AB327311
Human DNase I ELISA Kit, Chemiluminescent
- SimpleStep Ignite™ ELISA
- Recombinant
- What is this?
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Human DNase I ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human DNase I with higher sensitivity and broader measuring range.
- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 1.37 - 3000 pg/ml with a sensitivity of 0.51 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 1.37 - 3000 pg/ml with a sensitivity of 0.51 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
4 Images
- sELISA
Supplier Data
Sandwich ELISA - Human DNase I ELISA Kit, Chemiluminescent (AB327311)
Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean DNase I concentration was determined to be 4675 pg/mL with a range of 1271 – 12993 pg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human DNase I ELISA Kit, Chemiluminescent (AB327311)
Interpolated concentration of native DNase I was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 25% plasma (citrate) 25% plasma (EDTA) 25% plasma (heparin) 25% and urine 1%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Lab
Sandwich ELISA - Human DNase I ELISA Kit, Chemiluminescent (AB327311)
Standard curve comparison between the Chemiluminescent Human DNase I SimpleStep ELISA and colorimetric Human DNase I SimpleStep ELISA (ab315786) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Lab
Sandwich ELISA - Human DNase I ELISA Kit, Chemiluminescent (AB327311)
Dynamic range comparison between the Chemiluminescent Human DNase I SimpleStep ELISA, colorimetric Human DNase I SimpleStep ELISA (ab315786), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
Product details
Human DNase I ELISA Kit, Chemiluminescent (ab327311) is a rapid single-wash sandwich 55-min ELISA to measure Human DNase I in Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Urine, Cell culture media with a sensitivity of 0.51 pg/mL.
How the assay works
Human DNase I ELISA Kit, Chemiluminescent (ab327311) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human DNase I (ab327311) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-DNase I antibody [EPR25941-34] - BSA and Aside free (Capture) ab309565
- Anti-DNase I antibody [EPR25941-19] - BSA and Aside free (Detector) ab309566
How the assay works
Human DNase I ELISA Kit, Chemiluminescent (ab327311) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human DNase I (ab327311) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-DNase I antibody [EPR25941-34] - BSA and Aside free (Capture) ab309565
- Anti-DNase I antibody [EPR25941-19] - BSA and Aside free (Detector) ab309566
Precision
[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" } ]
Recovery
[ { "sample": "Serum", "range": "93 - 99 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "86 - 93 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "92 - 100 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "83 - 109 %", "average": "= 96" }, { "sample": "Urine", "range": "95 - 107 %", "average": "= 101" }, { "sample": "Cell culture media", "range": "78 - 88 %", "average": "= 82" } ]
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs (PubMed : 11241278, PubMed : 2251263, PubMed : 2277032). Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (By similarity). Among other functions, seems to be involved in cell death by apoptosis (PubMed : 11241278). Binds specifically to G-actin and blocks actin polymerization (By similarity). Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).
Alternative Names
DNL1, DRNI, DNASE1, Deoxyribonuclease-1, Deoxyribonuclease I, DNase I
Product promise
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