": "Cell culture media", "range": "82 - 118 %", "average": "= 98.45" }, { "sample": "Plasma", "range": "76 - 94 %", "average": "= 82.89" }, { "sample": "Serum", "range": "81 - 104 %", "average": "= 95.66" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313506-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313506-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313506-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB313506-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human DEGA Antibody-coated ELISA Plate", "number":"AB313506-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313506-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human DEGA Detection Antibody", "number":"AB313506-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313506-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human DEGA Protein Standard", "number":"AB313506-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DEGA protein also known as DEGA-1 functions mechanically as a transmembrane protein involved in cellular adhesion. It has a mass of approximately 55 kDa and expresses widely in epithelial tissues where cellular interactions requiring adhesion are important for tissue integrity. DEGA is present in various cell types indicating its broad physiological relevance across tissues.
Biological function summary

This protein plays a role in modulating cell adhesion dynamics and contributes to tissue organization and stability. It forms part of adhesion complexes at the cellular surface interacting with cytoskeletal elements. Through these complexes DEGA helps maintain mechanical stability and signaling within epithelial cells making it an important component in tissue maintenance and repair.

Pathways

DEGA participates in cellular adhesion and signaling pathways essential for maintaining epithelial integrity. It interacts with proteins such as E-cadherin a well-known adhesion molecule forming a network that reinforces adhesion. This protein also integrates signals for pathway regulation playing a critical role in maintaining homeostasis in the epithelial layer.

DEGA is linked to epithelial-related conditions such as carcinomas where altered adhesion contributes to disease progression. It interacts with proteins involved in tumorigenesis such as E-cadherin and beta-catenin. The loss or dysfunction of DEGA can lead to disrupted cell adhesion contributing to the invasive characteristics of cancerous cells highlighting its importance in disease pathology.
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Product protocols

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Target data

Required for depolarization-dependent survival of cultured cerebellar granule neurons. May mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. May contribute to signal transduction through its intracellular domain. May be required for tumorigenesis of a subset of gastric adenocarcinomas.
See full target information AMIGO2
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websiteProtocolBooklet
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Product promise

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