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AB236710

Human DKK3 ELISA Kit

5

(1 Review)

|

(1 Publication)

Human DKK3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human DKK3 with a sensitivity of 19.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

REIC, UNQ258/PRO295, DKK3, Dickkopf-related protein 3, Dickkopf-3, Dkk-3, hDkk-3

4 Images
Sandwich ELISA - Human DKK3 ELISA Kit (AB236710)
  • sELISA

Lab

Sandwich ELISA - Human DKK3 ELISA Kit (AB236710)

The DKK3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human DKK3 ELISA Kit (AB236710)
  • sELISA

Lab

Sandwich ELISA - Human DKK3 ELISA Kit (AB236710)

Interpolated concentrations of native DKK3 in human cell culture supernatant samples. The concentrations of DKK3 were measured in duplicates, interpolated from the DKK3 standard curves and corrected for sample dilution. Undiluted samples are as follows : HUVEC supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DKK3 concentration was determined to be 19.0 ng/mL in HUVEC supernatant.

Sandwich ELISA - Human DKK3 ELISA Kit (AB236710)
  • sELISA

Lab

Sandwich ELISA - Human DKK3 ELISA Kit (AB236710)

Interpolated concentrations of native DKK3 in human serum and plasma samples. The concentrations of DKK3 were measured in duplicates, interpolated from the DKK3 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 10%, plasma (heparin) 10%, and plasma (EDTA) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DKK3 concentration was determined to be 37.5 ng/mL in serum, 30.5 ng/mL in plasma (citrate), 29.6 ng/ml in plasma (heparin), and 29.0 ng/mL in plasma (EDTA).

Sandwich ELISA - Human DKK3 ELISA Kit (AB236710)
  • sELISA

Supplier Data

Sandwich ELISA - Human DKK3 ELISA Kit (AB236710)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DKK3 concentration was determined to be 40.4 ng/mL with a range of 27.3 – 65.8 ng/mL.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 19.3 pg/mL

Range

78.1 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human DKK3 ELISA Kit (ab236710) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DKK3 protein in cit plasma, edta plasma, hep plasma, serum, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human DKK3 with 34.1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" } ]
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Recovery

[ { "sample": "Serum", "range": "93 - 118 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "90 - 103 %", "average": "= 91" }, { "sample": "Heparin Plasma", "range": "91 - 107 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "97 - 109 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "84 - 96 %", "average": "= 91" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).
See full target information DKK3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinics (Sao Paulo, Brazil) 79:100360 PubMed38678874

2024

Predictive value of glycoprotein DKK3 for early neurological deterioration after ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

DongLiang Zhou,HongWei Qin,Lei Miao,Ying Xu,Lan Yu,JianMin Wang
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