Human Desmoglein 2/DSG2 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Desmoglein 2/DSG2 in Human in Serum, Plasma, Cell culture supernatant samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 16 - 4000 pg/mL
- Sensitivity
- = 16 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
Alternative names
CDHF5, DSG2, Desmoglein-2, Cadherin family member 5, HDGC
What's included?
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Human Desmoglein 2/DSG2 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Desmoglein 2/DSG2 in Human in Serum, Plasma, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 16 - 4000 pg/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 16 pg/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 123.8 | Range 107 - 143 % |
Sample type Plasma | Average % = 108.3 | Range 80 - 123 % |
Sample type Cell culture media | Average % = 80.08 | Range 68 - 90 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human Desmoglein 2/DSG2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Desmoglein 2/DSG2 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Desmoglein 2/DSG2 coated on a 96-well plate.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAM12, ADAM-8, B7-H3 (CD276), BMPR-IB, Cadherin-13, Cadherin-4, CD155 (PVR), CD229 (SLAMF3), CD48 (SLAMF2), CD58 (LFA-3), CD84 (SLAMF5), CD99, CEACAM-5, Coagulation Factor XIV (Protein C), Cystatin A, Cystatin B, Cystatin E/M, DR3 (TNFRSF25), ErbB4 (HER4), ESAM, FGF-21, Galectin-2, Galectin-9, ICOS, JAM-A (CD321/F11R), JAM-B (VE-JAM/CD322), Kallikrein 5, Midkine, Pentraxin-3 (TSG-14), Pref-1 (DLK-1/FA1), Siglec10, SLAM (CD150), SP-D, Syndecan-4, Testican 2 (SPOCK2), TIM-3 (KIM-3), TLR4, TRAIL (TNFSF10), ULBP-1.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Desmoglein 2 (DSG2) is a member of the desmoglein family of proteins sometimes referred to as desmoglein-2 or DSG 2. This protein is a calcium-binding transmembrane glycoprotein and part of the cadherin family with a molecular mass of approximately 122 kDa. DSG2 plays a mechanical role in cell-cell adhesion by forming desmosomes which are specialized structures for maintaining the integrity of tissues. DSG2 is mainly expressed in epithelial cells including those found in the heart skin and certain internal organs.
- Biological function summary
DSG2 contributes to the structural stability of tissues by locking cells together forming a mechanical and signaling function. The protein is integral to the composition of desmosomes complexes that include other components such as plakoglobin and desmoplakin. These complexes strengthen intercellular adhesion which is essential for tissues that experience constant physical stress like the heart and skin. DSG2 actively participates in cell signaling impacting cellular behavior and communication.
- Pathways
DSG2 plays significant roles in the heart development and epidermal differentiation pathways. DSG2 interacts with several other proteins including plakoglobin and desmoplakin to maintain cellular adhesion and tissue morphogenesis. In the cardiac setting the heart development pathway connects DSG2 with other proteins essential for maintaining the integrity and signaling within the cardiac muscle. This interaction helps modulate pathways that ensure proper cardiac function and repair processes.
- Associated diseases and disorders
DSG2 is linked to arrhythmogenic right ventricular cardiomyopathy (ARVC) which affects the heart's rhythm and muscle structure. DSG2 mutations can disrupt desmosomal structure weakening cell adhesion and leading to disease. Another related disorder is pemphigus vulgaris an autoimmune disease where antibodies target DSG2 among other desmogleins disrupting cell adhesion in the skin and mucous membranes. In ARVC DSG2 frequently connects with other proteins like plakoglobin that are involved in desmosomal interactions leading to the structural and functional deficits seen in the disease.
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1 product image
Sandwich ELISA - Human Desmoglein 2/DSG2 ELISA Kit (ab314727)
Example of standard curve using ab314727. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
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Product protocols
- Visit the general protocols
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