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AB313688

Human EMR2 ELISA Kit

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Human EMR2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human EMR2 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

CD312, EMR2, ADGRE2, Adhesion G protein-coupled receptor E2, EGF-like module receptor 2, EGF-like module-containing mucin-like hormone receptor-like 2

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Sandwich ELISA - Human EMR2 ELISA Kit (AB313688)
  • sELISA

Supplier Data

Sandwich ELISA - Human EMR2 ELISA Kit (AB313688)

Example of standard curve using ab313688. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 18 pg/mL

Range

18 - 4000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human EMR2 (EGF-like module-containing mucin-like receptor 2) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human EMR2 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human EMR2 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "75 - 96 %", "average": "= 86.42" }, { "sample": "Plasma", "range": "73 - 106 %", "average": "= 88.5" }, { "sample": "Cell culture media", "range": "76 - 123 %", "average": "= 99.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313688-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Biotinylated Human EMR2 Detection Antibody", "number":"AB313688-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin", "number":"AB313688-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313688-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313688-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human EMR2 Antibody-coated ELISA Plate", "number":"AB313688-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human EMR2 Protein Standard", "number":"AB313688-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313688-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313688-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EMR2 also known as epidermal growth factor-like module receptor 2 or CD312 is a member of the class B G protein-coupled receptor family. It is a large receptor with an approximate mass of 220 kDa. EMR2 is mainly expressed on leukocytes especially myeloid cells like monocytes and macrophages. EMR2 consists of an extensive extracellular domain that is involved in ligand binding influencing cell adhesion and signaling processes.
Biological function summary

EMR2 plays a role in the immune system by facilitating cell-cell interactions and guiding leukocyte trafficking. It forms part of a receptor complex that includes other G protein-coupled receptors. The receptor is essential in the regulation of inflammatory responses helping in the recognition and response to pathogens or tissue damage. EMR2's function contributes to the activation and migration of immune cells to sites of infection or injury.

Pathways

EMR2 interacts with intracellular signaling cascades such as the MAPK and PI3K pathways that manage cell proliferation survival and immune responses. Through these pathways EMR2 connects to proteins such as Akt and ERK facilitating downstream signaling mechanisms important for immune cell activation. The integration of EMR2 in these pathways positions it as an important player in cellular responses to external stimuli.

Aberrations in EMR2 function or expression relate to chronic inflammatory conditions and autoimmune disorders. Conditions such as rheumatoid arthritis and inflammatory bowel disease show altered EMR2 signaling indicating its role in the pathology of these diseases. In the context of these disorders other proteins like TNF-alpha and IL-6 often interact with EMR2 linking it to pro-inflammatory pathways and the exacerbation of disease symptoms.
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Product protocols

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Target data

Cell surface receptor that binds to the chondroitin sulfate moiety of glycosaminoglycan chains and promotes cell attachment. Promotes granulocyte chemotaxis, degranulation and adhesion. In macrophages, promotes the release of inflammatory cytokines, including IL8 and TNF. Signals probably through G-proteins. Is a regulator of mast cell degranulation (PubMed : 26841242).
See full target information ADGRE2
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websiteProtocolBooklet
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Product promise

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