Skip to main content

Human EGF ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human EGF in Human in Plasma, Cell culture supernatant, Serum samples.

Be the first to review this product! Submit a review

Images

Sandwich ELISA - Human EGF ELISA Kit (AB100504), expandable thumbnail
  • Sandwich ELISA - Human EGF ELISA Kit (AB100504), expandable thumbnail
  • Sandwich ELISA - Human EGF ELISA Kit (AB100504), expandable thumbnail

Publications

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.82 - 200 pg/mL

Sensitivity

< 1 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).

Alternative names

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
5X Assay Diluent B
1 x 15 mL
600X HRP-Streptavidin Concentrate
1 x 200 µL
Assay Diluent A
1 x 30 mL
Biotinylated anti-Human EGF
2 x 1 Vial
EGF Microplate (12 strips x 8 wells)
1 x 1 Unit
Recombinant Human EGF Standard (lyophilized)
2 x 1 Vial
Stop Solution
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Human EGF ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human EGF in Human in Plasma, Cell culture supernatant, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.82 - 200 pg/mL

Assay Platform

Microplate

Sensitivity

< 1 pg/mL

General recovery

95%

Recovery

Sample specific recovery

Sample type

Cell culture supernatant

Average %

= 95.52

Range

85 - 106 %

Sample type

Serum

Average %

= 94.52

Range

83 - 104 %

Sample type

Plasma

Average %

= 93.76

Range

84 - 105 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Abcam's EGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human EGF in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human EGF coated on a 96-well plate. Standards and samples are pipetted into the wells and EGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human EGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimisation may be required with urine samples.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Epidermal Growth Factor (EGF) also known as urogastrone is a single polypeptide that facilitates cell growth proliferation and differentiation by binding to the Epidermal Growth Factor Receptor (EGFR). The EGF protein is characterized by a relatively small molecular weight of approximately 6.4 kDa. It is expressed in various tissues including the kidney submandibular glands and Brunner's glands in the mouse and other species. The presence of anti-EGF antibodies can further enhance the understanding of its mechanical actions and functions in different organisms.

Biological function summary

The epidermal growth factor plays a pivotal role in the regulation of cell signaling. It functions outside a complex interacting mainly with EGFR located on the cell surface. Upon ligand binding EGF induces the receptor dimerization leading to autophosphorylation which activates intrinsic intracellular signaling cascades. This activation is critical for cellular responses such as DNA synthesis and the progression of the cell cycle. EGF recombinant proteins such as HEGF are utilized in laboratories to examine its biological activity meticulously.

Pathways

EGF engages in key cellular pathways that mediate various essential functions. It significantly activates the MAPK and PI3K-AKT signaling pathways which govern processes such as cell division and survival. These pathways require the participation of several other proteins including Ras Raf and PDK1 which help modulate their downstream effects. The interplay of EGF with associated proteins highlights its importance in maintaining proper signaling dynamics necessary for healthy cellular function. EGF ELISA kits as well as EGF ELISAs are tools developed for robust analysis of its pathway interactions in research and clinical settings.

Associated diseases and disorders

Altered EGF signaling is associated with increased risk of certain cancers and inflammatory diseases. Overexpression or mutations in the EGF-EGFR axis can lead to the progression of carcinomas such as those found in the lung and breast as well as disorders like psoriasis. These aberrant signaling events are often linked with other proteins such as HER2 and VEGF which can exacerbate disease severity. The study and detection of EGF products therefore offer significant insights into both the progression and potential therapeutic targeting of these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Human EGF ELISA Kit (ab100504), expandable thumbnail

    Sandwich ELISA - Human EGF ELISA Kit (ab100504)

  • Sandwich ELISA - Human EGF ELISA Kit (ab100504), expandable thumbnail

    Sandwich ELISA - Human EGF ELISA Kit (ab100504)

    Standard curve in Diluent A : mean of duplicates (+/-SD)

  • Sandwich ELISA - Human EGF ELISA Kit (ab100504), expandable thumbnail

    Sandwich ELISA - Human EGF ELISA Kit (ab100504)

    Standard curve in Diluent B : mean of duplicates (+/-SD)

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com