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AB108729

Human Anti-Dengue virus IgM ELISA Kit

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(3 Publications)

Human Anti-Dengue virus IgM ELISA Kit is an indirect ELISA for the qualitative detection of IgM class antibodies against Dengue virus in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Dengue virus ELISA kit - IgM is designed for the accurate qualitative measurement of IgM class antibodies against Dengue virus in Human serum and plasma.

A 96-well plate has been precoated with Dengue virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Dengue virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Dengue virus IgM sample captured in plate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 20, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 7, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108729-1x96Tests", "assetComponentDetails": [ { "size":"1 x 2 mL", "name":"Dengue virus IgM Positive Control", "number":"AB108729-CMP06", "productcode":"" }, { "size":"1 x 3 mL", "name":"Dengue virus IgM Cut-off Control", "number":"AB108729-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108729-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB108729-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Dengue virus (IgM) Coated Microplate (12 x 8 wells)", "number":"AB108729-CMP05", "productcode":"" }, { "size":"1 x 2 mL", "name":"Dengue virus IgM Negative Control", "number":"AB108729-CMP08", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgM Sample Diluent", "number":"AB108729-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108729-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108729-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108729-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"Dengue virus anti-IgM HRP Conjugate", "number":"AB108729-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dengue Virus IgM also known as anti-Dengue IgM is an antibody target involved in the immune response against the Dengue virus. This immunoglobulin M (IgM) is produced soon after Dengue virus infection. While the exact mass of Dengue Virus IgM is not specific to a single value typical IgM has a mass of approximately 950 kDa. Dengue Virus IgM can be detected in human serum or plasma indicating an acute phase of infection. It's not a protein but an antibody produced by B cells when the immune system encounters the Dengue virus.
Biological function summary

Anti-Dengue IgM antibodies play a role in the early immune response to Dengue infection. They form part of the humoral immune system and contribute to pathogen neutralization. These antibodies can exist as pentameric complexes that enhance their ability to bind to the virus. Once produced they help prevent the virus from entering host cells and assist other components of the immune system in identifying and eliminating the virus. Their presence indicates an active or recent Dengue virus infection making them important in Dengue serology.

Pathways

Dengue Virus IgM is part of the immune pathway responding to viral infection. Such pathways include the complement activation pathway where IgM antibodies activate the complement system to eliminate pathogens efficiently. Additionally they can interact with Fc receptors on immune cells enhancing phagocytosis of the virus. Through these interactions Dengue Virus IgM aids in the broader adaptive immune response involving helper T cells and other immunoglobulins such as IgG.

Dengue Virus IgM is directly associated with Dengue fever an acute viral illness spread by mosquitoes. The presence of Dengue Virus IgM in a host is a clinical indicator of Dengue virus infection and plays an important role in diagnostic assessments using methods such as IgM capture ELISA or Dengue ELISA kits. This antibody can contribute to immune-mediated reactions such as immune enhancement where the presence of different non-neutralizing antibodies may facilitate severe illness. IgM aids in differentiating between primary and secondary Dengue infections which differ in severity and immune response potentially influencing the risk of developing more serious conditions such as Dengue hemorrhagic fever.
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Product protocols

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Target data

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 48:425-441 PubMed31526718

2019

High levels of serum hyaluronan is an early predictor of dengue warning signs and perturbs vascular integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yu Lin,Constantinos Kolliopoulos,Chung-Hao Huang,Jyrki Tenhunen,Carl-Henrik Heldin,Yen-Hsu Chen,Paraskevi Heldin

International journal of infectious diseases : IJI 84S:S57-S63 PubMed30658170

2019

Analysis of dengue specific memory B cells, neutralizing antibodies and binding antibodies in healthy adults from India.

Applications

ELISA

Species

Human

Sivaram Gunisetty,Kaustuv Nayak,Ramesh Chandra Rai,Yadya Chawla,Elluri Seetharami Reddy,Charu Aggarwal,Deepti Maheshwari,Harekrushna Panda,Nasim Akhtar Ansari,Prabhat Singh,Manpreet Kaur,Kritika Dixit,Pragati Sharma,Priya Bhatnagar,Lalita Priyamvada,Siddhartha Kumar Bhaumik,Syed Fazil Ahamed,Rosario Vivek,Pratima Ray,Anita Shet,Poonam Coshic,Rakesh Lodha,Sushil Kumar Kabra,Dil Afroze,Adfar Yousuf,Rafi Ahmed,Kaja Murali-Krishna,Anmol Chandele

Methods in molecular biology (Clifton, N.J.) 1604:55-78 PubMed28986825

2017

Epidemiological Surveillance of Viral Hemorrhagic Fevers With Emphasis on Clinical Virology.

Applications

ELISA

Species

Human

Carolina Montoya-Ruiz,Juan David Rodas
View all publications
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