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AB100502

Human DKK3 ELISA Kit (Dickkopf-3)

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(2 Publications)

Human DKK3 ELISA Kit (Dickkopf-3) is a Sandwich (quantitative) ELISA for the measurement of Human DKK3 (Dickkopf-3) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

REIC, UNQ258/PRO295, DKK3, Dickkopf-related protein 3, Dickkopf-3, Dkk-3, hDkk-3

2 Images
Sandwich ELISA - Human DKK3 ELISA Kit (Dickkopf-3) (AB100502)
  • sELISA

Supplier Data

Sandwich ELISA - Human DKK3 ELISA Kit (Dickkopf-3) (AB100502)

Representative Standard Curve using ab100502.

Sandwich ELISA - Human DKK3 ELISA Kit (Dickkopf-3) (AB100502)
  • sELISA

Supplier Data

Sandwich ELISA - Human DKK3 ELISA Kit (Dickkopf-3) (AB100502)

Representative Standard Curve using ab100502.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 3 pg/mL

Range

2.74 - 2000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's DKK3 (Dickkopf-3) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human DKK3 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human DKK3 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human DKK3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimisation may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "85 - 105 %", "average": "= 96.23" }, { "sample": "Serum", "range": "77 - 96 %", "average": "= 86.69" }, { "sample": "Plasma", "range": "80 - 98 %", "average": "= 89.24" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100502-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100502-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100502-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB100502-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100502-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human DKK3", "number":"AB100502-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human DKK3 Standard (lyophilized)", "number":"AB100502-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100502-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"DKK3 Microplate (12 x 8 well strips)", "number":"AB100502-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100502-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DKK3 also known as Dickkopf-related protein 3 or Dickkopf-3 is a member of the Dickkopf protein family. This secreted protein has a molecular mass of approximately 38 kDa. DKK3 is expressed in multiple tissues including heart brain and kidney. It plays a role in modulating signaling pathways by acting as a Wnt signaling pathway antagonist. It's important in the regulation of developmental processes and is often investigated in various cellular contexts.
Biological function summary

DKK3 functions as an inhibitor of the Wnt/beta-catenin signaling pathway. It is not known to be a part of a larger protein complex but likely interacts with specific receptors and co-receptors at the cell surface to modulate signaling events. This regulation influences cell fate decisions proliferation and differentiation making DKK3 an essential player in cellular development and homeostasis.

Pathways

DKK3 significantly impacts the Wnt and TGF-beta signaling pathways. As an inhibitor of Wnt signaling DKK3 prevents the stabilization and accumulation of beta-catenin in the cytoplasm thereby affecting gene transcription in the nucleus. DKK3's interaction with TGF-beta proteins also influences cellular responses to growth factors and cytokines facilitating communication within the cell and its environment.

Alterations in DKK3 expression have links to cancers and cardiovascular diseases. In cancer DKK3 often exhibits reduced expression affecting pathways that regulate tumor growth and metastasis. In cardiovascular disorders DKK3 interacts with proteins such as Frizzled and LRP5/6 which relate to disease pathogenesis. Monitoring DKK3 using assays like DKK3 ELISA can help in understanding its role in disease progression and could serve as a potential biomarker or therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).
See full target information DKK3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biology 9: PubMed33023021

2020

Circulating Biomarkers of Accelerated Sarcopenia in Respiratory Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Rizwan Qaisar,Asima Karim,Tahir Muhammad,Islam Shah

Journal of dermatological science 84:88-96 PubMed27354306

2016

The expression pattern and functional role of REIC/Dkk-3 in the development of cutaneous squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jung-Min Shin,Dae-Kyoung Choi,Hye-Young Kang,Kyung-Cheol Sohn,Young Lee,Chang Deok Kim,Jeung-Hoon Lee,Byung Cheol Park
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

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