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AB252365

Human DPP4 (CD26) ELISA Kit

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Human DPP4 (CD26) ELISA Kit is a single-wash 90-min Simplestep used to quantify Human DPP4 (CD26) with a sensitivity of 12.82 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD26, ADCP2, DPP4, Dipeptidyl peptidase 4, ADABP, Adenosine deaminase complexing protein 2, Dipeptidyl peptidase IV, T-cell activation antigen CD26, TP103, ADCP-2, DPP IV

5 Images
Sandwich ELISA - Human DPP4 (CD26) ELISA Kit (AB252365)
  • sELISA

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Sandwich ELISA - Human DPP4 (CD26) ELISA Kit (AB252365)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD26 concentration was determined to be 300.8 ng/mL with a range of 200.7 – 421.5 ng/mL.

Sandwich ELISA - Human DPP4 (CD26) ELISA Kit (AB252365)
  • sELISA

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Sandwich ELISA - Human DPP4 (CD26) ELISA Kit (AB252365)

Example of human CD26 standard curve in Sample Diluent NS.

The CD26 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human DPP4 (CD26) ELISA Kit (AB252365)
  • sELISA

Unknown

Sandwich ELISA - Human DPP4 (CD26) ELISA Kit (AB252365)

Interpolated concentrations of native CD26 in human male urine, female saliva, PBMC cell culture supernatant stimulated for 46 hours with 1.5% PHA-M, and HepG2 cell culture supernatant samples.

The concentrations of CD26 were measured in duplicates, interpolated from the CD26 standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 12.5%, saliva 50%, PBMC supernatant 100%, and HepG2 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD26 concentration was determined to be 36.1 ng/mL in urine, 13.5 ng/ml in saliva, 1.0 ng/ml in stimulated PBMC supernatant, and 5.8 ng/ml in HepG2 supernatant.

Sandwich ELISA - Human DPP4 (CD26) ELISA Kit (AB252365)
  • sELISA

Unknown

Sandwich ELISA - Human DPP4 (CD26) ELISA Kit (AB252365)

Human CD26 Standard Curve Comparison.

Standard Curve comparison between human CD26 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human DPP4 (CD26) ELISA Kit (AB252365)
  • sELISA

Unknown

Sandwich ELISA - Human DPP4 (CD26) ELISA Kit (AB252365)

Interpolated concentrations of native CD26 in human serum and plasma samples.

The concentrations of CD26 were measured in duplicates, interpolated from the CD26 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 100, plasma (citrate) 1 : 100, plasma (heparin) 1 : 100, and plasma (EDTA) 1 : 100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD26 concentration was determined to be 570.0 ng/mL in serum, 470.9 ng/mL in plasma (citrate), 514.4 ng/ml in plasma (heparin), and 524.8 ng/ml in plasma (EDTA).

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 12.82 pg/mL

Range

78.13 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human CD26 ELISA Kit (ab252365) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD26 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, saliva, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD26 with 12.82 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CD26, also known as DPP4 (Dipeptidyl Peptidase IV), is a glycoprotein receptor essential for T cell receptor-mediated T cell activation. CD26 is a serine protease that cleaves Xaa-Pro dipeptides from the N terminus of oligo-and polypeptides. Involved in a variety of cellular processes, CD26 is a therapeutic target in clinical trials for type 2 diabetes.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "88 - 106 %", "average": "= 98" }, { "sample": "Serum", "range": "100 - 104 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "95 - 111 %", "average": "= 101" }, { "sample": "Urine", "range": "102 - 109 %", "average": "= 105" }, { "sample": "Saliva", "range": "91 - 105 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "96 - 106 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "103 - 104 %", "average": "= 103" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab252365-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB252365-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB252365-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB252365-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB252365-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB252365-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB252365-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human DPP4 (CD26) Capture Antibody", "number":"AB252365-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human DPP4 (CD26) Lyophilized Recombinant Protein", "number":"AB252365-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB252365-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human DPP4 (CD26) Detector Antibody", "number":"AB252365-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dipeptidyl peptidase 4 also known as DPP4 or CD26 functions as a serine exopeptidase. This enzyme is involved in cleaving dipeptides from the N-terminus of polypeptides. DPP4 with a mass of approximately 110 kDa is expressed in various tissues such as the liver intestines kidney and lymphocytes. It exists in both a membrane-bound form and a soluble form in blood plasma.
Biological function summary

DPP4 plays a significant role in glucose metabolism and immune function. The enzyme interacts with members of the immune system and regulates proteins such as chemokines growth factors and neuropeptides. DPP4 acts independently and is not known to be part of a larger complex. It modulates biological processes by inactivating peptide hormones and signaling molecules which impacts glucose homeostasis and immune responses.

Pathways

DPP4 impacts the incretin signaling pathway and the immune response pathway. Incretin hormones like glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP) are substrates of DPP4 and play important roles in glucose metabolism. DPP4 also interacts with proteins such as adenosine deaminase (ADA) which impacts T-cell function and immune regulation. The regulation of these pathways by DPP4 influences metabolic and immune health.

DPP4 involvement is relevant in type 2 diabetes and immunological disorders. In type 2 diabetes DPP4 inhibitors modulate the incretin hormones improving glycemic control. Proteins like GLP-1 are affected by DPP4 activity connecting it to diabetes management. In autoimmune diseases altered DPP4 expression can impact immune cell functions influencing conditions such as rheumatoid arthritis. Through its effects on ADA and associated pathways DPP4 can influence both metabolic and immune-related disorders.
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Product protocols

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Target data

Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation (PubMed : 10900005, PubMed : 10951221, PubMed : 11772392, PubMed : 17287217). Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC (PubMed : 10900005, PubMed : 10951221, PubMed : 11772392, PubMed : 14691230). Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner (PubMed : 17287217). Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion (PubMed : 11772392). In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM (PubMed : 10593948, PubMed : 16651416). May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation (PubMed : 18708048). When overexpressed, enhanced cell proliferation, a process inhibited by GPC3 (PubMed : 17549790). Also acts as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones such as brain natriuretic peptide 32 (PubMed : 10570924, PubMed : 16254193). Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline (PubMed : 10593948).. (Microbial infection) Acts as a receptor for human coronavirus MERS-CoV-2.
See full target information DPP4
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websiteProtocolBooklet
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Product promise

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