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AB267618

Human DPP2 ELISA Kit (DPP7)

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Human DPP2 ELISA Kit (DPP7) is a Sandwich ELISA for the measurement of Human DPP2 (DPP7) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

DPP2, QPP, DPP7, Dipeptidyl peptidase 2, Dipeptidyl aminopeptidase II, Dipeptidyl peptidase 7, Dipeptidyl peptidase II, Quiescent cell proline dipeptidase, DPP II

1 Images
Sandwich ELISA - Human DPP2 ELISA Kit (DPP7) (AB267618)
  • sELISA

Unknown

Sandwich ELISA - Human DPP2 ELISA Kit (DPP7) (AB267618)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.11 ng/mL

Range

0.102 - 25 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human DPP2 ELISA Kit (DPP7) is designed for the quantitative determination of DPP2 in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human DPP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and DPP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human DPP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DPP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "91 - 112 %", "average": "= 101.1" }, { "sample": "Plasma", "range": "75 - 117 %", "average": "= 90.53" }, { "sample": "Cell culture media", "range": "81 - 108 %", "average": "= 92.39" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267618-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267618-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human DPP2 coated Microplate (12 x 8 wells)", "number":"AB267618-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267618-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin Concentrate", "number":"AB267618-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267618-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human DPP2 Detection Antibody", "number":"AB267618-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human DPP2 Standard (Lyophilized)", "number":"AB267618-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB267618-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB267618-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dipeptidyl peptidase 2 (DPP2) also known as DPP7 is a serine protease enzyme with a molecular mass of approximately 55 kDa. This enzyme hydrolyzes dipeptides from the N-terminus of polypeptides with a preference for sequences with proline or alanine in the penultimate position. DPP2 is located in the cytoplasmic subcellular regions of cells. It is expressed in various tissues including the spleen thymus and other organs involved in hematopoiesis and immune response.
Biological function summary

DPP2 has an important role in cellular processes related to quiescence and apoptosis managing protein turnover and regulation of the cell cycle. It forms part of larger protein complexes that modulate the intracellular protein degradation system and peptide hormone processing. Furthermore DPP2 contributes to immune function through its regulation of lymphocyte quiescence maintaining cells in a resting state until activation is required.

Pathways

DPP2 takes an active part in the metabolism of regulatory peptides and is involved in immune response pathways. It interacts with proteins such as DPP4 which shares some functional similarities participating in immunomodulatory processes. DPP2's actions are integrated into the broader networks of proteolysis and peptide signal modulation influencing cell communication dynamics and immune surveillance activities.

DPP2 has connections to immune-related conditions and certain types of cancer. Links to leukemia have emerged partly through its role as an apoptosis regulator and potential influence on hematopoietic differentiation. The enzyme is also observed in metabolic disorders due to its similarity with DPP4 a known target for addressing conditions like Type 2 diabetes. Its modulation of immune cell function and apoptosis highlights its significance as a therapeutic target in disease settings where these processes are disrupted.
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Product protocols

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Target data

Plays an important role in the degradation of some oligopeptides.
See full target information DPP7
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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