Human EGFR ELISA Kit (ERBB) is a sandwich ELISA designed to quantify Human EGFR (ERBB) with a sensitivity of 4 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 2.74 - 2000 pg/mL
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- ACS nano 17:8108-81222023Simultaneous Detection of Tumor Derived Exosomal Protein-MicroRNA Pairs with an Exo-PROS Biosensor for Cancer Diagnosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChang-Chieh Hsu,Yunchen Yang,Eric Kannisto,Xie Zeng,Guan Yu,Santosh K Patnaik,Grace K Dy,Mary E Reid,Qiaoqiang Gan,Yun WuPubMed 37129374
- Aging 12:5516-55382020Microarray analysis of verbenalin-treated human amniotic epithelial cells reveals therapeutic potential for Alzheimer's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFarhana Ferdousi et. al.PubMed 32224504
- ACS sensors 3:1471-14792018Sensitive Detection of Exosomal Proteins via a Compact Surface Plasmon Resonance Biosensor for Cancer Diagnosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChang Liu,Xie Zeng,Zijian An,Yunchen Yang,Maxwell Eisenbaum,Xiaodong Gu,Josep M Jornet,Grace K Dy,Mary E Reid,Qiaoqiang Gan,Yun WuPubMed 30019892
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2.74 - 2000 pg/mL
- Sensitivity
- < 4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:10805725, PubMed:27153536, PubMed:2790960, PubMed:35538033). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:12297049, PubMed:15611079, PubMed:17909029, PubMed:20837704, PubMed:27153536, PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536). May also activate the NF-kappa-B signaling cascade (PubMed:11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed:11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed:11483589). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed:20462955). Plays a role in enhancing learning and memory performance (By similarity). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (By similarity). Isoform 2 may act as an antagonist of EGF action. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.
Alternative names
ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
What's included?
Recommended products
Human EGFR ELISA Kit (ERBB) is a sandwich ELISA designed to quantify Human EGFR (ERBB) with a sensitivity of 4 pg/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 2.74 - 2000 pg/mL
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 2.74 - 2000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 4 pg/mL
- General recovery
- > 85%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 85.65 | Range 72 - 96 % |
Sample type Serum | Average % = 105.4 | Range 94 - 116 % |
Sample type Plasma | Average % = 107.2 | Range 96 - 119 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's EGFR (ERBB) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human EGFR in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human EGFR coated on a 96-well plate. Standards and samples are pipetted into the wells and EGFR present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman EGFR antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EGFR bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human soluble EGFR ELISA Kit (ab193764) from our SimpleStep ELISA® range.
Optimisation may be required with urine samples.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.
- Biological function summary
The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.
- Pathways
EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.
- Associated diseases and disorders
EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.
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4 product images
Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (ab100505)
EGFR measured in cell lysates showing quantity (ng) per 1 million cells
Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (ab100505)
Representative Standard Curve using ab100505.
Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (ab100505)
EGFR measured in various fluids showing quantity (ng) per mL of tested sample
Sandwich ELISA - Human EGFR ELISA Kit (ERBB) (ab100505)
Representative Standard Curve using ab100505.
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