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AB100658

Human Elafin ELISA Kit (Skalp)

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(3 Publications)

Human Elafin ELISA Kit (Skalp) is a sandwich ELISA designed to quantify Human Elafin (Skalp) with a sensitivity of 5 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 6.86 - 5000 pg/mL

View Alternative Names

WAP3, WFDC14, PI3, Elafin, Elastase-specific inhibitor, Peptidase inhibitor 3, Protease inhibitor WAP3, Skin-derived antileukoproteinase, WAP four-disulfide core domain protein 14, ESI, PI-3, SKALP

1 Images
Sandwich ELISA - Human Elafin ELISA Kit (Skalp) (AB100658)
  • sELISA

Supplier Data

Sandwich ELISA - Human Elafin ELISA Kit (Skalp) (AB100658)

Representative standard curve using ab100658

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5 pg/mL

Range

6.86 - 5000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Elafin ELISA Kit (Skalp) ab100658 is a sandwich ELISA to measure Human Elafin (Skalp) in serum, plasma, cell culture supernatant with a sensitivity of 5 pg/ml.

How the assay works

This assay employs an antibody specific for Human Elafin coated on a 96-well plate. Standards and samples are pipetted into the wells and Elafin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Elafin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Elafin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Elafin ELISA Kit (Skalp) ab100658 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "102 - 121 %", "average": "= 111.8" }, { "sample": "Serum", "range": "105 - 125 %", "average": "= 115.7" }, { "sample": "Plasma", "range": "103 - 122 %", "average": "= 112.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100658-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100658-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100658-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100658-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Elafin Microplate (12 x 8 wells)", "number":"AB100658-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human Elafin Standard (lyophilized)", "number":"AB100658-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human Elafin", "number":"AB100658-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB100658-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100658-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Elafin also known as ESI or ESI protein is a small serine protease inhibitor with a molecular mass of around 6 kDa. This protein produced by epithelial cells resides predominantly in the skin lungs and other mucosal surfaces. The Elafin protein plays an important mechanical role in inhibiting neutrophil elastase and proteinase 3. It contributes to the regulation of protease activity within tissues controlling inflammation and repair processes.
Biological function summary

Elafin interacts intimately with protease inhibitors to guard tissues against excessive proteolytic damage. It does not typically form large complexes but functions as part of the innate immune defense. This protein helps to neutralize the action of neutrophil elastase which can help maintain the integrity of connective tissue extracellular matrix. Despite its standalone function Elafin's activity serves in tandem with other inhibitors in the protease-antiprotease balance.

Pathways

Elafin integrates prominently into the proteolytic and inflammatory pathways. It functions particularly in the inflammation and immune response pathways coordinating with proteins like secretory leukocyte protease inhibitor (SLPI) and alpha-1 antitrypsin. Elafin inhibits enzymes that degrade connective tissue components. Therefore it plays an important role in modulating inflammatory responses and tissue remodeling post-injury.

Connections between Elafin and conditions such as chronic obstructive pulmonary disease (COPD) and psoriasis appear significant. These diseases relate to abnormal protease activity and chronic inflammation where Elafin's inhibitory function can be affected or diminished. Studies have shown that altered Elafin levels link with these diseases alongside proteins like neutrophil elastase and matrix metalloproteinases which contribute to tissue damage. Researchers continue to explore Elafin's therapeutic potential in managing such inflammatory conditions.
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Product protocols

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Target data

Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. Has been shown to inhibit the alpha-4-beta-2/CHRNA2-CHRNB2 nicotinic acetylcholine receptor and to produce a weak inhibition on Kv11.1/KCNH2/ERG1 and on the transient receptor potential cation channel subfamily V member 1 (TRPV1) (PubMed : 29483648).
See full target information PI3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35328414

2022

Compartmentalized Innate Immune Response of Human Fetal Membranes against Choriodecidual Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Olmos-Ortiz,Mayra Hernández-Pérez,Pilar Flores-Espinosa,Gabriela Sedano,Addy Cecilia Helguera-Repetto,Óscar Villavicencio-Carrisoza,María Yolotzin Valdespino-Vazquez,Arturo Flores-Pliego,Claudine Irles,Bruno Rivas-Santiago,Elsa Romelia Moreno-Verduzco,Lorenza Díaz,Verónica Zaga-Clavellina

Scientific reports 7:17022 PubMed29208979

2017

Induction of antimicrobial peptides secretion by IL-1β enhances human amniotic membrane for regenerative medicine.

Applications

ELISA

Species

Unspecified reactive species

Fatemeh A Tehrani,Khashayar Modaresifar,Sara Azizian,Hassan Niknejad

Cryobiology 67:293-8 PubMed23988559

2013

The effects of preservation procedures on antibacterial property of amniotic membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Fatemeh A Tehrani,Abolhassan Ahmadiani,Hassan Niknejad
View all publications
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websiteProtocolBooklet
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Product promise

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