Human DSCAML1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human DSCAML1 in Human in Cell culture supernatant, Plasma, Serum samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.82 - 200 ng/mL
- Sensitivity
- = 0.82 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell adhesion molecule that plays a role in neuronal self-avoidance (PubMed:11453658). Promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Promotes both isoneuronal self-avoidance for creating an orderly neurite arborization in retinal rod bipolar cells and heteroneuronal self-avoidance to maintain mosaic spacing between AII amacrine cells (By similarity). Adhesion molecule that promotes lamina-specific synaptic connections in the retina: expressed in specific subsets of interneurons and retinal ganglion cells (RGCs) and promotes synaptic connectivity via homophilic interactions (By similarity).
Alternative names
DSCAM2, KIAA1132, DSCAML1, Cell adhesion molecule DSCAML1, Down syndrome cell adhesion molecule 2, Down syndrome cell adhesion molecule-like protein 1
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Human DSCAML1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human DSCAML1 in Human in Cell culture supernatant, Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.82 - 200 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.82 ng/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 117.7 | Range 94 - 134 % |
Sample type Plasma | Average % = 76.97 | Range 70 - 86 % |
Sample type Cell culture media | Average % = 142.6 | Range 135 - 147 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human DSCAML1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human DSCAML1 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human DSCAML1 coated on a 96-well plate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein DSCAML1 also known as Down syndrome cell adhesion molecule-like 1 plays a mechanical role in cell adhesion and signaling. This protein with a mass of approximately 220 kDa belongs to the immunoglobulin superfamily. DSCAML1 is expressed in various tissues most notably in the nervous system where it helps in the development of neural circuits. The target is known for its complex structure which allows it to mediate homophilic cell-cell interactions essential for neuronal positioning and axonal pathfinding.
- Biological function summary
DSCAML1 is involved in neural patterning and synaptic connectivity. The protein contributes to neural migration processes during development ensuring proper brain morphogenesis. Research suggests that DSCAML1 forms part of a molecular complex that aids in axon guidance important for network formation within the brain. Together with other cell adhesion molecules DSCAML1 influences synapse formation and stability impacting neural networks' functional integrity.
- Pathways
DSCAML1 participates in signaling pathways critical for nervous system development. It significantly influences axon guidance and synaptic signaling pathways. DSCAML1 interacts with other proteins like DSCAM and DCC (Deleted in Colorectal Cancer) which are known to play roles in cellular communication and neuronal growth processes. These interactions contribute to the proteins' collective ability to orchestrate developmental and functional aspects of the central nervous system.
- Associated diseases and disorders
DSCAML1 mutations have associations with neurodevelopmental disorders such as autism spectrum disorders and intellectual disabilities. Alterations in DSCAML1 functions may disrupt neural circuitry leading to these conditions. Proteins like DSCAM whose gene duplication or mutations often occur in Down syndrome share pathways or functions with DSCAML1 and disruptions in these interactions stand linked to altered neural development and related cognitive concerns.
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Sandwich ELISA - Human DSCAML1 ELISA Kit (ab313687)
Example of standard curve using ab313687. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
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Product protocols
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