Human EG-VEGF ELISA Kit (PK1) is a Sandwich (quantitative) ELISA kit for the measurement of Human EG-VEGF (PK1) in Human in Plasma, Cell culture supernatant samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 15.6 - 1000 pg/mL
- Sensitivity
- < 7 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis.
Alternative names
UNQ600/PRO1186, PROK1, Prokineticin-1, Endocrine-gland-derived vascular endothelial growth factor, Mambakine, EG-VEGF
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Human EG-VEGF ELISA Kit (PK1) is a Sandwich (quantitative) ELISA kit for the measurement of Human EG-VEGF (PK1) in Human in Plasma, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 15.6 - 1000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 7 pg/mL
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's Human EG-VEGF (PK1) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human EG-VEGF (PK1) in cell culture supernatants and plasma (heparin, EDTA).
A EG-VEGF (PK1) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for EG-VEGF (PK1) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human EG-VEGF (PK1) amount of sample captured in plate.
This kit may not be sensitive enough to detect EG-VEGF in serum samples and this sample type is not covered by our AbPromise.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EG-VEGF also known as endocrine gland-derived vascular endothelial growth factor or prokineticin 1 is a potent angiogenic mitogen. It typically has a molecular mass of around 9 kDa. EG-VEGF is expressed mainly in steroidogenic tissues such as the ovaries testes adrenal glands and placenta. It targets endothelial cells selectively within these glands inducing various responses like proliferation migration and fenestration. This selectivity makes it distinct from vascular endothelial growth factor A (VEGF-A) which has a broader expression pattern.
- Biological function summary
EG-VEGF plays a critical role in the formation and remodeling of blood vessels specific to endocrine glands. It is not part of a larger protein complex but operates through interaction with its receptor prokineticin receptor 1 (PROKR1). This unique interaction means that it has a specific ligand-receptor activity contributing to the regulation of vascular development especially in tissues undergoing hormonal changes. This makes EG-VEGF significant in contexts where localized and specific angiogenesis is essential.
- Pathways
EG-VEGF is important in the regulation of angiogenesis and endothelial function. It fits into pathways that influence cell signaling associated with vascular development. One such pathway is the hypoxia-inducible factor (HIF) pathway which enhances the expression of EG-VEGF under low oxygen conditions. Another related protein is vascular endothelial growth factor (VEGF) whereby VEGF and EG-VEGF demonstrate distinctive yet occasionally overlapping functions in angiogenesis optimizing vascular growth within specific environments.
- Associated diseases and disorders
EG-VEGF shows associations with conditions like preeclampsia and polycystic ovary syndrome (PCOS). Preeclampsia involves improper vascularization in the placenta where EG-VEGF's role in angiogenesis becomes critical as it may help or disrupt proper vascular development. Polycystic ovary syndrome a hormonal disorder impacting ovaries also links with EG-VEGF through its effect on ovarian blood vessels. These disorders highlight potential therapeutic targets considering EG-VEGF’s integration with other proteins like PROKR1 which could modulate its angiogenic functions.
Product promise
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1 product image
Sandwich ELISA - Human EG-VEGF ELISA Kit (PK1) (ab119591)
Representative Standard Curve using ab119591.
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