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AB100503

Human DKK4 ELISA Kit (Dickkopf-4)

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Human DKK4 ELISA Kit (Dickkopf-4) is a Sandwich (quantitative) ELISA for the measurement of Human DKK4 (Dickkopf-4) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Dickkopf-related protein 4, Dickkopf-4, Dkk-4, hDkk-4, DKK4

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Sandwich ELISA - Human DKK4 ELISA Kit (Dickkopf-4) (AB100503)
  • sELISA

Supplier Data

Sandwich ELISA - Human DKK4 ELISA Kit (Dickkopf-4) (AB100503)

Representative Standard Curve using ab100503.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

13.72 - 10000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's DKK4 (Dickkopf-4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human DKK4 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human DKK4 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human DKK4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimisation may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "97 - 121 %", "average": "= 109.6" }, { "sample": "Serum", "range": "85 - 105 %", "average": "= 95.59" }, { "sample": "Plasma", "range": "69 - 91 %", "average": "= 82.62" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100503-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100503-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100503-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100503-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"DKK4 Microplate (12 x 8 well strips)", "number":"AB100503-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB100503-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100503-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human DKK4 Standard (lyophilized)", "number":"AB100503-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human DKK4", "number":"AB100503-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dickkopf-related protein 4 (DKK4) acts as an inhibitor of the Wnt signaling pathway which affects cell fate proliferation and migration. Known aliases for DKK4 include DKK-4 and DKK-4 protein. It possesses a molecular mass of around 27 kDa. DKK4 mainly expresses in the skin gastrointestinal tract and placenta indicating roles in these tissues and systems. Its presence suggests involvement in processes related to epithelial and stromal interactions.
Biological function summary

DKK4 participates in the regulation of embryonic development and tissue homeostasis. It functions as a part of the larger Dickkopf protein family which modulates Wnt signaling by binding to LRP5/6 co-receptors. Through this interaction DKK4 manages the balance between cell growth and apoptosis important in both early development and adult tissue maintenance. DKK4 presence is often necessary to modulate signals that govern differentiation and proliferation in complex tissue systems.

Pathways

DKK4 predominantly interacts within the Wnt signaling pathway. This pathway is critical for regulating gene expression involved in stem cell maintenance and differentiation. DKK4 acts by interacting with proteins such as LRP6 and Kremen thereby inhibiting Wnt-induced processes. Through modulation of these factors DKK4 ensures the fine-tuning of signaling cascades necessary for maintaining cellular equilibrium under physiological conditions.

Research associates DKK4 with malignant melanoma and colorectal cancer. These associations arise due to DKK4's role in modulating Wnt signaling which can drive oncogenic processes if dysregulated. In melanoma DKK4 can affect tumor cell proliferation by interacting with proteins like beta-catenin. In colorectal cancer abnormal expression of DKK4 might disrupt normal signaling balance further implicating it in tumor growth and progression. These connections highlight DKK4's potential as both a biomarker and a therapeutic target.
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Product protocols

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Target data

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).
See full target information Dickkopf-related protein 4
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