JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB272206

Human DPT ELISA Kit (TRAMP)

Be the first to review this product! Submit a review

|

(0 Publication)

Human DPT ELISA Kit (TRAMP) is a single-wash 90-min Simplestep used to quantify Human DPT (TRAMP) with a sensitivity of 2.94 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Dermatopontin, Tyrosine-rich acidic matrix protein, TRAMP, DPT

3 Images
ELISA - Human DPT ELISA Kit (TRAMP) (AB272206)
  • ELISA

Supplier Data

ELISA - Human DPT ELISA Kit (TRAMP) (AB272206)
Sandwich ELISA - Human DPT ELISA Kit (TRAMP) (AB272206)
  • sELISA

Supplier Data

Sandwich ELISA - Human DPT ELISA Kit (TRAMP) (AB272206)

Example of human DPT standard curve in Sample Diluent NS.

The DPT standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human DPT ELISA Kit (TRAMP) (AB272206)
  • sELISA

Supplier Data

Sandwich ELISA - Human DPT ELISA Kit (TRAMP) (AB272206)

Interpolated concentrations of native DPT in human serum and plasma (citrate) samples.

The concentrations of DPT were measured in duplicates, interpolated from the DPT standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 100 and plasma (citrate) 1 : 100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DPT concentration was determined to be 53.79 ng/mL in serum and 63.04 ng/mL in plasma (citrate).

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Citrate plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.94 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB275907

Human COMP ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human COMP ELISA Kit (AB275907)

  • 0
  • 0
Primary Antibodies

AB255823

Anti-DPT/TRAMP antibody [EPR22845-83]

RabMAb|Recombinant

Western blot - Anti-DPT/TRAMP antibody [EPR22845-83] (AB255823)

  • 0
  • 0
Primary Antibodies

AB263947

Anti-STAT6 (phospho Y641) antibody [EPR22599-78]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (AB263947)

  • 5
  • 1
Multiplex Kits

AB134001

Human Apoptosis Antibody Array - Membrane (43 Targets)

Multiplex Protein Detection - Human Apoptosis Antibody Array - Membrane (43 Targets) (AB134001)

  • 1
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human DPT ELISA Kit (TRAMP) ab272206 is a rapid single-wash 90-min Sandwich ELISA to measure Human DPT (TRAMP)in cell culture media, citrate plasma, serum. This SimpleStep sensitivity is 2.94 pg/mL.

How the assay works

Human DPT (TRAMP)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human DPT ELISA Kit (TRAMP) ab272206 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-DPT/TRAMP antibody [EPR22845-33] - BSA and Azide free (Capture) ab272411
- Anti-DPT/TRAMP antibody [EPR22845-41] - BSA and Azide free (Detector) ab272411

DPT (TRAMP or Dermatopontin) is a matrix protein found in the intracellular junction of the extracellular matrix. DPT is increased in myocardial infarction and decreased in fibrosis and leiomyoma. DPT is a biomarker for giant-cell tumor of the bone and oral squamous cell carcinoma.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": null, "average": "= 107" }, { "sample": "Cell culture media", "range": null, "average": "= 100" }, { "sample": "Citrate plasma", "range": null, "average": "= 102" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab272206-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB272206-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB272206-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB272206-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB272206-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB272206-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB272206-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human DPT (TRAMP) Lyophilized Recombinant Protein", "number":"AB272206-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB272206-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human DPT (TRAMP) Capture Antibody", "number":"AB272206-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human DPT (TRAMP) Detector Antibody", "number":"AB272206-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DPT also known as Dermatopontin is a protein with a molecular mass of approximately 22 kDa. It is expressed in various tissues with high expression levels in the skin heart and lungs. Dermatopontin interacts with extracellular matrix components and influences cell adhesion and matrix organization. The protein plays a role in regulating fibroblast activity and collagen binding making it important in connective tissue structure and function.
Biological function summary

Dermatopontin contributes to maintaining dermal structural integrity and facilitating wound healing. As part of a larger protein interaction network it enhances cellular communication and tissue repair processes. Dermatopontin modulates the bioavailability of TGF-beta which is essential for collagen production and tissue homeostasis. This highlights its presence in skin remodeling and repair mechanisms linking it to new tissue formation.

Pathways

Dermatopontin participates actively in the TGF-beta signaling pathway and the ECM-receptor interaction pathway. These pathways are critical for cellular communication and tissue structuring processes. The protein interacts with Integrins and Fibronectin facilitating cell-to-ECM adhesion and remodeling activities. Consequently Dermatopontin's pathway involvement supports dynamic tissue responses such as healing and functional adaptation to environmental changes.

Dermatopontin shows associations with fibrosis and keloids. These disorders feature aberrant tissue formation and scarring wherein DPT's regulation of fibroblasts and collagen dynamics plays a notable role. Additionally Dermatopontin's interactions with other proteins like Collagen VI and Decorin influence its involvement in these conditions contributing to the understanding of pathological tissue remodeling mechanisms.
style
left-column

Product protocols

style
left-column

Target data

Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation (By similarity).
See full target information Dermatopontin
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com