Human Dnmt3b ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Dnmt3b in Human in Plasma, Serum, Cell culture supernatant samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.64 - 150 ng/mL
- Sensitivity
- = 0.64 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Functions as a transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells (PubMed:27153398).
Alternative names
DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, M.HsaIIIB, DNMT3B
What's included?
Recommended products
Human Dnmt3b ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Dnmt3b in Human in Plasma, Serum, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Serum, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.64 - 150 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.64 ng/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 115.6 | Range 97 - 132 % |
Sample type Plasma | Average % = 104 | Range 77 - 125 % |
Sample type Cell culture supernatant | Average % = 95.38 | Range 84 - 104 % |
Sample type Cell culture media | Average % = 95.38 | Range 84 - 104 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human Dnmt3b ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD42b in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Dnmt3b coated on a 96-well plate.
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1 product image
Sandwich ELISA - Human Dnmt3b ELISA Kit (ab309416)
Example of standard curve using ab309416. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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