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AB277076

Human Dopamine beta Hydroxylase ELISA Kit

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Human Dopamine beta Hydroxylase ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Dopamine beta Hydroxylase with a sensitivity of 19.05 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Dopamine beta-hydroxylase, Dopamine beta-monooxygenase, DBH

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Sandwich ELISA - Human Dopamine beta Hydroxylase ELISA Kit (AB277076)
  • sELISA

Supplier Data

Sandwich ELISA - Human Dopamine beta Hydroxylase ELISA Kit (AB277076)

Interpolated concentrations of native Dopamine beta Hydroxylase in human serum and plasma samples.

The concentrations of Dopamine beta Hydroxylase were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : serum (1 : 320), plasma – citrate (1 : 320), plasma – heparin (1 : 320), and plasma – EDTA (1 : 320). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 0.96 μg/ml in serum, 1.05 μg/ml in plasma (citrate), 0.96 μg/ml in plasma (heparin), and 1.06 μg/ml in plasma (EDTA).

Sandwich ELISA - Human Dopamine beta Hydroxylase ELISA Kit (AB277076)
  • sELISA

Supplier Data

Sandwich ELISA - Human Dopamine beta Hydroxylase ELISA Kit (AB277076)

Example of human Dopamine beta Hydroxylase standard curve in Sample Diluent NS.

The Dopamine beta Hydroxylase standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 19.05 pg/mL

Range

78.125 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Dopamine beta Hydroxylase ELISA kit (ab277076) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Dopamine beta Hydroxylase protein in human serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Dopamine beta Hydroxylase with 19.05 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Dopamine beta Hydroxylase is an enzyme that catalyzes the conversion of dopamine to norepinephrine. Dopamine beta Hydroxylase is expressed in noradrenergic neurons. It has been implicated in conditions associated with decision making and addictive drugs. SNPs in the Dopamine beta Hydroxylase gene have been associated with cognition in Schizophrenia patients.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": null }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]
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Recovery

[ { "sample": "EDTA Plasma", "range": null, "average": "= 120" }, { "sample": "Heparin Plasma", "range": null, "average": "= 94" }, { "sample": "Citrate plasma", "range": null, "average": "= 109" }, { "sample": "Saliva", "range": null, "average": "= 101" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277076-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB277076-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB277076-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB277076-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB277076-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB277076-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB277076-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB277076-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Dopamine beta Hydroxylase Lyophilized Recombinant Protein", "number":"AB277076-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Dopamine beta Hydroxylase Capture Antibody", "number":"AB277076-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Dopamine beta Hydroxylase Detector Antibody", "number":"AB277076-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Catalyzes the hydroxylation of dopamine to noradrenaline (also known as norepinephrine), and is thus vital for regulation of these neurotransmitters.
See full target information Dopamine beta-hydroxylase
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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