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AB309442

Human Desmocollin 2 ELISA kit

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Human Desmocollin 2 ELISA kit is a Sandwich (quantitative) ELISA for the measurement of Human Desmocollin 2 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CDHF2, DSC3, DSC2, Desmocollin-2, Cadherin family member 2, Desmocollin-3, Desmosomal glycoprotein II, Desmosomal glycoprotein III

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Sandwich ELISA - Human Desmocollin 2 ELISA kit (AB309442)
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Supplier Data

Sandwich ELISA - Human Desmocollin 2 ELISA kit (AB309442)

Example of standard curve using ab309442. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 21 pg/mL

Range

21 - 5000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Desmocollin 2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Desmocollin 2 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human desmocollin 2 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "82 - 117 %", "average": "= 99.08" }, { "sample": "Plasma", "range": "83 - 134 %", "average": "= 109.3" }, { "sample": "Cell culture supernatant", "range": "70 - 80 %", "average": "= 73.96" }, { "sample": "Cell culture media", "range": "70 - 80 %", "average": "= 73.96" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309442-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309442-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin", "number":"AB309442-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Desmocollin-2 Protein Standard", "number":"AB309442-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309442-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309442-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Desmocollin-2 Detection Antibody", "number":"AB309442-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309442-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Desmocollin-2 Antibody-coated ELISA Plate", "number":"AB309442-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Desmocollin 2 also known as DSC2 is a cadherin protein and an important component of desmosomes which are structures that facilitate cell-cell adhesion in epithelial tissues. It has a molecular weight of approximately 100 kDa. This protein expresses strongly in tissues rich in desmosomes such as the epidermis and cardiac tissue. Desmocollin 2 stabilizes cells against shearing forces ensuring that they adhere tightly by interacting with other desmosomal proteins.
Biological function summary

Desmocollin 2 plays a role in maintaining tissue integrity and cohesion. It participates as a part of the desmosomal complex where it interacts with other cadherin family members like desmogleins and anchors to the intermediate filaments inside the cell. These interactions help to maintain the architecture and structural support during mechanical stress especially in tissues undergoing constant stress like the heart and skin.

Pathways

Desmocollin 2's mechanical role intersects with various cell signaling pathways. Notably it plays a role in the Wnt signaling pathway which is important for cell proliferation and differentiation. The protein also interacts with plakoglobin a protein involved in beta-catenin regulation within these pathways. Desmocollin 2's involvement in cell adhesion and signaling nodes suggests its contribution to further complex signaling cascades.

Desmocollin 2 mutations and disruptions associate with arrhythmogenic right ventricular cardiomyopathy (ARVC) and certain skin blistering conditions. In ARVC the weakened cell adhesion due to desmocollin 2 defects can lead to fibrofatty replacement of myocardium impacting cardiac function. The protein plakoglobin often appears as a related factor in these diseases as both proteins interact at the desmosomal junctions and contribute to cell adhesion integrity.
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Product protocols

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Target data

Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms. May promote p38MAPK signaling activation that facilitates keratinocyte migration (By similarity).
See full target information DSC2
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Complementary Products

Primary Antibodies

AB95967

Anti-Desmocollin 2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmocollin 2 antibody (AB95967)

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