Human Dipeptidyl Peptidase IV ELISA Kit (CD26)

Product details

Human Dipeptidyl Peptidase IV ELISA Kit (CD26) (ab222872) is designed for the quantitative measurement of Dipeptidyl Peptidase IV (DPP4) in human plasma, serum cell lysate, tissue and cell culture samples.

This assay employs a quantitative sandwich enzyme immunoassay technique that measures human DPP4 in less than 4 hours. A polyclonal antibody specific for human DPP4 has been pre-coated onto a 96-well microplate with removable strips. DPP4 in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for DPP4, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Dipeptidyl peptidase 4 (DPP4), also known as adenosine deaminase complexing protein 2 (ADCP-2), T-cell activation antigen CD26 or CD26, belongs to the peptidase S9B family and DPPIV subfamily. It is an intrinsic membrane glycoprotein that comprises 766 amino acids and weighs 110 kDa. Its extracellular cysteine-rich region is necessary for association with collagen, dimer formation and optimal dipeptidyl peptidase activity. Under certain stimuli and chronic inflammation, DPP4 can be released from the membrane, constituting a soluble form by matrix metalloproteases (MMPs). As a serine exopeptidase, DPP4 cleaves and inactivates N-terminal X-proline dipeptides of cytokines, chemokines, and neuropeptides involved in inflammation, immunity, and vascular function. Circulating soluble DPP4 is a novel adipokine that may impair insulin sensitivity in an autocrine and paracrine fashion. DPP4 plays a role in glucose homeostasis through proteolytic inactivation of the intestinal peptide incretins, such as glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide 1 (GLP1), which are major regulators of post-prandial insulin secretion.