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Human E-Cadherin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human E-Cadherin in Saliva, Urine, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Colorimetric
Saliva, Urine, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Sandwich (quantitative)
Human
78.1 - 5000 pg/mL
1h 30m
= 29.8 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.(Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria.
Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin, CDHE, UVO, CDH1
Human E-Cadherin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human E-Cadherin in Saliva, Urine, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin, CDHE, UVO, CDH1
Colorimetric
Saliva, Urine, Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
Sandwich (quantitative)
Human
78.1 - 5000 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 29.8 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample MCF-7 | n 5 | mean - | SD - | C.V. 7.6 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample MCF-7 | n 3 | mean - | SD - | C.V. 7.6 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 97 | Range 92 - 104 % |
Sample type EDTA Plasma | Average % = 108 | Range 106 - 111 % |
Sample type Urine | Average % = 105 | Range 104 - 107 % |
Sample type Saliva | Average % = 93 | Range 90 - 96 % |
Sample type Cell culture media | Average % = 100 | Range 98 - 103 % |
Sample type Heparin Plasma | Average % = 103 | Range 92 - 109 % |
Sample type Citrate plasma | Average % = 97 | Range 93 - 98 % |
Blue Ice
+4°C
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Human E-Cadherin ELISA Kit (ab233611) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of E-Cadherin protein in cit plasma, edta plasma, hep plasma, saliva, serum, urine, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human E-Cadherin with 78 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.
E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.
E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.
E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.
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Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 52 ng/mL with a range of 10 – 84 ng/mL.
Interpolated concentrations of native E-Cadherin in human saliva.
The concentrations of E-Cadherin were measured in duplicate and interpolated from the E-Cadherin standard curve and corrected for sample dilution. Undiluted samples are as follows: saliva 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 5 ng/mL in saliva.
The concentrations of E-Cadherin were measured in duplicates, interpolated from the E-Cadherin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 6.25%, plasma (EDTA) 12%, plasma (citrate) 12%, and plasma (heparin) 12%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 42 ng/mL in serum, 36 ng/mL in plasma (EDTA), and 38 ng/mL plasma (heparin) and 34 ng/mL in plasma (citrate).
Standard curve comparison between Human E-Cadherin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
The concentrations of E-Cadherin were measured in duplicate and interpolated from the E-Cadherin standard curve and corrected for sample dilution. Undiluted samples are as follows: MCF-7 cell culture supernatant 100%, saliva 6.25%, and urine 3%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean E-Cadherin concentration was determined to be 1.9 ng/mL in MCF-7 supernatant, 5 ng/mL in saliva, and 58 ng/mL in urine.
The E-Cadherin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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