Name: Human EGF ELISA Kit
SKU: ab217772
Rating: 5 (1 reviews)

Human EGF ELISA Kit is a single-wash 90-min Simplestep used to quantify Human EGF with a sensitivity of 1.04 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

Images

ELISA - Human EGF ELISA Kit (AB217772), expandable thumbnail

Publications

Key facts

Detection method: Colorimetric
Sample types: Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 1.562 - 100 pg/mL
Assay time: 1h 30m
Sensitivity: = 1.04 pg/mL

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Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

See full target information EGF

Function

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).

Alternative names

Pro-epidermal growth factor, EGF

What's included?

10 x 96 Tests

Components

10X Human EGF Capture Antibody

10 x 600 µL

10X Wash Buffer PT (ab206977)

1 x 200 mL

Antibody Diluent 4BI

10 x 6 mL

Human EGF Detector Antibody (Lyophilized)

10 x 1 Vial

Human EGF Lyophilized Recombinant Protein

10 x 2 Vial

Plate Seals

10 x 1 Unit

Sample Diluent 50BP

10 x 20 mL

Sample Diluent NS (ab193972)

2 x 250 mL

SimpleStep Pre-Coated 96-Well Microplate (ab206978)

10 x 1 Unit

Stop Solution

1 x 120 mL

TMB Development Solution

1 x 120 mL

Key facts

Detection method: Colorimetric
Sample types: Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 1.562 - 100 pg/mL
Assay time: 1h 30m
Assay Platform: Pre-coated microplate (12 x 8 well strips)
Sensitivity: = 1.04 pg/mL

Precision

Intra assay

Sample	n	C.V.
Sample Human Serum	n 5	C.V. 8

Inter assay

Sample	n	C.V.
Sample Human Serum	n 3	C.V. 7

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Milk	Average % = 96	Range 81 - 121 %
Sample type Serum	Average % = 108	Range 93 - 122 %
Sample type EDTA Plasma	Average % = 116	Range 104 - 127 %
Sample type Urine	Average % = 103	Range 101 - 105 %
Sample type Saliva	Average % = 92	Range 90 - 96 %
Sample type Heparin Plasma	Average % = 109	Range 104 - 114 %
Sample type Citrate plasma	Average % = 112	Range 100 - 126 %
Sample type Cell culture media	Average % = 91	Range 88 - 95 %

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

Human EGF ELISA Kit ab217772 is a rapid single-wash 90-min Sandwich ELISA to measure Human EGF in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, milk, saliva, serum, urine. This SimpleStep sensitivity is 1.04 pg/mL.

How the assay works

Human EGF SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human EGF ELISA Kit ab217772 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Human EGF ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-EGF antibody [EPR19899-62] - BSA and Azide free (Capture) Anti-EGF antibody [EPR19899-46] - BSA and Azide free (Detector) ab242990
- Anti-EGF antibody [EPR19899-46] - BSA and Azide free (Detector) Anti-EGF antibody [EPR19899-46] - BSA and Azide free (Detector) ab242990

EGF precursor is a large 1185 amino acid group 1 member of the EGF family growth factors. All EGF family growth factors are synthesized as transmembrane proteins that undergo proteolytic processing to generate soluble forms. Human EGF is a 53 amino acid long fragment of the extracellular domain of EGF precursor. As other group 1 members, EGF binds to, and activates the EGF receptor. This promotes EGFR dimerization. EGF has variety of diverse physiological effects. EGF activity is complicated by the fact that other EGF family members utilize EGF receptor. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. EGF is proposed to affect growth and/or differentiation of many other fetal and adult tissues.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Epidermal Growth Factor (EGF) also known as urogastrone is a single polypeptide that facilitates cell growth proliferation and differentiation by binding to the Epidermal Growth Factor Receptor (EGFR). The EGF protein is characterized by a relatively small molecular weight of approximately 6.4 kDa. It is expressed in various tissues including the kidney submandibular glands and Brunner's glands in the mouse and other species. The presence of anti-EGF antibodies can further enhance the understanding of its mechanical actions and functions in different organisms.

Biological function summary

The epidermal growth factor plays a pivotal role in the regulation of cell signaling. It functions outside a complex interacting mainly with EGFR located on the cell surface. Upon ligand binding EGF induces the receptor dimerization leading to autophosphorylation which activates intrinsic intracellular signaling cascades. This activation is critical for cellular responses such as DNA synthesis and the progression of the cell cycle. EGF recombinant proteins such as HEGF are utilized in laboratories to examine its biological activity meticulously.

Pathways

EGF engages in key cellular pathways that mediate various essential functions. It significantly activates the MAPK and PI3K-AKT signaling pathways which govern processes such as cell division and survival. These pathways require the participation of several other proteins including Ras Raf and PDK1 which help modulate their downstream effects. The interplay of EGF with associated proteins highlights its importance in maintaining proper signaling dynamics necessary for healthy cellular function. EGF ELISA kits as well as EGF ELISAs are tools developed for robust analysis of its pathway interactions in research and clinical settings.

Associated diseases and disorders

Altered EGF signaling is associated with increased risk of certain cancers and inflammatory diseases. Overexpression or mutations in the EGF-EGFR axis can lead to the progression of carcinomas such as those found in the lung and breast as well as disorders like psoriasis. These aberrant signaling events are often linked with other proteins such as HER2 and VEGF which can exacerbate disease severity. The study and detection of EGF products therefore offer significant insights into both the progression and potential therapeutic targeting of these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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10 product images

ELISA - Human EGF ELISA Kit (ab217772)
Sandwich ELISA - Human EGF ELISA Kit (ab217772)
Example of human EGF standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human EGF ELISA Kit (ab217772)
Example of human EGF standard curve in Sample Diluent 50BP.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human EGF ELISA Kit (ab217772)
Interpolated concentrations of native EGF in saliva, urine, and serum samples.
The concentrations of EGF were measured in duplicates, interpolated from the EGF standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 2.5%, urine 0.4% and serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EGF concentration was determined to be 5377 pg/mL in neat saliva, 25147 pg/mL in neat urine, and 101 pg/mL in neat serum.
Sandwich ELISA - Human EGF ELISA Kit (ab217772)
Interpolated concentrations of native EGF in whole and de-fatted human breast milk samples.
The concentrations of EGF were measured in duplicates, interpolated from the EGF standard curves and corrected for sample dilution. Undiluted samples are as follows: whole milk 100%, de-fatted milk 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EGF concentration was determined to be 18,596 pg/mL in neat whole milk and 19,757 pg/mL in neat de-fatted milk.
Sandwich ELISA - Human EGF ELISA Kit (ab217772)
Interpolated concentrations of spiked EGF.
Interpolated concentrations of a 200 pg/mL recombinant EGF spike in human platelet poor plasma citrate, platelet poor plasma heparin, platelet poor plasma EDTA, serum, and a 100 pg/mL recombinant EGF spike in RPMI +10% FBS media samples. The concentrations of EGF were measured in duplicates, interpolated from the EGF standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma citrate 50%, plasma heparin 50%, plasma EDTA 50%, serum 50%, and RPMI + 10% FBS 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human EGF ELISA Kit (ab217772)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EGF concentration was determined to be 200 pg/mL with a range of 5.3 – 1436 pg/mL.
Sandwich ELISA - Human EGF ELISA Kit (ab217772)
Interpolated concentration of native EGF was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:250 human urine. The interpolated dilution factor corrected values (to neat) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human EGF ELISA Kit (ab217772)
Example of human EGF standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human EGF ELISA Kit (ab217772)
Example of human EGF standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.