AB314726
Human DNase I ELISA Kit is a Sandwich ELISA for the measurement of Human DNase I in Human in Biofluids, Cell Culture Media samples.
View Alternative Names
DNL1, DRNI, DNASE1, Deoxyribonuclease-1, Deoxyribonuclease I, DNase I
1 Images
- sELISA
Supplier Data
Sandwich ELISA - Human DNase I ELISA Kit (AB314726)
Example of standard curve using ab314726. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
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Primary Antibodies
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Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
Product details
Human DNase I ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human DNase I in serum (human DNase I concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma (collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended in this assay), and cell culture supernatants. This assay employs an antibody specific for human DNase I coated on a 96-well plate.
Recovery
[ { "sample": "Serum", "range": "71 - 96 %", "average": "= 84.83" }, { "sample": "Plasma", "range": "76 - 139 %", "average": "= 113.2" }, { "sample": "Cell culture media", "range": "111 - 137 %", "average": "= 124.6" } ]
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs (PubMed : 11241278, PubMed : 2251263, PubMed : 2277032). Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (By similarity). Among other functions, seems to be involved in cell death by apoptosis (PubMed : 11241278). Binds specifically to G-actin and blocks actin polymerization (By similarity). Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).
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