Human DLL3 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human DLL3 in Cell Lysate samples.
Colorimetric
Cell Lysate
Sandwich (quantitative)
Human
54.688 - 3500 pg/mL
1h 30m
= 42.301 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).
Delta-like protein 3, Drosophila Delta homolog 3, Delta3, DLL3
Human DLL3 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human DLL3 in Cell Lysate samples.
Colorimetric
Cell Lysate
Sandwich (quantitative)
Human
54.688 - 3500 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 42.301 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 3.1 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 7 |
Sample type | Average % | Range |
---|---|---|
Sample type Cell Lysate | Average % = 101 | Range 97 - 105 % |
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DLL3 also known as Delta-like protein 3 is a member of the Delta/Serrate/Jagged family. This protein has a molecular mass of approximately 64 kDa. Scientists find DLL3 expression primarily in the Golgi apparatus of cells. In healthy tissues DLL3 is mostly present in the central nervous system whereas in abnormal conditions it appears in certain types of cancer cells. The detection of DLL3 expression commonly performed using IHC (immunohistochemistry) highlights its potential as a biomarker in pathological studies.
DLL3 functions mainly in the modulation of the Notch signaling pathway. It does not activate this pathway but acts as an inhibitor blocking Notch ligand activation on adjacent cells. DLL3's role involves binding directly to Notch receptors altering cellular differentiation and growth processes. Despite being related to the Delta/Serrate/LAG-2 family DLL3 exhibits unique characteristics in its interactions forming part of a regulatory complex different from its family members.
DLL3 engages importantly in the Notch signaling pathway affecting important cellular communications that regulate development and differentiation. Its interaction with proteins of the Notch family specifically the Notch receptors highlights its inhibitory role. Additionally DLL3 intersects with the Wnt signaling pathway though indirect influencing its output by modulating availability of pathway regulators.
DLL3 has significant implications in cancer notably small cell lung cancer (SCLC) and neuroendocrine tumors. Researchers associate increased DLL3 expression with aggressive tumor behavior and poor prognosis. The protein also shows relevance in congenital disorders such as spondylocostal dysostosis where abnormal DLL3 function leads to vertebral segmentation defects. In these contexts DLL3 often relates to proteins involved in the disruption of normal signaling cascades impacting both tumor progression and developmental anomalies.
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Example of human DLL3 standard curve in 1X Cell Extraction Buffer PTR.
Example of human DLL3 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of human DLL3 in cell extract.
Interpolated concentration of native DLL3 was measured in duplicate at different sample concentrations. Undiluted samples are 20 μg/mL TT cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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