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AB100501

Human DKK1 ELISA Kit (Dickkopf-1)

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(5 Publications)

Human DKK1 ELISA Kit (Dickkopf-1) is a Sandwich ELISA for the measurement of Human DKK1 (Dickkopf-1) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

UNQ492/PRO1008, DKK1, Dickkopf-related protein 1, Dickkopf-1, Dkk-1, hDkk-1, SK

2 Images
Sandwich ELISA - Human DKK1 ELISA Kit (Dickkopf-1) (AB100501)
  • sELISA

Supplier Data

Sandwich ELISA - Human DKK1 ELISA Kit (Dickkopf-1) (AB100501)

Representative Standard Curve using ab100501.

Sandwich ELISA - Human DKK1 ELISA Kit (Dickkopf-1) (AB100501)
  • sELISA

Supplier Data

Sandwich ELISA - Human DKK1 ELISA Kit (Dickkopf-1) (AB100501)

Representative Standard Curve using ab100501.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 100 pg/mL

Range

122.9 - 30000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's DKK1 (Dickkopf-1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human DKK1 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human DKK1 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human DKK1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "93 - 123 %", "average": "= 108.3" }, { "sample": "Serum", "range": "62 - 130 %", "average": "= 92.3" }, { "sample": "Plasma", "range": "59 - 110 %", "average": "= 72.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100501-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB100501-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100501-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"DKK1 Microplate (12 x 8 wells)", "number":"AB100501-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100501-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100501-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100501-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human DKK1", "number":"AB100501-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human DKK1 Standard (lyophilized)", "number":"AB100501-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"700X HRP-Streptavidin Concentrate", "number":"AB100501-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DKK1 protein also known as Dickkopf-1 is a secreted protein with a mass of approximately 28-30 kDa. It plays a mechanical role in inhibiting the Wnt signaling pathway by binding to LRP5/6 co-receptors preventing Wnt ligands from activating the pathway. DKK1 is expressed in various tissues including the bone brain and placenta reflecting its involvement in several biological processes. Its capacity to bind and neutralize the Wnt pathway components cements DKK1's role as a significant modulator of cellular functions.
Biological function summary

The inhibition of the Wnt signaling by DKK1 alters cell proliferation differentiation and migration. DKK1 does not function as part of a large multi-protein complex but interacts directly with specific co-receptors and modulates the activity of key signaling molecules. This regulatory mechanism controls processes like bone formation and neurogenesis indicating DKK1's broad impact in development and tissue homeostasis. Its expression pattern and biological role position it as an important player in maintaining proper cellular signaling environments.

Pathways

DKK1 is a critical player in the Wnt signaling and bone remodeling pathways. Through its interaction with Wnt pathway components like LRP5/6 DKK1 modulates these pathways affecting downstream proteins such as β-catenin and influencing gene transcription. In the bone remodeling context DKK1's ability to regulate osteoblast activity connects it to other related proteins like sclerostin contributing to bone density and health.

Elevated levels of DKK1 are observed in conditions like osteoporosis and cancer. In osteoporosis DKK1's inhibition of Wnt signaling leads to reduced bone formation implicating it alongside proteins like sclerostin in deteriorating bone health. In cancer DKK1's dysregulation can promote tumorigenesis by altering cell signaling and microenvironment interactions. These roles underline the potential of targeting DKK1 for therapeutic purposes in treating these diseases.
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Product protocols

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Target data

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed : 22000856). DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (PubMed : 17143291). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (By similarity).
See full target information DKK1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37834418

2023

Dickkopf-Related Protein 1 (DKK-1) as a Possible Link between Bone Erosions and Increased Carotid Intima-Media Thickness in Psoriatic Arthritis: An Ultrasound Study.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina-Elena Biță,Ștefan Cristian Dinescu,Anca-Lelia Riza,Paulina Lucia Ciurea,Anca Emanuela Mușetescu,Daniela Marinescu,Roxana Mihaela Dumitrașcu,Larisa Ionela Șuiu,Răzvan Adrian Ionescu,Horațiu Valeriu Popoviciu,Florentin Ananu Vreju

Journal of cellular physiology 237:551-565 PubMed34224151

2021

Lipocalin 2 increases after high-intensity exercise in humans and influences muscle gene expression and differentiation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Ponzetti,Federica Aielli,Argia Ucci,Alfredo Cappariello,Giovanni Lombardi,Anna Teti,Nadia Rucci

Scientific reports 11:9920 PubMed33972574

2021

Validation of a DKK1 RNAscope chromogenic in situ hybridization assay for gastric and gastroesophageal junction adenocarcinoma tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Charles Caldwell,James B Rottman,Will Paces,Elizabeth Bueche,Sofia Reitsma,Joseph Gibb,Vitria Adisetiyo,Michael S Haas,Heidi Heath,Walter Newman,Jason Baum,Roberto Gianani,Michael H Kagey

World journal of clinical cases 8:1213-1222 PubMed32337195

2020

Clinical significance and prognostic value of tumor necrosis factor-α and dickkopf related protein-1 in ankylosing spondylitis.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Hui Xiong,Jian Liu,Jian Chen

Journal of cellular physiology 234:12897-12909 PubMed30556904

2018

Priming integrin α5 promotes human dental pulp stem cells odontogenic differentiation due to extracellular matrix deposition and amplified extracellular matrix-receptor activity.

Applications

Unspecified application

Species

Unspecified reactive species

He Wang,Tingting Ning,Ci Song,Xinghong Luo,Shuaimei Xu,Xiaoyi Zhang,Zilong Deng,Dandan Ma,Buling Wu
View all publications
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