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AB217772

Human EGF ELISA Kit

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(1 Review)

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(7 Publications)

Human EGF ELISA Kit is a single-wash 90-min Simplestep used to quantify Human EGF with a sensitivity of 1.04 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Pro-epidermal growth factor, EGF

10 Images
ELISA - Human EGF ELISA Kit (AB217772)
  • ELISA

Supplier Data

ELISA - Human EGF ELISA Kit (AB217772)
Sandwich ELISA - Human EGF ELISA Kit (AB217772)
  • sELISA

Lab

Sandwich ELISA - Human EGF ELISA Kit (AB217772)

Interpolated concentration of native EGF was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 250 human urine. The interpolated dilution factor corrected values (to neat) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human EGF ELISA Kit (AB217772)
  • sELISA

Supplier Data

Sandwich ELISA - Human EGF ELISA Kit (AB217772)

Interpolated concentrations of native EGF in whole and de-fatted human breast milk samples.

The concentrations of EGF were measured in duplicates, interpolated from the EGF standard curves and corrected for sample dilution. Undiluted samples are as follows : whole milk 100%, de-fatted milk 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EGF concentration was determined to be 18,596 pg/mL in neat whole milk and 19,757 pg/mL in neat de-fatted milk.

Sandwich ELISA - Human EGF ELISA Kit (AB217772)
  • sELISA

Supplier Data

Sandwich ELISA - Human EGF ELISA Kit (AB217772)

Interpolated concentrations of spiked EGF.

Interpolated concentrations of a 200 pg/mL recombinant EGF spike in human platelet poor plasma citrate, platelet poor plasma heparin, platelet poor plasma EDTA, serum, and a 100 pg/mL recombinant EGF spike in RPMI +10% FBS media samples. The concentrations of EGF were measured in duplicates, interpolated from the EGF standard curves and corrected for sample dilution. Undiluted samples are as follows : plasma citrate 50%, plasma heparin 50%, plasma EDTA 50%, serum 50%, and RPMI + 10% FBS 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human EGF ELISA Kit (AB217772)
  • sELISA

Supplier Data

Sandwich ELISA - Human EGF ELISA Kit (AB217772)

Example of human EGF standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human EGF ELISA Kit (AB217772)
  • sELISA

Lab

Sandwich ELISA - Human EGF ELISA Kit (AB217772)

Example of human EGF standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human EGF ELISA Kit (AB217772)
  • sELISA

Lab

Sandwich ELISA - Human EGF ELISA Kit (AB217772)

Example of human EGF standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human EGF ELISA Kit (AB217772)
  • sELISA

Supplier Data

Sandwich ELISA - Human EGF ELISA Kit (AB217772)

Interpolated concentrations of native EGF in saliva, urine, and serum samples.

The concentrations of EGF were measured in duplicates, interpolated from the EGF standard curves and corrected for sample dilution. Undiluted samples are as follows : saliva 2.5%, urine 0.4% and serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EGF concentration was determined to be 5377 pg/mL in neat saliva, 25147 pg/mL in neat urine, and 101 pg/mL in neat serum.

Sandwich ELISA - Human EGF ELISA Kit (AB217772)
  • sELISA

Supplier Data

Sandwich ELISA - Human EGF ELISA Kit (AB217772)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EGF concentration was determined to be 200 pg/mL with a range of 5.3 – 1436 pg/mL.

Sandwich ELISA - Human EGF ELISA Kit (AB217772)
  • sELISA

Supplier Data

Sandwich ELISA - Human EGF ELISA Kit (AB217772)

Example of human EGF standard curve in Sample Diluent 50BP.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.04 pg/mL

Range

1.562 - 100 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human EGF ELISA Kit ab217772 is a rapid single-wash 90-min Sandwich ELISA to measure Human EGF in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, milk, saliva, serum, urine. This SimpleStep sensitivity is 1.04 pg/mL.

How the assay works

Human EGF SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human EGF ELISA Kit ab217772 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Human EGF ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-EGF antibody [EPR19899-62] - BSA and Azide free (Capture) ab242990
- Anti-EGF antibody [EPR19899-46] - BSA and Azide free (Detector) ab242990

EGF precursor is a large 1185 amino acid group 1 member of the EGF family growth factors. All EGF family growth factors are synthesized as transmembrane proteins that undergo proteolytic processing to generate soluble forms. Human EGF is a 53 amino acid long fragment of the extracellular domain of EGF precursor. As other group 1 members, EGF binds to, and activates the EGF receptor. This promotes EGFR dimerization. EGF has variety of diverse physiological effects. EGF activity is complicated by the fact that other EGF family members utilize EGF receptor. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. EGF is proposed to affect growth and/or differentiation of many other fetal and adult tissues.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Human Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" }, { "reproducibilityType": "Intra", "sample": "Human Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8" } ]
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Recovery

[ { "sample": "Milk", "range": "81 - 121 %", "average": "= 96" }, { "sample": "Serum", "range": "93 - 122 %", "average": "= 108" }, { "sample": "EDTA Plasma", "range": "104 - 127 %", "average": "= 116" }, { "sample": "Urine", "range": "101 - 105 %", "average": "= 103" }, { "sample": "Saliva", "range": "90 - 96 %", "average": "= 92" }, { "sample": "Heparin Plasma", "range": "104 - 114 %", "average": "= 109" }, { "sample": "Citrate plasma", "range": "100 - 126 %", "average": "= 112" }, { "sample": "Cell culture media", "range": "88 - 95 %", "average": "= 91" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Epidermal Growth Factor (EGF) also known as urogastrone is a single polypeptide that facilitates cell growth proliferation and differentiation by binding to the Epidermal Growth Factor Receptor (EGFR). The EGF protein is characterized by a relatively small molecular weight of approximately 6.4 kDa. It is expressed in various tissues including the kidney submandibular glands and Brunner's glands in the mouse and other species. The presence of anti-EGF antibodies can further enhance the understanding of its mechanical actions and functions in different organisms.
Biological function summary

The epidermal growth factor plays a pivotal role in the regulation of cell signaling. It functions outside a complex interacting mainly with EGFR located on the cell surface. Upon ligand binding EGF induces the receptor dimerization leading to autophosphorylation which activates intrinsic intracellular signaling cascades. This activation is critical for cellular responses such as DNA synthesis and the progression of the cell cycle. EGF recombinant proteins such as HEGF are utilized in laboratories to examine its biological activity meticulously.

Pathways

EGF engages in key cellular pathways that mediate various essential functions. It significantly activates the MAPK and PI3K-AKT signaling pathways which govern processes such as cell division and survival. These pathways require the participation of several other proteins including Ras Raf and PDK1 which help modulate their downstream effects. The interplay of EGF with associated proteins highlights its importance in maintaining proper signaling dynamics necessary for healthy cellular function. EGF ELISA kits as well as EGF ELISAs are tools developed for robust analysis of its pathway interactions in research and clinical settings.

Altered EGF signaling is associated with increased risk of certain cancers and inflammatory diseases. Overexpression or mutations in the EGF-EGFR axis can lead to the progression of carcinomas such as those found in the lung and breast as well as disorders like psoriasis. These aberrant signaling events are often linked with other proteins such as HER2 and VEGF which can exacerbate disease severity. The study and detection of EGF products therefore offer significant insights into both the progression and potential therapeutic targeting of these conditions.
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Product protocols

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Target data

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed : 10964941).
See full target information EGF
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 44:68 PubMed39994761

2025

Reciprocal regulation of MMP-28 and EGFR is required for sustaining proliferative signaling in PDAC.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengtao Hong,Xing Huang,Linghao Xia,Tingbo Liang,Xueli Bai

Nature communications 15:5010 PubMed38866762

2024

Transient growth factor expression via mRNA in lipid nanoparticles promotes hepatocyte cell therapy in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Anna R Smith,Fatima Rizvi,Elissa Everton,Anisah Adeagbo,Susan Wu,Ying Tam,Hiromi Muramatsu,Norbert Pardi,Drew Weissman,Valerie Gouon-Evans

Molecular carcinogenesis 62:1935-1946 PubMed37642311

2023

Downregulation of cellular retinoic acid binding protein 1 fosters epithelial-mesenchymal transition in thyroid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Chiung Hsu,Wen-Chien Huang,Chi-Yu Kuo,Ying-Syuan Li,Shih-Ping Cheng

International journal of molecular sciences 24: PubMed37175778

2023

Intraocular Pressure-Lowering and Retina-Protective Effects of Exosome-Rich Conditioned Media from Human Amniotic Membrane Stem Cells in a Rat Model of Glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hye-Rim Seong,Chan Ho Noh,Sangryong Park,Sumin Cho,Seok-Jin Hong,Ah-Young Lee,Dongho Geum,Soon-Cheol Hong,Dongsun Park,Tae Myoung Kim,Ehn-Kyoung Choi,Yun-Bae Kim

Journal of clinical medicine 11: PubMed35011897

2021

Association between Enzyme-Linked Immunosorbent Assay-Measured Kidney Injury Markers and Urinary Cadmium Levels in Chronic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Kai-Fan Tsai,Pai-Chin Hsu,Chien-Te Lee,Chia-Te Kung,Yi-Chin Chang,Lung-Ming Fu,Yu-Che Ou,Kuo-Chung Lan,Tzung-Hai Yen,Wen-Chin Lee

Molecular medicine reports 23: PubMed33495828

2021

Effect of long non‑coding RNA AK021443 on promoting hepatic fibrosis .

Applications

Unspecified application

Species

Unspecified reactive species

Yuxin Yang,Zongsu Min

Journal of cellular and molecular medicine 24:3931-3947 PubMed32126150

2020

Inhibition of PLK4 might enhance the anti-tumour effect of bortezomib on glioblastoma via PTEN/PI3K/AKT/mTOR signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Dengpeng Ren,Yan Sun,Chao Xu,Chunhong Wang,Rui Cheng,Lina Wang,Guijun Jia,Jinrui Ren,Jiuhong Ma,Yue Tu,Hongming Ji
View all publications
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