AB246524

Human ECM1 ELISA Kit

Name: Human ECM1 ELISA Kit, colorimetric, 90-min ELISA (ab246524) | Abcam
Brand: Abcam Limited
SKU: AB246524
Price: 735 USD
Availability: InStock
Rating: 4 (1 reviews)

Recombinant
SimpleStep
What is this?

(1 Review)

(2 Publications)

Human ECM1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ECM1 with a sensitivity of 36 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Extracellular matrix protein 1, Secretory component p85, ECM1

4 Images

Sandwich ELISA - Human ECM1 ELISA Kit (AB246524)

sELISA

Supplier Data

Sandwich ELISA - Human ECM1 ELISA Kit (AB246524)

Example of human ECM1 standard curve in Sample Diluent NS.

The ECM1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human ECM1 ELISA Kit (AB246524)

Interpolated concentrations of native ECM1 in human serum, plasma.

The concentrations of ECM1 were measured in duplicates, interpolated from the ECM1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 1000, plasma (citrate) 1 : 2000, plasma (EDTA) 1 : 2000, and plasma (heparin) 1 : 1000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ECM1 concentration was determined to be 4064 ng/mL in serum, 9512 ng/mL in plasma (citrate), 10307 ng/mL in plasma (EDTA), and 2989 ng/ml in plasma heparin.

sELISA

Supplier Data

Sandwich ELISA - Human ECM1 ELISA Kit (AB246524)

Interpolated concentrations of native ECM1 in cell culture supernatant.

The concentrations of ECM1 were measured in duplicate, interpolated from the ECM1 standard curves and corrected for sample dilution. Undiluted samples are as follows : MDA-MB-435S supernatant 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ECM1 concentration was determined to be 141 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human ECM1 ELISA Kit (AB246524)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ECM1 concentration was determined to be 2862 ng/mL with a range of 1028 – 4859 ng/mL.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 36 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human ECM1 ELISA Kit (ab246524) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ECM1 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ECM1 with 36 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 24, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "97 - 104 %", "average": "= 101" }, { "sample": "Serum", "range": "104 - 132 %", "average": "= 114" }, { "sample": "EDTA Plasma", "range": "100 - 112 %", "average": "= 109" }, { "sample": "Heparin Plasma", "range": "100 - 109 %", "average": "= 105" }, { "sample": "Citrate plasma", "range": "104 - 113 %", "average": "= 109" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Extracellular matrix protein 1 often called ECM1 is a matrix protein involved in various mechanical functions in the body. This protein weighs approximately 61 kDa and is widely expressed in human tissues including skin bone lung and brain. ECM1 also goes by the name H3300 vector used in some experimental contexts. Being an extracellular matrix (ECM) component ECM1 plays roles in tissue organization influencing matrix proteins' structural integrity.

Biological function summary

ECM1 contributes to cell-matrix interactions and regulates various cell behaviors such as adhesion proliferation and differentiation. It does this by binding to other ECM proteins and is known not to be part of a specific complex. ECM1 facilitates communication between the cellular environment and matrix thereby supporting the architecture and dynamics of tissues. It modulates angiogenesis linking it with processes like wound healing and vascular development.

Pathways

ECM1 functions within the context of key signaling routes such as the PI3K/AKT pathway known for roles in cell growth and survival. ECM1 interacts with other proteins including matrix metalloproteinases contributing to matrix remodeling activities important for cellular migration and invasion. Cumulatively these interactions highlight ECM1’s place in connective tissue organization and regenerative processes.

ECM1’s dysregulation links it with lipoid proteinosis and skin conditions like Atopic Dermatitis. Imbalances in ECM1 expression or function can lead to abnormal ECM assembly influencing these diseases' development. Moreover abnormalities in ECM1 can alter the behavior of related proteins such as collagen impacting skin integrity and permeability. Understanding ECM1’s role could provide insights into therapeutic strategies for these conditions.

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Product protocols

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Target data

Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity.

See full target information Extracellular matrix protein 1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 14:e70089 PubMed40545963

2025

Extracellular Vesicle Proteome Analysis Improves Diagnosis of Recurrence in Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ju-Yong Hyon,Min Woo Kim,Kyung-A Hyun,Yeji Yang,Seongmin Ha,Jee Ye Kim,Young Kim,Sunyoung Park,Hogyeong Gawk,Heaji Lee,Suji Lee,Sol Moon,Eun Hee Han,Jin Young Kim,Ji Yeong Yang,Hyo-Il Jung,Seung Il Kim,Young-Ho Chung

Biomedicines 9: PubMed34440227

2021

Plasma-Based Bioinks for Extrusion Bioprinting of Advanced Dressings.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Del Amo,Arantza Perez-Valle,Miguel Perez-Garrastachu,Ines Jauregui,Noelia Andollo,Jon Arluzea,Pedro Guerrero,Koro de la Caba,Isabel Andia

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human ECM1 ELISA Kit

Sandwich ELISA - Human ECM1 ELISA Kit (AB246524)

Sandwich ELISA - Human ECM1 ELISA Kit (AB246524)

Sandwich ELISA - Human ECM1 ELISA Kit (AB246524)

Sandwich ELISA - Human ECM1 ELISA Kit (AB246524)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise