Human EGFR (pY1086) + total EGFR ELISA Kit
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(1 Publication)
- sELISA
Supplier Data
Sandwich ELISA - Human EGFR (pY1086) + total EGFR ELISA Kit (AB126441)
The A431 cells were treated with 100 ng/ml recombinant human EGF for 20 minutes to induce phosphorylation of EGFR. Serial dilutions of lysates were analyzed in this ELISA.
- sELISA
Supplier Data
Sandwich ELISA - Human EGFR (pY1086) + total EGFR ELISA Kit (AB126441)
A431 cells were treated or untreated with 100 ng/ml recombinant human EGF for 10 min. Cell lysates were analyzed using this phosphoELISA.
- sELISA
Supplier Data
Sandwich ELISA - Human EGFR (pY1086) + total EGFR ELISA Kit (AB126441)
A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.
- WB
Supplier Data
Western blot - Human EGFR (pY1086) + total EGFR ELISA Kit (AB126441)
A431 cells were treated or untreated with 100 ng/ml recombinant human EGF for 10 min. Cell lysates were analyzed by Western Blot
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- WB
Supplier Data
Western blot - Human EGFR (pY1086) + total EGFR ELISA Kit (AB126441)
The A431 cells were treated with 100 ng/ml recombinant human EGF for 20 minutes to induce phosphorylation of EGFR. Serial dilutions of lysates were analyzed by Western blot. Immunoblots were incubated with anti-phospho-EGFR (Y1086).
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Reactivity data
Product details
ab126441 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated EGFR protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-EGFR (Y1086) and total EGFR (help normalize the results of phospho-EGFR from different cell lysates being compared). An anti-EGFR antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and EGFR present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-phosphorylated EGFR (Y1086) or anti-total EGFR antibody is used to detect phosphorylated or non-phosphorylated EGFR. After washing away unbound antibody, HRP-conjugated anti-Rabbit IgG or HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EGFR (Y1086) or total EGFR bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
REACH authorisation
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Properties and storage information
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.
Pathways
EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.
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Publications (1)
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Oncology letters 16:6522-6530 PubMed30405791
2018
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Product promise
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