JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB309411

Human Desmocollin 3 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Desmocollin 3 ELISA Kit is a Sandwich ELISA for the measurement of Human Desmocollin 3 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CDHF3, DSC4, DSC3, Desmocollin-3, Cadherin family member 3, Desmocollin-4, HT-CP

1 Images
Sandwich ELISA - Human Desmocollin 3 ELISA Kit (AB309411)
  • sELISA

Supplier Data

Sandwich ELISA - Human Desmocollin 3 ELISA Kit (AB309411)

Example of standard curve using ab309411. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.16 ng/mL

Range

0.16 - 40 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Primary Antibodies

AB126768

Anti-Desmocollin 3 antibody [EPR7486]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Desmocollin 3 antibody [EPR7486] (AB126768)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Desmocollin 3 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD42b in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Desmocollin 3 coated on a 96-well plate.
style
left-column

Recovery

[ { "sample": "Serum", "range": "88 - 132 %", "average": "= 117.2" }, { "sample": "Plasma", "range": "80 - 120 %", "average": "= 99.29" }, { "sample": "Cell culture supernatant", "range": "94 - 138 %", "average": "= 118.1" }, { "sample": "Cell culture media", "range": "94 - 138 %", "average": "= 118.1" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309411-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309411-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human DSC-3 Antibody-coated ELISA Plate", "number":"AB309411-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"500X HRP-Streptavidin", "number":"AB309411-CMP12", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309411-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309411-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human DSC-3 Detection Antibody", "number":"AB309411-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309411-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human DSC-3 Protein Standard", "number":"AB309411-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Desmocollin 3 also known as DSC3 is a desmosomal cadherin protein with a molecular mass of approximately 100 kDa. It plays an important role in cell adhesion by forming part of the desmosomes which are specialized junctions that connect adjacent cells. DSC3 is primarily expressed in epithelial tissues such as the skin and mucous membranes. By contributing to the structural integrity of epithelial cell layers DSC3 ensures cells stick together firmly providing mechanical stability.
Biological function summary

Desmocollin 3 is a part of the cadherin superfamily which are calcium-dependent adhesion proteins. Within desmosomes DSC3 interacts closely with desmogleins and other cadherins to form strong intercellular connections. This protein is an essential component of the desmosomal adhesion complex that helps maintain tissue architecture. The interaction between DSC3 and other cadherins regulates the cohesion and communication between epithelial cells which is vital for normal tissue function.

Pathways

Desmocollin 3 is an important participant in the cadherin signaling pathway and intercellular junction organization. These pathways are important for the regulation of tissue morphogenesis and the maintenance of cell polarity and tissue architecture. DSC3 functions alongside proteins like plakoglobin an integral part of the desmosome complex that helps bridge cadherins with cytoskeletal elements. This intricate network supports tissue resilience and adaptive responses to mechanical stress.

Desmocollin 3 has strong associations with skin diseases such as pemphigus vulgaris and squamous cell carcinoma. In pemphigus vulgaris antibodies target desmosomal components like DSC3 leading to a loss of cell adhesion and blistering of the skin. In squamous cell carcinoma aberrant expression or function of DSC3 can contribute to the invasive potential of cancer cells. DSC3's interaction with desmogleins in these conditions highlights its importance in disease mechanisms and potential as a therapeutic target.
style
left-column

Product protocols

style
left-column

Target data

A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (By similarity). Required for cell-cell adhesion in the epidermis, as a result required for the maintenance of the dermal cohesion and the dermal barrier function (PubMed : 19717567). Required for cell-cell adhesion of epithelial cell layers surrounding the telogen hair club, as a result plays an important role in telogen hair shaft anchorage (By similarity). Essential for successful completion of embryo compaction and embryo development (By similarity).
See full target information DSC3
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com