Human Doublecortin ELISA Kit

Product details

Human Doublecortin ELISA Kit (ab218267) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Doublecortin protein in tissue extracts and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Doublecortin with 2.13 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Doublecortin is a microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. It may act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. Doublecortin may in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. Doublecortin may be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration. Doublecortin interacts with tubulin and USP9X. Mutations affecting the Doublecortin gene are causing X-linked 1 Lissencephaly, a disease characterized by mental retardation and seizures that are more severe in male patients. Female patients display a less severe phenotype referred to as 'doublecortex'. Doublecortin is highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, it is highly expressed in the brain frontal lobe, but it has very low expression in other regions of brain, and it is not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.

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