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AB233611

Human E-Cadherin ELISA Kit

5

(1 Review)

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(17 Publications)

Human E-Cadherin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human E-Cadherin with a sensitivity of 29.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations

View Alternative Names

CD324, CDHE, UVO, CDH1, Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin

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ELISA - Human E-Cadherin ELISA Kit (AB233611)
  • ELISA

Supplier Data

ELISA - Human E-Cadherin ELISA Kit (AB233611)
Sandwich ELISA - Human E-Cadherin ELISA Kit (AB233611)
  • sELISA

Supplier Data

Sandwich ELISA - Human E-Cadherin ELISA Kit (AB233611)

The concentrations of E-Cadherin were measured in duplicates, interpolated from the E-Cadherin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 6.25%, plasma (EDTA) 12%, plasma (citrate) 12%, and plasma (heparin) 12%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 42 ng/mL in serum, 36 ng/mL in plasma (EDTA), and 38 ng/mL plasma (heparin) and 34 ng/mL in plasma (citrate).

Sandwich ELISA - Human E-Cadherin ELISA Kit (AB233611)
  • sELISA

Supplier Data

Sandwich ELISA - Human E-Cadherin ELISA Kit (AB233611)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 52 ng/mL with a range of 10 – 84 ng/mL.

Sandwich ELISA - Human E-Cadherin ELISA Kit (AB233611)
  • sELISA

Lab

Sandwich ELISA - Human E-Cadherin ELISA Kit (AB233611)

Standard curve comparison between Human E-Cadherin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human E-Cadherin ELISA Kit (AB233611)
  • sELISA

Supplier Data

Sandwich ELISA - Human E-Cadherin ELISA Kit (AB233611)

Interpolated concentrations of native E-Cadherin in human saliva.

The concentrations of E-Cadherin were measured in duplicate and interpolated from the E-Cadherin standard curve and corrected for sample dilution. Undiluted samples are as follows : saliva 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 5 ng/mL in saliva.

Sandwich ELISA - Human E-Cadherin ELISA Kit (AB233611)
  • sELISA

Supplier Data

Sandwich ELISA - Human E-Cadherin ELISA Kit (AB233611)

The E-Cadherin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human E-Cadherin ELISA Kit (AB233611)
  • sELISA

Supplier Data

Sandwich ELISA - Human E-Cadherin ELISA Kit (AB233611)

The concentrations of E-Cadherin were measured in duplicate and interpolated from the E-Cadherin standard curve and corrected for sample dilution. Undiluted samples are as follows : MCF-7 cell culture supernatant 100%, saliva 6.25%, and urine 3%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 1.9 ng/mL in MCF-7 supernatant, 5 ng/mL in saliva, and 58 ng/mL in urine.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 29.8 pg/mL

Range

78.1 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human E-Cadherin ELISA Kit ab233611 is a rapid single-wash 90-min Sandwich ELISA to measure Human E-Cadherin in cell culture media, citrate plasma, EDTA plasma, heparin plasma, saliva, serum, urine. This SimpleStep sensitivity is 29.8 pg/mL.

How the assay works

Human E-Cadherin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human E-Cadherin ELISA Kit ab233611 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
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Precision

[ { "reproducibilityType": "Inter", "sample": "MCF-7", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.6" }, { "reproducibilityType": "Intra", "sample": "MCF-7", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.6" } ]
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Recovery

[ { "sample": "Serum", "range": "92 - 104 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "106 - 111 %", "average": "= 108" }, { "sample": "Urine", "range": "104 - 107 %", "average": "= 105" }, { "sample": "Saliva", "range": "90 - 96 %", "average": "= 93" }, { "sample": "Cell culture media", "range": "98 - 103 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "92 - 109 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "93 - 98 %", "average": "= 97" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.
Biological function summary

E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.

Pathways

E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.

E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.
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Product protocols

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Target data

CDH1 encodes a protein that is a calcium-dependent cell adhesion molecule, playing a role in cell-cell adhesion, mobility, and proliferation in epithelial cells. It acts as a potent invasive suppressor and serves as a ligand for integrin alpha-E/beta-7. The protein contributes to the sorting of heterogeneous cell types due to its homophilic interactions. E-Cad/CTF2, a product of CDH1, promotes the non-amyloidogenic degradation of Abeta precursors and strongly inhibits the production of APP C99 and C83. Additionally, in the context of microbial infection, it functions as a receptor for Listeria monocytogenes, with internalin A binding to it to promote bacterial uptake. This supplementary information is collated from multiple sources and compiled automatically.
See full target information CDH1
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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 15:157 PubMed38733530

2024

circPDK1 competitively binds miR-4731-5p to mediate GIGYF1 expression and increase paclitaxel sensitivity in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

YunYin Feng,TaoLong Zhang,Hong Liu

Cell death discovery 10:190 PubMed38653740

2024

OTUB1/NDUFS2 axis promotes pancreatic tumorigenesis through protecting against mitochondrial cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Dong Huang,Li Du,Xiao-Chen Cheng,Yu-Xin Lu,Qiao-Wei Liu,Yi-Wu Wang,Ya-Jin Liao,Dong-Dong Lin,Feng-Jun Xiao

Oncology letters 25:207 PubMed37123020

2023

Increased soluble E‑cadherin of spheroid formation supplemented with fetal bovine serum in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

In-Youb Chang,Sang-Pil Yoon

JHEP reports : innovation in hepatology 5:100722 PubMed37151732

2023

Increased sinusoidal pressure impairs liver endothelial mechanosensing, uncovering novel biomarkers of portal hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Martí Ortega-Ribera,Albert Gibert-Ramos,Laia Abad-Jordà,Marta Magaz,Luis Téllez,Lorena Paule,Elisa Castillo,Raül Pastó,Bruno de Souza Basso,Pol Olivas,Lara Orts,Juan José Lozano,Rosa Villa,Jaime Bosch,Agustín Albillos,Joan Carles García-Pagán,Jordi Gracia-Sancho

Evidence-based complementary and alternative medicine : eCAM 2022:4026688 PubMed36588592

2022

Caudatin Inhibits the Proliferation, Invasion, and Glycolysis of Osteosarcoma Cells via the Wnt/- Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhang,Zhizhong Sheng,Chi Su,Yuli Xia,Xiangfan Chen,Xiaobo Huang,Honglin Li,Changsheng Ma,Ligang Wang

Current research in toxicology 3:100088 PubMed36176311

2022

Lactobacilli metabolites restore E-cadherin and suppress MMP9 in cervical cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Krupali Pawar,Clara Aranha

Translational oncology 24:101491 PubMed35882167

2022

CEACAM5 targeted by miR-498 promotes cell proliferation, migration and epithelial to mesenchymal transition in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zhang,Chao Zhang,Nian Liu

Frontiers in oncology 12:813809 PubMed35280775

2022

COL5A2 Inhibits the TGF-β and Wnt/β-Catenin Signaling Pathways to Inhibit the Invasion and Metastasis of Osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Long Han,Dan Luo,Kakeng Habaxi,Julaiti Tayierjiang,Wei Zhao,Wei Wang,Wumaierjiang Aikebaier,Li Wang

Disease markers 2021:6256369 PubMed34616498

2021

E2F1-Induced lncRNA BAIAP2-AS1 Overexpression Contributes to the Malignant Progression of Hepatocellular Carcinoma via miR-361-3p/SOX4 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Yang,Hong Ge,De-Qing Li,Ai-Xia Xu

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 30:727-736 PubMed34118143

2021

Baicalein modulates the radiosensitivity of cervical cancer cells in vitro via miR-183 and the JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hongwei Lei,Jingbin Shi,Yun Teng,Chenghui Song,Lijuan Zou,Fuxiu Ye,Haichen Zhang
View all publications
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