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AB275896

Human EGFL7 ELISA Kit

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Human EGFL7 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human EGFL7 with a sensitivity of 0.11 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

MEGF7, UNQ187/PRO1449, EGFL7, Epidermal growth factor-like protein 7, EGF-like protein 7, Multiple epidermal growth factor-like domains protein 7, NOTCH4-like protein, Vascular endothelial statin, Zneu1, Multiple EGF-like domains protein 7, VE-statin

2 Images
Sandwich ELISA - Human EGFL7 ELISA Kit (AB275896)
  • sELISA

Supplier Data

Sandwich ELISA - Human EGFL7 ELISA Kit (AB275896)

Example of human EGFL7 standard curve in Sample Diluent NS.

The EGFL7 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human EGFL7 ELISA Kit (AB275896)
  • sELISA

Supplier Data

Sandwich ELISA - Human EGFL7 ELISA Kit (AB275896)

Interpolated concentrations of native and spiked EGFL7 in Huvec extract based on a 300 μg/mL extract load.

Huvec extract was spiked with recombinant EGFL7 protein alongside due to the low native expression of the target protein. The concentrations of EGFL7 were measured in duplicate and interpolated from the EGFL7 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.11 ng/mL

Range

0.47 - 30 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human EGFL7 ELISA kit (ab275896) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human EGFL7 protein in human serum, plasma (EDTA), cell culture supernatant, and cell and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human EGFL7 with 0.11 ng/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Epidermal growth factor-like protein 7 (EGFL7), also known as Vascular endothelial statin, NOTCH4-like protein, and Zneu1, is a protein encoded by the gene EGFL7. EGFL7 is expressed as a 273-amino acid protein containing a 22- amino acid N-terminal signal peptide, a 78- amino acid EMI domain, and two EGF-like domains. EGFL7 helps regulate vascular tubulogenesis, inhibits platelet-derived growth factor BB-induced smooth muscle migration, and promotes endothelial cell adhesion to the extracellular matrix.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.3" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.5" } ]
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Recovery

[ { "sample": "Serum", "range": "70 - 76 %", "average": "= 73" }, { "sample": "EDTA Plasma", "range": "78 - 79 %", "average": "= 79" }, { "sample": "Cell culture media", "range": "94 - 98 %", "average": "= 95" }, { "sample": "Cell Lysate", "range": "80 - 82 %", "average": "= 81" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EGFL7 also known as Epidermal Growth Factor-like Domain 7 is a secreted protein with a molecular mass of approximately 31 kDa. Scientists observe its expression mainly in vascular endothelial cells indicating its role in vasculature. It characterizes itself by its distinctive EGF-like repeat domains which enable interaction with other proteins in the cellular environment.
Biological function summary

EGFL7 plays an essential role in blood vessel development and maintenance. It is involved in the modulation of endothelial cell behavior primarily through cell proliferation migration and organization. EGFL7 influences angiogenesis by affecting the extracellular matrix environment but it does not form part of a larger protein complex. It serves as a regulator by interacting directly with other components of the angiogenic process.

Pathways

Research has established EGFL7 as a significant participant in the angiogenesis and Notch signaling pathways. It interacts closely with proteins such as COX-2 within the angiogenic pathway and through its efficacy impacts the downstream signaling events in vessels' formation and repair. The proper functioning of these pathways highlights the importance of EGFL7 in vascular biology.

Researchers associate EGFL7 with certain cancers and cardiovascular diseases. Elevated levels of EGFL7 in tumors implicate it in tumor angiogenesis and metastasis suggesting its potential as a biomarker or therapeutic target. In cardiovascular contexts its interaction with VEGF suggests a role in pathologies involving vascular dysfunctions including atherosclerosis.
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Product protocols

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Target data

Regulates vascular tubulogenesis in vivo. Inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cell adhesion to the extracellular matrix and angiogenesis.
See full target information EGFL7
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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