Human EMMPRIN ELISA Kit
- KO Validated
- Recombinant
- SimpleStep
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(1 Publication)
Human EMMPRIN ELISA Kit is a single-wash 90-min Simplestep used to quantify Human EMMPRIN with a sensitivity of 3.88 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- KO validated for guaranteed specificity
View Alternative Names
CD147, UNQ6505/PRO21383, BSG, Basigin, 5F7, Collagenase stimulatory factor, Extracellular matrix metalloproteinase inducer, Hepatoma-associated antigen, Leukocyte activation antigen M6, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor, EMMPRIN, HAb18G, TCSF
- sELISA
Supplier Data
Sandwich ELISA - Human EMMPRIN ELISA Kit (AB219631)
Interpolated concentrations of native EMMPRIN in human urine and spiked EMMPRIN in human saliva and PBMC stimulated cultured media.
The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 50%, saliva 100%, and PBMC stimulated culture media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 3,245 pg/mL in neat urine, 1,135 pg/mL in neat saliva, and 1,111 pg/mL in neat PBMC stimulated culture media.
- sELISA
Supplier Data
Sandwich ELISA - Human EMMPRIN ELISA Kit (AB219631)
Interpolated concentrations of native EMMPRIN in human saliva and PBMC stimulated cultured media.
The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Undiluted samples are as follows : saliva 100% and PBMC stimulated culture media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 69 pg/mL in neat saliva, and 75 pg/mL in neat PBMC stimulated culture media.
- sELISA
Supplier Data
Sandwich ELISA - Human EMMPRIN ELISA Kit (AB219631)
Interpolated concentrations of native EMMPRIN in human cell culture supernatant samples.
The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Undiluted samples are as follows : unstimulated PBMC media 100%, stimulated PBMC media 100%, untreated MCF-7 media 100%, and treated MCF-7 media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 72 pg/mL in neat stimulated PBMC media and 64 pg/mL in neat treated MCF-7 media. The unstimulated PBMC media and untreated MCF-7 media samples measured below the 7th point of the standard curve. PBMC media samples were cultured in RPMI media with 10% fetal bovine serum (unstimulated) for 24 hours and then stimulated for 48 hours with 1.5% PHAM. MCF-7 media samples were cultured in DMEM media with 10% fetal bovine serum (untreated) and then treated with 0.12 mM NEAA with 1 mM sodium pyruvate (treated) for 24 hours.
- sELISA
Lab
Sandwich ELISA - Human EMMPRIN ELISA Kit (AB219631)
Human CD147 concentration was interpolated from the EMMPRIN (CD147) standard curve. Supernatants from cell culture samples were serially diluted and assessed by the Human EMMPRIN ELISA kit (ab219631). Wild-type and CD147 knockout A549 cells (ab273748) were assessed in duplicate (n=2); Jurkat and Raji cells were used as positive and negative controls respectively (n=1). Where samples were run in duplicate, data are represented as the mean and error bars represent standard deviation.
- sELISA
Supplier Data
Sandwich ELISA - Human EMMPRIN ELISA Kit (AB219631)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 625 pg/mL with a range of 445- 836 pg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human EMMPRIN ELISA Kit (AB219631)
Interpolated concentrations of native EMMPRIN in human serum and plasma samples.
The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 634 pg/mL in neat serum, 531 pg/mL in neat plasma (citrate), 563 pg/mL in neat plasma (EDTA), and 599 pg/mL in neat plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human EMMPRIN ELISA Kit (AB219631)
Interpolated concentrations of spiked EMMPRIN in human serum and plasma samples.
The concentrations of EMMPRIN were measured in duplicates, interpolated from the EMMPRIN standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EMMPRIN concentration was determined to be 1,572 pg/mL in neat serum, 1,440 pg/mL in neat plasma (citrate), 1,516 pg/mL in neat plasma (EDTA), and 1,740 pg/mL in neat plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human EMMPRIN ELISA Kit (AB219631)
Example of human EMMPRIN standard curve in Sample Diluent 50BS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human EMMPRIN ELISA Kit (AB219631)
Example of human EMMPRIN standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Human EMMPRIN ELISA Kit (ab219631) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of EMMPRIN protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, saliva, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human EMMPRIN with 3.88 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Extracellular matrix metalloproteinase inducer (EMMPRIN), also known as Basigin (BSG), and CD147 is a 363-amino acid protein encoded by the gene BSG. EMMPRIN is a member of the immunoglobin superfamily, and targets the monocarboxylate transporters SLC16A1, SLC16A3, and SLC16A8 to the plasma membrane. EMMPRIN is the determinant for the Oκ blood group system, and has been demonstrated to be a receptor on red blood cells for the malaria parasite, Plasmodium falciparum. In addition to its metalloproteinase-inducing ability, EMMPRIN is thought to regulate several other functions, including spermatogenesis, embryo implantation, neural network formation, and tumor progression. EMMPRIN also has several ligands including cyclophilin proteins, caveolin-1, and integrins.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD147 engages in multiple roles beyond its mechanical functions. It functions as a part of protein complexes and is involved in processes such as regulation of matrix metalloproteinases (MMPs) which are pivotal in tissue remodeling and repair. It can modulate inflammatory responses and is essential for cellular signaling pathways that affect cellular metabolism and growth. The involvement of CD147 in immune responses indicates its importance in various physiological processes.
Pathways
CD147 plays a significant role in the MAPK and NF-kB signaling pathways which are fundamental for controlling inflammatory responses and cell survival. It interacts with proteins such as integrins and cyclophilins which are important for mediating these pathways. These interactions highlight CD147's impact on cellular dynamics and its potential role in modulating the cellular environment in response to external stimuli.
Product protocols
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Target data
Publications (1)
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Science advances 6:eaaz6119 PubMed32637597
2020
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