Human EMMPRIN ELISA Kit, colorimetric, 90-min ELISA (ab219631)

Human EMMPRIN ELISA Kit is a single-wash 90-min Simplestep used to quantify Human EMMPRIN with a sensitivity of 3.88 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- KO validated for guaranteed specificity

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Key facts

Detection method: Colorimetric
Sample types: Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 31.3 - 200 pg/mL
Assay time: 1h 30m
Sensitivity: = 3.88 pg/mL

Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

See full target information BSG

Function

Isoform 1. Essential for normal retinal maturation and development (By similarity). Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors (PubMed:25957687). In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors (PubMed:25957687). May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes (PubMed:21620857). Isoform 1. (Microbial infection) Erythrocyte receptor for P.falciparum RH5 which is essential for erythrocyte invasion by the merozoite stage of P.falciparum isolates 3D7 and Dd2. Isoform 2. Signaling receptor for cyclophilins, essential for PPIA/CYPA and PPIB/CYPB-dependent signaling related to chemotaxis and adhesion of immune cells (PubMed:11688976, PubMed:11943775). Plays an important role in targeting monocarboxylate transporters SLC16A1/GLUT1, SLC16A11 and SLC16A12 to the plasma membrane (PubMed:17127621, PubMed:21778275, PubMed:28666119). Acts as a coreceptor for vascular endothelial growth factor receptor 2 (KDR/VEGFR2) in endothelial cells enhancing its VEGFA-mediated activation and downstream signaling (PubMed:25825981). Promotes angiogenesis through EPAS1/HIF2A-mediated up-regulation of VEGFA (isoform VEGF-165 and VEGF-121) and KDR/VEGFR2 in endothelial cells (PubMed:19837976). Plays a key role in regulating tumor growth, invasion, metastasis and neoangiogenesis by stimulating the production and release of extracellular matrix metalloproteinases and KDR/VEGFR2 by both tumor cells and stromal cells (fibroblasts and endothelial cells) (PubMed:11992541, PubMed:12553375, PubMed:15833850). Isoform 2. (Microbial infection) Erythrocyte receptor for P.falciparum RH5 which is essential for erythrocyte invasion by the merozoite stage of P.falciparum isolates 3D7, Dd2, 7G8 and HB3 (PubMed:22080952, PubMed:26195724). Binding of P.falciparum RH5 results in BSG dimerization which triggers an increase in intracellular Ca(2+) in the erythrocyte (PubMed:28409866). This essential step leads to a rearrangement of the erythrocyte cytoskeleton required for the merozoite invasion (PubMed:28409866). Isoform 2. (Microbial infection) Can facilitate human SARS coronavirus (SARS-CoV-1) infection via its interaction with virus-associated PPIA/CYPA. Isoform 2. (Microbial infection) Can facilitate HIV-1 infection via its interaction with virus-associated PPIA/CYPA. Isoform 2. (Microbial infection) First described as a receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is not required for SARS-CoV-2 infection. Isoform 2. (Microbial infection) Acts as a receptor for measles virus. Isoform 2. (Microbial infection) Promotes entry of pentamer-expressing human cytomegalovirus (HCMV) into epithelial and endothelial cells.

Alternative names

CD147, UNQ6505/PRO21383, BSG, Basigin, 5F7, Collagenase stimulatory factor, Extracellular matrix metalloproteinase inducer, Hepatoma-associated antigen, Leukocyte activation antigen M6, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor, EMMPRIN, HAb18G, TCSF

What's included?

1 x 96 Tests

Components

10X Human EMMPRIN Capture Antibody

1 x 600 µL

10X Human EMMPRIN Detector Antibody

1 x 600 µL

10X Wash Buffer PT (ab206977)

1 x 20 mL

Antibody Diluent 4BI

1 x 6 mL

Human EMMPRIN Lyophilized Recombinant Protein

2 x 1 Vial

Plate Seals

1 x 1 Unit

Sample Diluent 50BS

1 x 20 mL

Sample Diluent NS (ab193972)

1 x 50 mL

SimpleStep Pre-Coated 96-Well Microplate (ab206978)

1 x 1 Unit

Stop Solution

1 x 12 mL

TMB Development Solution

1 x 12 mL

Key facts

Detection method: Colorimetric
Sample types: Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 31.3 - 200 pg/mL
Assay time: 1h 30m
Assay Platform: Pre-coated microplate (12 x 8 well strips)
Sensitivity: = 3.88 pg/mL

Precision

Intra assay

Sample	n	C.V.
Sample Urine	n 8	C.V. 2.5

Inter assay

Sample	n	C.V.
Sample Urine	n 3	C.V. 3

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Cell culture supernatant	Average % = 112	Range 111 - 113 %
Sample type Serum	Average % = 97	Range 91 - 102 %
Sample type EDTA Plasma	Average % = 108	Range 107 - 109 %
Sample type Saliva	Average % = 95	Range 94 - 95 %
Sample type Urine	Average % = 98	Range 97 - 98 %
Sample type Heparin Plasma	Average % = 115	Range 113 - 117 %
Sample type Citrate plasma	Average % = 102	Range 100 - 105 %
Sample type Cell culture media	Average % = 103	Range 99 - 110 %

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

Human EMMPRIN ELISA Kit (ab219631) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of EMMPRIN protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, saliva, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human EMMPRIN with 3.88 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Extracellular matrix metalloproteinase inducer (EMMPRIN), also known as Basigin (BSG), and CD147 is a 363-amino acid protein encoded by the gene BSG. EMMPRIN is a member of the immunoglobin superfamily, and targets the monocarboxylate transporters SLC16A1, SLC16A3, and SLC16A8 to the plasma membrane. EMMPRIN is the determinant for the Oκ blood group system, and has been demonstrated to be a receptor on red blood cells for the malaria parasite, Plasmodium falciparum. In addition to its metalloproteinase-inducing ability, EMMPRIN is thought to regulate several other functions, including spermatogenesis, embryo implantation, neural network formation, and tumor progression. EMMPRIN also has several ligands including cyclophilin proteins, caveolin-1, and integrins.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD147 also referred to as EMMPRIN or basigin is a transmembrane glycoprotein with a molecular weight of roughly 50–60 kDa. This protein is found on the surface of many cell types including leukocytes platelets and endothelial cells and it plays a critical role in cell function related to immune responses and cellular interactions. CD147 interacts with several cellular components serving mechanical functions such as facilitating cell-to-cell communication and contributing to the stability of cell structures. The protein is ubiquitously expressed but exhibits higher levels in tissues like the brain skin and liver.

Biological function summary

CD147 engages in multiple roles beyond its mechanical functions. It functions as a part of protein complexes and is involved in processes such as regulation of matrix metalloproteinases (MMPs) which are pivotal in tissue remodeling and repair. It can modulate inflammatory responses and is essential for cellular signaling pathways that affect cellular metabolism and growth. The involvement of CD147 in immune responses indicates its importance in various physiological processes.

Pathways

CD147 plays a significant role in the MAPK and NF-kB signaling pathways which are fundamental for controlling inflammatory responses and cell survival. It interacts with proteins such as integrins and cyclophilins which are important for mediating these pathways. These interactions highlight CD147's impact on cellular dynamics and its potential role in modulating the cellular environment in response to external stimuli.

Associated diseases and disorders

CD147 has been implicated in cancer promotion and progression as well as inflammatory diseases like rheumatoid arthritis. Its overexpression is often observed in tumors where it interacts with MMPs to facilitate tumor invasion and metastasis. In inflammatory conditions CD147 can influence the behavior of proteins like cytokines exacerbating symptoms and contributing to disease severity. These associations make CD147 a potential target for therapeutic intervention in cancer and inflammatory diseases.

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9 product images

Sandwich ELISA - Human EMMPRIN ELISA Kit (ab219631)
Example of human EMMPRIN standard curve in Sample Diluent 50BS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human EMMPRIN ELISA Kit (ab219631)
Example of human EMMPRIN standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human EMMPRIN ELISA Kit (ab219631)
Interpolated concentrations of spiked EMMPRIN in human serum and plasma samples.
The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 1,572 pg/mL in neat serum, 1,440 pg/mL in neat plasma (citrate), 1,516 pg/mL in neat plasma (EDTA), and 1,740 pg/mL in neat plasma (heparin).
Sandwich ELISA - Human EMMPRIN ELISA Kit (ab219631)
Interpolated concentrations of native EMMPRIN in human serum and plasma samples.
The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 634 pg/mL in neat serum, 531 pg/mL in neat plasma (citrate), 563 pg/mL in neat plasma (EDTA), and 599 pg/mL in neat plasma (heparin).
Sandwich ELISA - Human EMMPRIN ELISA Kit (ab219631)
Interpolated concentrations of native EMMPRIN in human urine and spiked EMMPRIN in human saliva and PBMC stimulated cultured media.
The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 50%, saliva 100%, and PBMC stimulated culture media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 3,245 pg/mL in neat urine, 1,135 pg/mL in neat saliva, and 1,111 pg/mL in neat PBMC stimulated culture media.
Sandwich ELISA - Human EMMPRIN ELISA Kit (ab219631)
Interpolated concentrations of native EMMPRIN in human saliva and PBMC stimulated cultured media.
The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 100% and PBMC stimulated culture media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 69 pg/mL in neat saliva, and 75 pg/mL in neat PBMC stimulated culture media.
Sandwich ELISA - Human EMMPRIN ELISA Kit (ab219631)
Interpolated concentrations of native EMMPRIN in human cell culture supernatant samples.
The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated PBMC media 100%, stimulated PBMC media 100%, untreated MCF-7 media 100%, and treated MCF-7 media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 72 pg/mL in neat stimulated PBMC media and 64 pg/mL in neat treated MCF-7 media. The unstimulated PBMC media and untreated MCF-7 media samples measured below the 7^th point of the standard curve. PBMC media samples were cultured in RPMI media with 10% fetal bovine serum (unstimulated) for 24 hours and then stimulated for 48 hours with 1.5% PHAM. MCF-7 media samples were cultured in DMEM media with 10% fetal bovine serum (untreated) and then treated with 0.12 mM NEAA with 1 mM sodium pyruvate (treated) for 24 hours.
Sandwich ELISA - Human EMMPRIN ELISA Kit (ab219631)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 625 pg/mL with a range of 445- 836 pg/mL.
Sandwich ELISA - Human EMMPRIN ELISA Kit (ab219631)
Human CD147 concentration was interpolated from the EMMPRIN (CD147) standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human EMMPRIN ELISA kit (ab219631). Wild-type and CD147 knockout A549 cells (Human BSG (CD147) knockout A549 cell line ab273748) were assessed in duplicate (n=2); Jurkat and Raji cells were used as positive and negative controls respectively (n=1). Where samples were run in duplicate, data are represented as the mean and error bars represent standard deviation.