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AB300312

Human DLL3 ELISA Kit

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Human DLL3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human DLL3 with a sensitivity of 42.301 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Delta-like protein 3, Drosophila Delta homolog 3, Delta3, DLL3

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Sandwich ELISA - Human DLL3 ELISA Kit (AB300312)
  • sELISA

Supplier Data

Sandwich ELISA - Human DLL3 ELISA Kit (AB300312)

Example of human DLL3 standard curve in 1X Cell Extraction Buffer PTR.

Example of human DLL3 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human DLL3 ELISA Kit (AB300312)
  • sELISA

Supplier Data

Sandwich ELISA - Human DLL3 ELISA Kit (AB300312)

Interpolated concentrations of human DLL3 in cell extract.

Interpolated concentration of native DLL3 was measured in duplicate at different sample concentrations. Undiluted samples are 20 μg/mL TT cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 42.301 pg/mL

Range

54.688 - 3500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "97 - 105 %", "average": "= 101" } ]
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What's included?

{ "values": { "96Tests": { "sellingSize": "96 Tests", "publicAssetCode":"ab300312-96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB300312-CMP23", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB300312-CMP22", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB300312-CMP18", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB300312-CMP19", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB300312-CMP21", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB300312-CMP20", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human DLL3 Lyophilized Recombinant Protein", "number":"AB300312-CMP15", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB300312-CMP16", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human DLL3 Capture Antibody", "number":"AB300312-CMP13", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB300312-CMP24", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB300312-CMP17", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human DLL3 Detector Antibody", "number":"AB300312-CMP14", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DLL3 also known as Delta-like protein 3 is a member of the Delta/Serrate/Jagged family. This protein has a molecular mass of approximately 64 kDa. Scientists find DLL3 expression primarily in the Golgi apparatus of cells. In healthy tissues DLL3 is mostly present in the central nervous system whereas in abnormal conditions it appears in certain types of cancer cells. The detection of DLL3 expression commonly performed using IHC (immunohistochemistry) highlights its potential as a biomarker in pathological studies.
Biological function summary

DLL3 functions mainly in the modulation of the Notch signaling pathway. It does not activate this pathway but acts as an inhibitor blocking Notch ligand activation on adjacent cells. DLL3's role involves binding directly to Notch receptors altering cellular differentiation and growth processes. Despite being related to the Delta/Serrate/LAG-2 family DLL3 exhibits unique characteristics in its interactions forming part of a regulatory complex different from its family members.

Pathways

DLL3 engages importantly in the Notch signaling pathway affecting important cellular communications that regulate development and differentiation. Its interaction with proteins of the Notch family specifically the Notch receptors highlights its inhibitory role. Additionally DLL3 intersects with the Wnt signaling pathway though indirect influencing its output by modulating availability of pathway regulators.

DLL3 has significant implications in cancer notably small cell lung cancer (SCLC) and neuroendocrine tumors. Researchers associate increased DLL3 expression with aggressive tumor behavior and poor prognosis. The protein also shows relevance in congenital disorders such as spondylocostal dysostosis where abnormal DLL3 function leads to vertebral segmentation defects. In these contexts DLL3 often relates to proteins involved in the disruption of normal signaling cascades impacting both tumor progression and developmental anomalies.
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Product protocols

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Target data

Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).
See full target information DLL3
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websiteProtocolBooklet
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Product promise

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