Human EGF ELISA Kit ab217772 is a single-wash 90-min SimpleStep ELISA used to quantify Human EGF with a sensitivity of 1.04 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human EGF sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well for higher throughput
- Looking for more flexibility? We also offer antibody pairs ready for conjugation
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- Molecular carcinogenesis 62:1935-19462023Downregulation of cellular retinoic acid binding protein 1 fosters epithelial-mesenchymal transition in thyroid cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Chiung Hsu,Wen-Chien Huang,Chi-Yu Kuo,Ying-Syuan Li,Shih-Ping ChengPubMed 37642311
- International journal of molecular sciences 24:2023Intraocular Pressure-Lowering and Retina-Protective Effects of Exosome-Rich Conditioned Media from Human Amniotic Membrane Stem Cells in a Rat Model of Glaucoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHye-Rim Seong,Chan Ho Noh,Sangryong Park,Sumin Cho,Seok-Jin Hong,Ah-Young Lee,Dongho Geum,Soon-Cheol Hong,Dongsun Park,Tae Myoung Kim,Ehn-Kyoung Choi,Yun-Bae KimPubMed 37175778
- Journal of clinical medicine 11:2021Association between Enzyme-Linked Immunosorbent Assay-Measured Kidney Injury Markers and Urinary Cadmium Levels in Chronic Kidney Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai-Fan Tsai,Pai-Chin Hsu,Chien-Te Lee,Chia-Te Kung,Yi-Chin Chang,Lung-Ming Fu,Yu-Che Ou,Kuo-Chung Lan,Tzung-Hai Yen,Wen-Chin LeePubMed 35011897
- Molecular medicine reports 23:2021Effect of long non‑coding RNA AK021443 on promoting hepatic fibrosis .Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuxin Yang et. al.PubMed 33495828
- Journal of cellular and molecular medicine 24:3931-39472020Inhibition of PLK4 might enhance the anti-tumour effect of bortezomib on glioblastoma via PTEN/PI3K/AKT/mTOR signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Wang,Dengpeng Ren,Yan Sun,Chao Xu,Chunhong Wang,Rui Cheng,Lina Wang,Guijun Jia,Jinrui Ren,Jiuhong Ma,Yue Tu,Hongming JiPubMed 32126150
Key facts
- Detection method
Colorimetric
- Sample types
Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
1.562 - 100 pg/mL
- Assay time
1h 30m
- Sensitivity
= 1.04 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
Alternative names
Pro-epidermal growth factor, EGF, EGF
What's included?
Recommended products
Human EGF ELISA Kit ab217772 is a single-wash 90-min SimpleStep ELISA used to quantify Human EGF with a sensitivity of 1.04 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human EGF sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well for higher throughput
- Looking for more flexibility? We also offer antibody pairs ready for conjugation
Key facts
- Detection method
Colorimetric
- Sample types
Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
1.562 - 100 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 1.04 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Human Serum | n 5 | mean - | SD - | C.V. 8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Human Serum | n 3 | mean - | SD - | C.V. 7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Milk | Average % = 96 | Range 81 - 121 % |
Sample type Serum | Average % = 108 | Range 93 - 122 % |
Sample type EDTA Plasma | Average % = 116 | Range 104 - 127 % |
Sample type Urine | Average % = 103 | Range 101 - 105 % |
Sample type Saliva | Average % = 92 | Range 90 - 96 % |
Sample type Heparin Plasma | Average % = 109 | Range 104 - 114 % |
Sample type Citrate plasma | Average % = 112 | Range 100 - 126 % |
Sample type Cell culture media | Average % = 91 | Range 88 - 95 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human EGF ELISA Kit (ab217772) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of EGF protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, milk, saliva, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human EGF with 1.04 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
EGF precursor is a large 1185 amino acid group 1 member of the EGF family growth factors. All EGF family growth factors are synthesized as transmembrane proteins that undergo proteolytic processing to generate soluble forms. Human EGF is a 53 amino acid long fragment of the extracellular domain of EGF precursor. As other group 1 members, EGF binds to, and activates the EGF receptor. This promotes EGFR dimerization. EGF has variety of diverse physiological effects. EGF activity is complicated by the fact that other EGF family members utilize EGF receptor. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. EGF is proposed to affect growth and/or differentiation of many other fetal and adult tissues.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Epidermal Growth Factor (EGF) also known as urogastrone is a single polypeptide that facilitates cell growth proliferation and differentiation by binding to the Epidermal Growth Factor Receptor (EGFR). The EGF protein is characterized by a relatively small molecular weight of approximately 6.4 kDa. It is expressed in various tissues including the kidney submandibular glands and Brunner's glands in the mouse and other species. The presence of anti-EGF antibodies can further enhance the understanding of its mechanical actions and functions in different organisms.
- Biological function summary
The epidermal growth factor plays a pivotal role in the regulation of cell signaling. It functions outside a complex interacting mainly with EGFR located on the cell surface. Upon ligand binding EGF induces the receptor dimerization leading to autophosphorylation which activates intrinsic intracellular signaling cascades. This activation is critical for cellular responses such as DNA synthesis and the progression of the cell cycle. EGF recombinant proteins such as HEGF are utilized in laboratories to examine its biological activity meticulously.
- Pathways
EGF engages in key cellular pathways that mediate various essential functions. It significantly activates the MAPK and PI3K-AKT signaling pathways which govern processes such as cell division and survival. These pathways require the participation of several other proteins including Ras Raf and PDK1 which help modulate their downstream effects. The interplay of EGF with associated proteins highlights its importance in maintaining proper signaling dynamics necessary for healthy cellular function. EGF ELISA kits as well as EGF ELISAs are tools developed for robust analysis of its pathway interactions in research and clinical settings.
- Associated diseases and disorders
Altered EGF signaling is associated with increased risk of certain cancers and inflammatory diseases. Overexpression or mutations in the EGF-EGFR axis can lead to the progression of carcinomas such as those found in the lung and breast as well as disorders like psoriasis. These aberrant signaling events are often linked with other proteins such as HER2 and VEGF which can exacerbate disease severity. The study and detection of EGF products therefore offer significant insights into both the progression and potential therapeutic targeting of these conditions.
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9 product images
Sandwich ELISA - Human EGF ELISA Kit (ab217772)
Example of human EGF standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human EGF ELISA Kit (ab217772)
Example of human EGF standard curve in Sample Diluent 50BP.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human EGF ELISA Kit (ab217772)
Interpolated concentrations of native EGF in saliva, urine, and serum samples.
The concentrations of EGF were measured in duplicates, interpolated from the EGF standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 2.5%, urine 0.4% and serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EGF concentration was determined to be 5377 pg/mL in neat saliva, 25147 pg/mL in neat urine, and 101 pg/mL in neat serum.
Sandwich ELISA - Human EGF ELISA Kit (ab217772)
Interpolated concentrations of native EGF in whole and de-fatted human breast milk samples.
The concentrations of EGF were measured in duplicates, interpolated from the EGF standard curves and corrected for sample dilution. Undiluted samples are as follows: whole milk 100%, de-fatted milk 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EGF concentration was determined to be 18,596 pg/mL in neat whole milk and 19,757 pg/mL in neat de-fatted milk.
Sandwich ELISA - Human EGF ELISA Kit (ab217772)
Interpolated concentrations of spiked EGF.
Interpolated concentrations of a 200 pg/mL recombinant EGF spike in human platelet poor plasma citrate, platelet poor plasma heparin, platelet poor plasma EDTA, serum, and a 100 pg/mL recombinant EGF spike in RPMI +10% FBS media samples. The concentrations of EGF were measured in duplicates, interpolated from the EGF standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma citrate 50%, plasma heparin 50%, plasma EDTA 50%, serum 50%, and RPMI + 10% FBS 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human EGF ELISA Kit (ab217772)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EGF concentration was determined to be 200 pg/mL with a range of 5.3 – 1436 pg/mL.
Sandwich ELISA - Human EGF ELISA Kit (ab217772)
Interpolated concentration of native EGF was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:250 human urine. The interpolated dilution factor corrected values (to neat) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human EGF ELISA Kit (ab217772)
Example of human EGF standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human EGF ELISA Kit (ab217772)
Example of human EGF standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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