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AB100505

Human EGFR ELISA Kit (ERBB)

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(4 Publications)

Human EGFR ELISA Kit (ERBB) is a sandwich ELISA designed to quantify Human EGFR (ERBB) with a sensitivity of 4 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 2.74 - 2000 pg/mL

View Alternative Names

ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

4 Images
Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (AB100505)
  • sELISA

Supplier Data

Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (AB100505)

Representative Standard Curve using ab100505.

Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (AB100505)
  • sELISA

Supplier Data

Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (AB100505)

Representative Standard Curve using ab100505.

Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (AB100505)
  • sELISA

Lab

Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (AB100505)

EGFR measured in various fluids showing quantity (ng) per mL of tested sample

Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (AB100505)
  • sELISA

Lab

Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (AB100505)

EGFR measured in cell lysates showing quantity (ng) per 1 million cells

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 4 pg/mL

Range

2.74 - 2000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human EGFR ELISA Kit (ERBB) ab100505 is a sandwich ELISA to measure Human EGFR (ERBB) in serum, plasma, cell culture supernatant with a sensitivity of 4 pg/ml.

How the assay works

This assay employs an antibody specific for Human EGFR coated on a 96-well plate. Standards and samples are pipetted into the wells and EGFR present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman EGFR antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EGFR bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get higher sensitivity in only 90 minutes with Human soluble EGFR ELISA Kit (ab193764) from our SimpleStep ELISA® range.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human EGFR ELISA Kit (ERBB) ab100505 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.

Get results in 90 minutes with our SimpleStep ELISA® kit

Human soluble EGFR ELISA Kit (ab193764)

Optimisation may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "72 - 96 %", "average": "= 85.65" }, { "sample": "Serum", "range": "94 - 116 %", "average": "= 105.4" }, { "sample": "Plasma", "range": "96 - 119 %", "average": "= 107.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100505-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100505-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100505-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100505-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human EGFR Standard (lyophilized)", "number":"AB100505-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100505-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"EGFR Microplate (12 x 8 well strips)", "number":"AB100505-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human EGFR", "number":"AB100505-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100505-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB100505-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
Biological function summary

The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.

Pathways

EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.

EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
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Product protocols

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Target data

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed : 10805725, PubMed : 27153536, PubMed : 2790960, PubMed : 35538033). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed : 12297049, PubMed : 15611079, PubMed : 17909029, PubMed : 20837704, PubMed : 27153536, PubMed : 2790960, PubMed : 7679104, PubMed : 8144591, PubMed : 9419975). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed : 27153536). May also activate the NF-kappa-B signaling cascade (PubMed : 11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed : 11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed : 11483589). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed : 20462955). Plays a role in enhancing learning and memory performance (By similarity). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (By similarity).. Isoform 2 may act as an antagonist of EGF action.. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.
See full target information EGFR
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:23743 PubMed40610477

2025

Discovery and molecular characterization of a potent thiazolyl-pyrazole hybrid targeting EGFR for breast cancer therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Samar E Mahmoud,Ahmed A Fadda,Ehab Abdel-Latif,Mohamed R Elmorsy

ACS nano 17:8108-8122 PubMed37129374

2023

Simultaneous Detection of Tumor Derived Exosomal Protein-MicroRNA Pairs with an Exo-PROS Biosensor for Cancer Diagnosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chang-Chieh Hsu,Yunchen Yang,Eric Kannisto,Xie Zeng,Guan Yu,Santosh K Patnaik,Grace K Dy,Mary E Reid,Qiaoqiang Gan,Yun Wu

Aging 12:5516-5538 PubMed32224504

2020

Microarray analysis of verbenalin-treated human amniotic epithelial cells reveals therapeutic potential for Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Farhana Ferdousi,Shinji Kondo,Kazunori Sasaki,Yoshiaki Uchida,Nobuhiro Ohkohchi,Yun-Wen Zheng,Hiroko Isoda

ACS sensors 3:1471-1479 PubMed30019892

2018

Sensitive Detection of Exosomal Proteins via a Compact Surface Plasmon Resonance Biosensor for Cancer Diagnosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Liu,Xie Zeng,Zijian An,Yunchen Yang,Maxwell Eisenbaum,Xiaodong Gu,Josep M Jornet,Grace K Dy,Mary E Reid,Qiaoqiang Gan,Yun Wu
View all publications
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Product promise

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