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AB46013

Human DR5 ELISA Kit (CD262)

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(1 Publication)

Human DR5 ELISA Kit (CD262) is a Sandwich (quantitative) ELISA for the measurement of Human DR5 (CD262) in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

CD262, DR5, KILLER, TRAILR2, TRICK2, ZTNFR9, UNQ160/PRO186, TNFRSF10B, Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, TNF-related apoptosis-inducing ligand receptor 2, TRAIL receptor 2, TRAIL-R2

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Sandwich ELISA - Human DR5 ELISA Kit (CD262) (AB46013)
  • sELISA

Supplier Data

Sandwich ELISA - Human DR5 ELISA Kit (CD262) (AB46013)

Representative Standard Curve using ab46013

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 6 pg/mL

Range

31.25 - 1000 pg/mL

Assay time

2h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's DR5 (CD262) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of DR5 in Human serum, plasma, buffered solutions and cell culture media.

A monoclonal antibody specific for DR5 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known DR5 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for DR5 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of DR5 present in the samples.

This kit will recognize both endogenous and recombinant Human DR5.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 9, "mean": "679 pg/mL", "standardDeviation": "45", "coefficientOfVariability": "6.58" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 9, "mean": "186 pg/mL", "standardDeviation": "14", "coefficientOfVariability": "7.44" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 8, "mean": "483 pg/mL", "standardDeviation": "7", "coefficientOfVariability": "1.39" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 8, "mean": "117 pg/mL", "standardDeviation": "4", "coefficientOfVariability": "3.23" } ]
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What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46013-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46013-CMP06" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46013-CMP08" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46013-CMP01" }, { "size":"2 x 0.4 mL", "name":"Biotinylated anti-DR5 (CD262)", "number":"AB46013-CMP04" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46013-CMP07" }, { "size":"2 x 1 Unit", "name":"DR5 Microplate (12 x 8 well strips)", "number":"AB46013-CMP02" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46013-CMP10" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46013-CMP09" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46013-CMP05" }, { "size":"4 x 1 Vial", "name":"DR5 Standard (Lyophilized)", "number":"AB46013-CMP03" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46013-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46013-CMP06", "productcode":"" }, { "size":"1 x 0.4 mL", "name":"Biotinylated anti-DR5 (CD262)", "number":"AB46013-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"DR5 Microplate (12 x 8 well strips)", "number":"AB46013-CMP02", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46013-CMP07", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46013-CMP10", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46013-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"DR5 Standard (Lyophilized)", "number":"AB46013-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46013-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46013-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46013-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DR5 also known as Death Receptor 5 TRAIL-R2 or TNFRSF10B is a protein critical in apoptosis. It is a transmembrane receptor with a mass of approximately 54 kDa. Researchers find it expressed mostly in tissues such as lung colon and prostate. DR5 functions by binding to its ligand TRAIL triggering the extrinsic apoptotic pathway. Through this mechanism DR5 initiates a cascade of caspase activation that leads to cell death.
Biological function summary

DR5 plays a significant role in the regulation of apoptosis particularly in cancer cells. It is a component of the TRAIL receptor complex which includes several other death receptors like DR4. This complex formation allows DR5 to mediate apoptotic signals more efficiently. By controlling apoptosis DR5 helps maintain tissue homeostasis and prevents abnormal cell proliferation.

Pathways

DR5 is an important part of the TRAIL signaling pathway and the extrinsic apoptosis pathway. It connects with proteins like FADD and Caspase-8 essential in the apoptotic signaling cascade. By interacting with these proteins DR5 drives the progression of the apoptotic signal ensuring the removal of dysfunctional cells. Such pathways are vital for restraining tumor development and progression.

DR5 exhibits significant relevance to cancer and autoimmune diseases. Its ability to induce apoptosis through the TRAIL pathway keeps oncogenic transformations in check. In cancer DR5 often interfaces with other proteins like Bcl-2 which are involved in cell survival. Furthermore defects or aberrant expressions in DR5 have associations with autoimmune disorders where excessive cell death or survival contributes to disease pathology. DR5's role in these conditions makes it a promising target in therapeutic strategies.
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Product protocols

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Target data

Receptor for the cytotoxic ligand TNFSF10/TRAIL (PubMed : 10549288). The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.
See full target information TNFRSF10B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 8:4233-4241 PubMed27830007

2016

Novel markers for circulating tumor stem cells in colorectal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qinghui Meng,Weihua Wu,Tiemin Pei,Long Li,Xiaolong Tang,Haobo Sun
View all publications
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websiteProtocolBooklet
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