JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB239433

Human Elastin ELISA Kit

Be the first to review this product! Submit a review

|

(5 Publications)

Human Elastin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Elastin with a sensitivity of 44 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Elastin, Tropoelastin, ELN

5 Images
ELISA - Human Elastin ELISA Kit (AB239433)
  • ELISA

Supplier Data

ELISA - Human Elastin ELISA Kit (AB239433)
Sandwich ELISA - Human Elastin ELISA Kit (AB239433)
  • sELISA

Unknown

Sandwich ELISA - Human Elastin ELISA Kit (AB239433)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Elastin concentration was determined to be 2.94 ng/mL with a range of 1.64 – 5.41 ng/mL.

Sandwich ELISA - Human Elastin ELISA Kit (AB239433)
  • sELISA

Lab

Sandwich ELISA - Human Elastin ELISA Kit (AB239433)

Example of human Elastin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Elastin ELISA Kit (AB239433)
  • sELISA

Unknown

Sandwich ELISA - Human Elastin ELISA Kit (AB239433)

Example of human Elastin standard curve in Sample Diluent 50BS.

The Elastin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Elastin ELISA Kit (AB239433)
  • sELISA

Supplier Data

Sandwich ELISA - Human Elastin ELISA Kit (AB239433)

Interpolated concentrations of native Elastin in human serum and plasma samples.

The concentrations of Elastin were measured in duplicates, interpolated from the Elastin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Elastin concentration was determined to be 1.78 ng/mL in neat serum, 2.01 ng/mL in neat plasma (citrate) and 2.26 ng/mL in neat plasma (EDTA).

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 44 pg/mL

Range

0.19 - 12 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB210966

Human Pro-Collagen I alpha 1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)

  • 5
  • 1
Assay Kits

AB100647

Human TGF beta 1 ELISA Kit

Sandwich ELISA - Human TGF beta 1 ELISA Kit (AB100647)

  • 5
  • 2
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB187394

Human TIMP1 ELISA Kit

Recombinant|SimpleStep

ELISA - Human TIMP1 ELISA Kit (AB187394)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Elastin ELISA Kit ab239433 is a rapid single-wash 90-min ELISA kit to measure Human Elastin in serum and plasma. This SimpleStep ELISA® sensitivity is 44 pg/mL.

How the assay works

Human Elastin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit is designed for the quantification of human Elastin. The standard protein in this kit is an N-terminal fragment representing approximately 40% of the full-length mass of mature human Elastin. Native signal was detected in serum, plasma (citrate), and plasma (EDTA) sample types. Spiked protein experiments were used to validate serum, plasma (citrate), and plasma (EDTA) sample types. Cell culture supernatant, saliva, urine, milk, CSF, cell extract, and tissue extract samples have not been tested with this kit. This kit is incompatible with plasma (heparin) samples.

Human Elastin ELISA Kit ab239433 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human Elastin ELISA Kit ab239433

Human Elastin ELISA Kit ab239433 has been used to study biomarkers of psoriasis and aging (1)and skin rejuvenation using engineered extracellular vesicles(2).

References:

(1)Lenka Borska et al. 2022, PMID: 35625870,
(2)Yuan Wan et al. 2019, PMID: 31909348

Save your precious samples

Our 384-well Human Elastin ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Human Elastin Antibody Pair - BSA and Azide free ab256604
- Anti-Elastin antibody [EPR20414-6] - BSA and Azide free (Capture) ab259455
- Anti-Elastin antibody [EPR20414-34] - BSA and Azide free (Detector) ab259456

Related and recommended products

Human Elastin ELISA Kit ab239433 is commonly used to study tissue structure and function. Elastin is a component of the extracellular matrix (ECM).

Other ELISA kits to measure the ECM and its role in tissue function include:

- Human Collagen Type I ELISA kit ab285250
- Human Pro-Collagen I alpha 1 ELISA kit ab210966
- Total Glycosaminoglycans Assay Kit ab289842

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Protein", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" }, { "reproducibilityType": "Intra", "sample": "Protein", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "102 - 107 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "93 - 99 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "90 - 91 %", "average": "= 91" } ]
style
left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab239433-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB239433-CMP07" }, { "size":"10 x 600 µL", "name":"10X Human Elastin Capture Antibody", "number":"AB239433-CMP04" }, { "size":"10 x 20 mL", "name":"Sample Diluent 50BS", "number":"AB239433-CMP03" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB239433-CMP12" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB239433-CMP11" }, { "size":"20 x 1 Vial", "name":"Human Elastin Lyophilized Recombinant Protein", "number":"AB239433-CMP06" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB239433-CMP08" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB239433-CMP10" }, { "size":"10 x 600 µL", "name":"10X Human Elastin Detector Antibody", "number":"AB239433-CMP05" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB239433-CMP09" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB239433-CMP13" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab239433-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB239433-CMP08" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB239433-CMP13" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB239433-CMP12" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BS", "number":"AB239433-CMP03" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB239433-CMP11" }, { "size":"1 x 600 µL", "name":"10X Human Elastin Capture Antibody", "number":"AB239433-CMP04" }, { "size":"2 x 1 Vial", "name":"Human Elastin Lyophilized Recombinant Protein", "number":"AB239433-CMP06" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB239433-CMP10" }, { "size":"1 x 600 µL", "name":"10X Human Elastin Detector Antibody", "number":"AB239433-CMP05" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB239433-CMP09" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB239433-CMP14" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab239433-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB239433-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB239433-CMP12", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB239433-CMP13", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Elastin Capture Antibody", "number":"AB239433-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB239433-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Elastin Lyophilized Recombinant Protein", "number":"AB239433-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BS", "number":"AB239433-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB239433-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Elastin Detector Antibody", "number":"AB239433-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB239433-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB239433-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Elastin also known as elastin protein is an essential component of the extracellular matrix known for imparting elasticity and resilience to tissues. With a molecular mass of approximately 60-70 kDa some scientists may also refer to it as elastin et. Elastin is expressed in elastic tissues such as the skin lungs and blood vessels. These tissues require the elastic properties provided by elastin. Additionally elastin forms the core of elastic fibers surrounded by a sheath of microfibrils.
Biological function summary

Elastin provides mechanical support and elasticity to tissues. It does so by being part of elastic fibers playing an important role in many connective tissue structures. The unique ability to stretch and return to its original shape allows tissues to resume their shape after stretching or contracting. Elastin is not part of larger protein complexes but interacts with other matrix proteins such as fibrillin to form a fibrous network required for the structural integrity of elastic tissues.

Pathways

Elastin plays an integral role in the extracellular matrix remodeling pathway which is important for maintaining tissue structure and function. It also participates in the TGF-beta signaling pathway where elastin interacts indirectly with proteins such as fibrillin-1 and fibulin. These interactions influence cellular processes like proliferation and differentiation highlighting elastin's involvement in both maintaining tissue elasticity and cell signaling.

Elastin is linked to pathologies like Williams-Beuren syndrome and cutis laxa. Williams-Beuren syndrome associated with elastin deletions leads to cardiovascular issues while cutis laxa results in reduced elasticity of the skin due to elastin mutations. In these conditions elastin interacts with proteins such as fibrillin and fibulin contributing to the observed clinical manifestations. Understanding these interactions helps in diagnosing and developing therapeutic approaches for such disorders.
style
left-column

Product protocols

style
left-column

Target data

Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle (By similarity).
See full target information ELN
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutics 17: PubMed39861784

2025

Biotechnological Phytocomplex of (L.) DC. Enhances Collagen Biosynthesis In Vitro and Improves Skin Elasticity In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanna Rigillo,Giovanna Pressi,Oriana Bertaiola,Chiara Guarnerio,Matilde Merlin,Roberto Zambonin,Stefano Pandolfo,Angela Golosio,Francesca Masin,Fabio Tascedda,Marco Biagi,Giulia Baini

Dermatology and therapy 14:2929-2950 PubMed39297898

2024

A New TGF-β Mimetic, XEP™-716 Miniprotein™, Exhibiting Regenerative Properties Objectivized by Instrumental Evaluation.

Applications

Unspecified application

Species

Unspecified reactive species

Hanane Chajra,Thibaut Saguet,Corinne Granger,Lionel Breton,Pedro Contreiras Pinto,Mickael Machicoane,Jean Marc Le Doussal

Pharmaceutics 15: PubMed36678869

2023

A Novel (L.) Britton Cell-Derived Phytocomplex Regulates Keratinocytes Inflammatory Cascade and Barrier Function and Preserves Vaginal Mucosal Integrity In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanna Pressi,Giovanna Rigillo,Paolo Governa,Vittoria Borgonetti,Giulia Baini,Raffaella Rizzi,Chiara Guarnerio,Oriana Bertaiola,Marco Frigo,Matilde Merlin,Stefania Paltrinieri,Roberto Zambonin,Stefano Pandolfo,Marco Biagi

Biomedicines 10: PubMed35625870

2022

Significantly Altered Serum Levels of NAD, AGE, RAGE, CRP, and Elastin as Potential Biomarkers of Psoriasis and Aging-A Case-Control Study.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Karas,Drahomira Holmannova,Pavel Borsky,Zdenek Fiala,Ctirad Andrys,Kvetoslava Hamakova,Tereza Svadlakova,Vladimir Palicka,Jan Krejsek,Vit Rehacek,Monika Esterkova,Helena Kovarikova,Lenka Borska

ACS omega 4:22638-22645 PubMed31909348

2019

Preparation of Engineered Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells with Ultrasonication for Skin Rejuvenation.

Applications

Unspecified application

Species

Unspecified reactive species

Lixue Wang,Komal K Abhange,Yi Wen,Yundi Chen,Fei Xue,Guosheng Wang,Jinlong Tong,Chuandong Zhu,Xia He,Yuan Wan
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com