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AB108728

Human Anti-Dengue virus IgG ELISA Kit

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(3 Publications)

Human Anti-Dengue virus IgG ELISA Kit is an indirect ELISA for the qualitative detection of IgG class antibodies against Dengue virus in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Anti-Dengue virus IgG ELISA Kit is an indirect ELISA for the qualitative detection of IgG class antibodies against Dengue virus in human plasma and serum samples.

How the assay works:

A 96-well plate has been precoated with Dengue virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Dengue virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Dengue virus IgG sample captured in plate.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Anti-Dengue virus IgG ELISA Kit ab108728 protocol summary

1. Add standard or sample to appropriate wells. Incubate at 37°C
2. Wash each well and add prepared labeled HRP-Conjugate. Incubate at room temperature
3. After washing, add TMB substrate solution to each well. Incubate at room temperature
4. Add Stop Solution to each well. Read immediately.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.76" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.34" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108728-1x96Tests", "assetComponentDetails": [ { "size":"1 x 2 mL", "name":"Dengue virus IgG Positive Control", "number":"AB108728-CMP06", "productcode":"" }, { "size":"1 x 2 mL", "name":"Dengue virus IgG Negative Control", "number":"AB108728-CMP08", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgG Sample Diluent", "number":"AB108728-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Dengue virus (IgG) Coated Microplate (12 x 8 wells)", "number":"AB108728-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB108728-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"Dengue virus anti-IgG HRP Conjugate", "number":"AB108728-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108728-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108728-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108728-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108728-CMP09", "productcode":"" }, { "size":"1 x 3 mL", "name":"Dengue virus IgG Cut-off Control", "number":"AB108728-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dengue Virus IgG also known as anti-dengue IgG is an immunoglobulin G antibody subtype that targets the dengue virus a member of the Flaviviridae family. It has a molecular mass of approximately 150 kDa and expresses significantly in individuals who have been infected with the dengue virus. This antibody plays an essential role in the body's immune response to dengue infection. Dengue Virus IgG is an important marker in serological assays including the ELISA test for dengue which helps detect past dengue infections. ELISA kit development often includes these specific antibodies to ensure high sensitivity and specificity.
Biological function summary

Dengue-specific IgG antibodies contribute to the immune system's ability to recognize and neutralize the dengue virus. These antibodies form complexes with viral antigens which help facilitate the clearance of the virus from the bloodstream. The presence and concentration of dengue IgG in serum indicate an ongoing or past infection. IgG antibodies play a role in secondary immune responses providing long-term immunity and protection against subsequent dengue virus exposures to some extent. The immune system relies on the production of these antibodies to maintain a state of readiness against the dengue virus.

Pathways

Dengue-specific IgG antibodies participate in various immune pathways particularly linking to the humoral immunity and Fc-gamma receptor-mediated pathways. These pathways are important in opsonization a process where antibodies bind to a pathogen to mark it for destruction by phagocytes. Additionally interactions with other proteins such as complement components and immunoglobulin receptors highlight the complex nature of IgG's involvement in protective immune responses. These connections emphasize the role of antibodies in coordinating and executing an effective immune response against dengue virus infection.

Dengue-specific IgG antibodies are significantly involved in the diagnosis and understanding of dengue fever and dengue hemorrhagic fever. Dengue fever is a mosquito-borne viral disease that can range from mild to severe causing symptoms such as high fever rash and muscle pain. In patients with a history of previous dengue infection the presence of dengue IgG can lead to an enhanced immune response sometimes resulting in more severe forms of the disease such as dengue hemorrhagic fever due to a phenomenon called antibody-dependent enhancement (ADE). This links the dengue IgG with viral proteins such as the non-structural protein 1 (NS1) which plays a role in the severity and progression of these diseases.
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Product protocols

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Target data

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 12:703887 PubMed34367162

2021

Adapting Rapid Diagnostic Tests to Detect Historical Dengue Virus Infections.

Applications

Unspecified application

Species

Unspecified reactive species

Fernando Echegaray,Peter Laing,Samantha Hernandez,Sully Marquez,Amanda Harris,Ian Laing,Adam Chambers,Neil McLennan,Victor A Sugiharto,Hua-Wei Chen,Sandra Vivero Villagran,Abigail Collingwood,Magelda Montoya,Fausto Bustos Carrillo,Mark P Simons,Philip J Cooper,Andrea Lopez,Gabriel Trueba,Joseph Eisenberg,Shuenn-Jue Wu,William Messer,Eva Harris,Josefina Coloma,Leah C Katzelnick

EBioMedicine 48:425-441 PubMed31526718

2019

High levels of serum hyaluronan is an early predictor of dengue warning signs and perturbs vascular integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yu Lin,Constantinos Kolliopoulos,Chung-Hao Huang,Jyrki Tenhunen,Carl-Henrik Heldin,Yen-Hsu Chen,Paraskevi Heldin

Methods in molecular biology (Clifton, N.J.) 1604:55-78 PubMed28986825

2017

Epidemiological Surveillance of Viral Hemorrhagic Fevers With Emphasis on Clinical Virology.

Applications

ELISA

Species

Human

Carolina Montoya-Ruiz,Juan David Rodas
View all publications
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websiteProtocolBooklet
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