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AB277451

Human Dopa Decarboxylase/DCC ELISA Kit

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Human Dopa Decarboxylase/DCC ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Dopa Decarboxylase/DCC in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

AADC, DDC, Aromatic-L-amino-acid decarboxylase, DOPA decarboxylase

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ELISA - Human Dopa Decarboxylase/DCC ELISA Kit (AB277451)
  • ELISA

Supplier Data

ELISA - Human Dopa Decarboxylase/DCC ELISA Kit (AB277451)

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 34 pg/mL

Range

32.77 - 8000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Dopa Decarboxylase/DCC ELISA Kit (ab277451) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Dopa Decarboxylase/DCC in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human Dopa Decarboxylase/DCC coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Dopa Decarboxylase/DCC present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Dopa Decarboxylase/DCC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Dopa Decarboxylase/DCC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "76 - 145 %", "average": "= 110.6" }, { "sample": "Plasma", "range": "68 - 108 %", "average": "= 83.28" }, { "sample": "Cell culture media", "range": "108 - 143 %", "average": "= 128.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277451-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277451-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277451-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277451-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Dopa Decarboxylase DCC coated Microplate", "number":"AB277451-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human Dopa Decarboxylase/DCC Antibody", "number":"AB277451-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"350X HRP-Streptavidin Concentrate", "number":"AB277451-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Dopa Decarboxylase/DCC standard protein (Lyophilized)", "number":"AB277451-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277451-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DOPA Decarboxylase also known as DDC or aromatic L-amino acid decarboxylase is an enzyme critical in neurotransmitter production. It has a molecular mass of approximately 54 kDa. DOPA Decarboxylase converts L-DOPA to dopamine and 5-HTP to serotonin. This enzyme is expressed in tissues like the brain liver and kidney with high activity levels in the central nervous system.
Biological function summary

DOPA Decarboxylase plays an essential role in the synthesis of neurotransmitters such as dopamine and serotonin. It functions as part of the enzymatic conversion process and is not typically associated with larger complexes. The availability and activity of DDC directly influence the levels of these neurotransmitters affecting various physiological responses.

Pathways

DOPA Decarboxylase participates in the catecholamine biosynthesis pathway. This pathway is important for regulating body functions like mood motivation and fine motor control. DDC activity connects it with proteins such as tyrosine hydroxylase which provides the precursor L-DOPA for dopamine synthesis. Additionally it is involved in the serotonin biosynthesis pathway alongside tryptophan hydroxylase.

DOPA Decarboxylase shows significant relevance to conditions like Parkinson's disease and depression. Reduced DDC activity results in decreased dopamine levels which contribute to Parkinson’s symptoms. In depression altered DDC function affects serotonin levels. Its interaction with proteins like monoamine oxidase also plays a role in these disorders due to their involvement in neurotransmitter metabolism.
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Product protocols

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Target data

Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine and L-5-hydroxytryptophan to serotonin.
See full target information DDC
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websiteProtocolBooklet
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