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AB240679

Human Elafin ELISA Kit

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Human Elafin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Elafin with a sensitivity of 1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

WAP3, WFDC14, PI3, Elafin, Elastase-specific inhibitor, Peptidase inhibitor 3, Protease inhibitor WAP3, Skin-derived antileukoproteinase, WAP four-disulfide core domain protein 14, ESI, PI-3, SKALP

3 Images
Sandwich ELISA - Human Elafin ELISA Kit (AB240679)
  • sELISA

Unknown

Sandwich ELISA - Human Elafin ELISA Kit (AB240679)

Interpolated concentrations of native Elafin in human serum and plasma (EDTA) samples. The concentrations of Elafin were measured in duplicates, interpolated from the Elafin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 160 and plasma (EDTA) 1 : 40. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Elafin concentration was determined to be 2.880 ng/mL in neat serum and 3.362 ng/mL in neat plasma (EDTA).

Sandwich ELISA - Human Elafin ELISA Kit (AB240679)
  • sELISA

Unknown

Sandwich ELISA - Human Elafin ELISA Kit (AB240679)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Elafin concentration was determined to be 2.951 ng/mL with a range of 1.341 - 4.198 ng/mL.

Sandwich ELISA - Human Elafin ELISA Kit (AB240679)
  • sELISA

Unknown

Sandwich ELISA - Human Elafin ELISA Kit (AB240679)

Example of human Elafin standard curve in Sample Diluent 5BS. The Elafin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1 pg/mL

Range

1.9 - 120 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Elafin ELISA Kit (ab240679) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Elafin protein in edta plasma and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Elafin with 1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.6" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.2" } ]
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Recovery

[ { "sample": "Serum", "range": "96 - 117 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "84 - 114 %", "average": "= 97" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab240679-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB240679-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB240679-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB240679-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BS", "number":"AB240679-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Elafin Capture Antibody", "number":"AB240679-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB240679-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Elafin Lyophilized Recombinant Protein", "number":"AB240679-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB240679-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB240679-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"10X Human Elafin Detector Antibody", "number":"AB240679-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB240679-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Elafin also known as ESI or ESI protein is a small serine protease inhibitor with a molecular mass of around 6 kDa. This protein produced by epithelial cells resides predominantly in the skin lungs and other mucosal surfaces. The Elafin protein plays an important mechanical role in inhibiting neutrophil elastase and proteinase 3. It contributes to the regulation of protease activity within tissues controlling inflammation and repair processes.
Biological function summary

Elafin interacts intimately with protease inhibitors to guard tissues against excessive proteolytic damage. It does not typically form large complexes but functions as part of the innate immune defense. This protein helps to neutralize the action of neutrophil elastase which can help maintain the integrity of connective tissue extracellular matrix. Despite its standalone function Elafin's activity serves in tandem with other inhibitors in the protease-antiprotease balance.

Pathways

Elafin integrates prominently into the proteolytic and inflammatory pathways. It functions particularly in the inflammation and immune response pathways coordinating with proteins like secretory leukocyte protease inhibitor (SLPI) and alpha-1 antitrypsin. Elafin inhibits enzymes that degrade connective tissue components. Therefore it plays an important role in modulating inflammatory responses and tissue remodeling post-injury.

Connections between Elafin and conditions such as chronic obstructive pulmonary disease (COPD) and psoriasis appear significant. These diseases relate to abnormal protease activity and chronic inflammation where Elafin's inhibitory function can be affected or diminished. Studies have shown that altered Elafin levels link with these diseases alongside proteins like neutrophil elastase and matrix metalloproteinases which contribute to tissue damage. Researchers continue to explore Elafin's therapeutic potential in managing such inflammatory conditions.
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Product protocols

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Target data

Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. Has been shown to inhibit the alpha-4-beta-2/CHRNA2-CHRNB2 nicotinic acetylcholine receptor and to produce a weak inhibition on Kv11.1/KCNH2/ERG1 and on the transient receptor potential cation channel subfamily V member 1 (TRPV1) (PubMed : 29483648).
See full target information PI3
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websiteProtocolBooklet
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Product promise

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