Human E-Cadherin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human E-Cadherin with a sensitivity of 29.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations
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- Cell death discovery 10:1902024OTUB1/NDUFS2 axis promotes pancreatic tumorigenesis through protecting against mitochondrial cell death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Dong Huang,Li Du,Xiao-Chen Cheng,Yu-Xin Lu,Qiao-Wei Liu,Yi-Wu Wang,Ya-Jin Liao,Dong-Dong Lin,Feng-Jun XiaoPubMed 38653740
- Oncology letters 25:2072023Increased soluble E‑cadherin of spheroid formation supplemented with fetal bovine serum in colorectal cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIn-Youb Chang,Sang-Pil YoonPubMed 37123020
- JHEP reports : innovation in hepatology 5:1007222023Increased sinusoidal pressure impairs liver endothelial mechanosensing, uncovering novel biomarkers of portal hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartí Ortega-Ribera,Albert Gibert-Ramos,Laia Abad-Jordà,Marta Magaz,Luis Téllez,Lorena Paule,Elisa Castillo,Raül Pastó,Bruno de Souza Basso,Pol Olivas,Lara Orts,Juan José Lozano,Rosa Villa,Jaime Bosch,Agustín Albillos,Joan Carles García-Pagán,Jordi Gracia-SanchoPubMed 37151732
- Evidence-based complementary and alternative medicine : eCAM 2022:40266882022Caudatin Inhibits the Proliferation, Invasion, and Glycolysis of Osteosarcoma Cells via the Wnt/- Catenin Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Zhang,Zhizhong Sheng,Chi Su,Yuli Xia,Xiangfan Chen,Xiaobo Huang,Honglin Li,Changsheng Ma,Ligang WangPubMed 36588592
- Current research in toxicology 3:1000882022Lactobacilli metabolites restore E-cadherin and suppress MMP9 in cervical cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKrupali Pawar,Clara AranhaPubMed 36176311
- Translational oncology 24:1014912022CEACAM5 targeted by miR-498 promotes cell proliferation, migration and epithelial to mesenchymal transition in gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Zhang,Chao Zhang,Nian LiuPubMed 35882167
- Frontiers in oncology 12:8138092022COL5A2 Inhibits the TGF-β and Wnt/β-Catenin Signaling Pathways to Inhibit the Invasion and Metastasis of Osteosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan-Long Han,Dan Luo,Kakeng Habaxi,Julaiti Tayierjiang,Wei Zhao,Wei Wang,Wumaierjiang Aikebaier,Li WangPubMed 35280775
- Disease markers 2021:62563692021E2F1-Induced lncRNA BAIAP2-AS1 Overexpression Contributes to the Malignant Progression of Hepatocellular Carcinoma via miR-361-3p/SOX4 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Yang,Hong Ge,De-Qing Li,Ai-Xia XuPubMed 34616498
- Advances in clinical and experimental medicine : official organ Wroclaw Medical University 30:727-7362021Baicalein modulates the radiosensitivity of cervical cancer cells in vitro via miR-183 and the JAK2/STAT3 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongwei Lei,Jingbin Shi,Yun Teng,Chenghui Song,Lijuan Zou,Fuxiu Ye,Haichen ZhangPubMed 34118143
- International journal of molecular sciences 22:2021Association of MALAT1 and PVT1 Variants, Expression Profiles and Target miRNA-101 and miRNA-186 with Colorectal Cancer: Correlation with Epithelial-Mesenchymal Transition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbdullah F Radwan,Olfat G Shaker,Noha A El-Boghdady,Mahmoud A SenousyPubMed 34200314
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 78.1 - 5000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 29.8 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
CDH1 encodes a protein that is a calcium-dependent cell adhesion molecule, playing a role in cell-cell adhesion, mobility, and proliferation in epithelial cells. It acts as a potent invasive suppressor and serves as a ligand for integrin alpha-E/beta-7. The protein contributes to the sorting of heterogeneous cell types due to its homophilic interactions. E-Cad/CTF2, a product of CDH1, promotes the non-amyloidogenic degradation of Abeta precursors and strongly inhibits the production of APP C99 and C83. Additionally, in the context of microbial infection, it functions as a receptor for Listeria monocytogenes, with internalin A binding to it to promote bacterial uptake. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
CD324, CDHE, UVO, CDH1, Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin
What's included?
Recommended products
Human E-Cadherin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human E-Cadherin with a sensitivity of 29.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 78.1 - 5000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 29.8 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample MCF-7 | n 5 | C.V. 7.6 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample MCF-7 | n 3 | C.V. 7.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 97 | Range 92 - 104 % |
Sample type EDTA Plasma | Average % = 108 | Range 106 - 111 % |
Sample type Urine | Average % = 105 | Range 104 - 107 % |
Sample type Saliva | Average % = 93 | Range 90 - 96 % |
Sample type Cell culture media | Average % = 100 | Range 98 - 103 % |
Sample type Heparin Plasma | Average % = 103 | Range 92 - 109 % |
Sample type Citrate plasma | Average % = 97 | Range 93 - 98 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human E-Cadherin ELISA Kit (ab233611) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of E-Cadherin protein in cit plasma, edta plasma, hep plasma, saliva, serum, urine, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human E-Cadherin with 78 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.
- Biological function summary
E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.
- Pathways
E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.
- Associated diseases and disorders
E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.
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6 product images
Sandwich ELISA - Human E-Cadherin ELISA Kit (ab233611)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 52 ng/mL with a range of 10 – 84 ng/mL.
Sandwich ELISA - Human E-Cadherin ELISA Kit (ab233611)
Interpolated concentrations of native E-Cadherin in human saliva.
The concentrations of E-Cadherin were measured in duplicate and interpolated from the E-Cadherin standard curve and corrected for sample dilution. Undiluted samples are as follows: saliva 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 5 ng/mL in saliva.
Sandwich ELISA - Human E-Cadherin ELISA Kit (ab233611)
The E-Cadherin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human E-Cadherin ELISA Kit (ab233611)
Standard curve comparison between Human E-Cadherin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human E-Cadherin ELISA Kit (ab233611)
The concentrations of E-Cadherin were measured in duplicate and interpolated from the E-Cadherin standard curve and corrected for sample dilution. Undiluted samples are as follows: MCF-7 cell culture supernatant 100%, saliva 6.25%, and urine 3%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 1.9 ng/mL in MCF-7 supernatant, 5 ng/mL in saliva, and 58 ng/mL in urine.
Sandwich ELISA - Human E-Cadherin ELISA Kit (ab233611)
The concentrations of E-Cadherin were measured in duplicates, interpolated from the E-Cadherin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 6.25%, plasma (EDTA) 12%, plasma (citrate) 12%, and plasma (heparin) 12%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 42 ng/mL in serum, 36 ng/mL in plasma (EDTA), and 38 ng/mL plasma (heparin) and 34 ng/mL in plasma (citrate).
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