Human Elastin ELISA Kit

Product details

Human Elastin ELISA Kit ab239433 is a rapid single-wash 90-min ELISA kit to measure Human Elastin in serum and plasma. This SimpleStep ELISA® sensitivity is 44 pg/mL.

How the assay works

Human Elastin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit is designed for the quantification of human Elastin. The standard protein in this kit is an N-terminal fragment representing approximately 40% of the full-length mass of mature human Elastin. Native signal was detected in serum, plasma (citrate), and plasma (EDTA) sample types. Spiked protein experiments were used to validate serum, plasma (citrate), and plasma (EDTA) sample types. Cell culture supernatant, saliva, urine, milk, CSF, cell extract, and tissue extract samples have not been tested with this kit. This kit is incompatible with plasma (heparin) samples.

Human Elastin ELISA Kit ab239433 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human Elastin ELISA Kit ab239433

Human Elastin ELISA Kit ab239433 has been used to study biomarkers of psoriasis and aging ⁽¹⁾and skin rejuvenation using engineered extracellular vesicles⁽²⁾.

References:

⁽¹⁾Lenka Borska et al. 2022, PMID: 35625870,
⁽²⁾Yuan Wan et al. 2019, PMID: 31909348

Save your precious samples

Our 384-well Human Elastin ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Human Elastin Antibody Pair - BSA and Azide free ab256604
- Anti-Elastin antibody [EPR20414-6] - BSA and Azide free (Capture) ab259455
- Anti-Elastin antibody [EPR20414-34] - BSA and Azide free (Detector) ab259456

Related and recommended products

Human Elastin ELISA Kit ab239433 is commonly used to study tissue structure and function. Elastin is a component of the extracellular matrix (ECM).

Other ELISA kits to measure the ECM and its role in tissue function include:

- Human Collagen Type I ELISA kit ab285250
- Human Pro-Collagen I alpha 1 ELISA kit ab210966
- Total Glycosaminoglycans Assay Kit ab289842