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Human EGFR ELISA Kit (ERBB) is a sandwich ELISA designed to quantify Human EGFR (ERBB) with a sensitivity of 4 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 2.74 - 2000 pg/mL

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Images

Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (AB100505), expandable thumbnail
  • Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (AB100505), expandable thumbnail
  • Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (AB100505), expandable thumbnail
  • Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (AB100505), expandable thumbnail

Publications

Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell culture supernatant, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
2.74 - 2000 pg/mL
Sensitivity
< 4 pg/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:10805725, PubMed:27153536, PubMed:2790960, PubMed:35538033). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:12297049, PubMed:15611079, PubMed:17909029, PubMed:20837704, PubMed:27153536, PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536). May also activate the NF-kappa-B signaling cascade (PubMed:11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed:11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed:11483589). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed:20462955). Plays a role in enhancing learning and memory performance (By similarity). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (By similarity). Isoform 2 may act as an antagonist of EGF action. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.

Alternative names

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
300X HRP-Streptavidin Concentrate
1 x 200 µL
5X Assay Diluent B
1 x 15 mL
Assay Diluent A
1 x 30 mL
Biotinylated anti-Human EGFR
2 x 1 Vial
EGFR Microplate (12 x 8 well strips)
1 x 1 Unit
Recombinant Human EGFR Standard (lyophilized)
2 x 1 Vial
Stop Solution
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Human EGFR ELISA Kit (ERBB) is a sandwich ELISA designed to quantify Human EGFR (ERBB) with a sensitivity of 4 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 2.74 - 2000 pg/mL

Key facts

Detection method
Colorimetric
Sample types
Plasma, Cell culture supernatant, Serum
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
2.74 - 2000 pg/mL
Assay Platform
Microplate
Sensitivity
< 4 pg/mL
General recovery
> 85%

Recovery

Sample specific recovery

Sample type
Cell culture supernatant
Average %
= 85.65
Range
72 - 96 %
Sample type
Serum
Average %
= 105.4
Range
94 - 116 %
Sample type
Plasma
Average %
= 107.2
Range
96 - 119 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Abcam's EGFR (ERBB) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human EGFR in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human EGFR coated on a 96-well plate. Standards and samples are pipetted into the wells and EGFR present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman EGFR antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EGFR bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get higher sensitivity in only 90 minutes with Human soluble EGFR ELISA Kit (ab193764) from our SimpleStep ELISA® range.

Optimisation may be required with urine samples.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.

Biological function summary

The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.

Pathways

EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.

Associated diseases and disorders

EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (ab100505), expandable thumbnail

    Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (ab100505)

    EGFR measured in cell lysates showing quantity (ng) per 1 million cells

  • Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (ab100505), expandable thumbnail

    Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (ab100505)

    Representative Standard Curve using ab100505.

  • Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (ab100505), expandable thumbnail

    Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (ab100505)

    EGFR measured in various fluids showing quantity (ng) per mL of tested sample

  • Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (ab100505), expandable thumbnail

    Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (ab100505)

    Representative Standard Curve using ab100505.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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