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AB324139

Human DKKL1 ELISA Kit

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Human DKKL1 ELISA Kit is a Sandwich ELISA for the measurement of Human DKKL1 in Human in Cell/Tissue Extracts samples.

View Alternative Names

SGY1, UNQ735/PRO1429, DKKL1, Dickkopf-like protein 1, Cancer/testis antigen 34, Protein soggy-1, CT34, SGY-1

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Sandwich ELISA - Human DKKL1 ELISA Kit (AB324139)
  • sELISA

Supplier Data

Sandwich ELISA - Human DKKL1 ELISA Kit (AB324139)

Example of human DKKL1 standard curve 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human DKKL1 ELISA Kit (AB324139)
  • sELISA

Supplier Data

Sandwich ELISA - Human DKKL1 ELISA Kit (AB324139)

Interpolated concentration of native DKKL1 was measured in duplicate at different sample concentrations. Undiluted samples are 500 ug/mL human testes tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.191 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human DKKL1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DKKL1 protein in Cell Lysate, Tissue extracts. Quantitate Human DKKL1 with 5.191 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

  • Singlewash protocol reduces assay time to 90 minutes or less.
  • High sensitivity, specificity and reproducibility from superior antibodies.
  • Fully validated in biological samples.
  • 96wells plate breakable into 12 x 8 wells strips, available in 10pack (10 x 96well plates).

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.8" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.7" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "92 - 95 %", "average": "= 93" }, { "sample": "Tissue Extracts", "range": "88 - 99 %", "average": "= 94" } ]
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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab324139-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB324139-CMP09" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB324139-CMP08" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB324139-CMP06" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB324139-CMP05" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB324139-CMP10" }, { "size":"20 x 1 Vial", "name":"Human DKKL1 Lyophilized Recombinant Protein", "number":"AB324139-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human DKKL1 Detector Antibody", "number":"AB324139-CMP02" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB324139-CMP04" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB324139-CMP11" }, { "size":"10 x 600 µL", "name":"10X Human DKKL1 Capture Antibody", "number":"AB324139-CMP01" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab324139-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB324139-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB324139-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB324139-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB324139-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB324139-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human DKKL1 Lyophilized Recombinant Protein", "number":"AB324139-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB324139-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human DKKL1 Detector Antibody", "number":"AB324139-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB324139-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB324139-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human DKKL1 Capture Antibody", "number":"AB324139-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Involved in fertilization by facilitating sperm penetration of the zona pellucida. May promote spermatocyte apoptosis, thereby limiting sperm production. In adults, may reduce testosterone synthesis in Leydig cells. Is not essential either for development or fertility.
See full target information DKKL1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com