AB100508

Human Endostatin ELISA Kit

Name: Human Endostatin ELISA Kit (ab100508) | Abcam
Brand: Abcam Limited
SKU: AB100508
Price: 780 USD
Availability: InStock

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(5 Publications)

Human Endostatin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Endostatin in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Collagen alpha-1(XVIII) chain, COL18A1

2 Images

Sandwich ELISA - Human Endostatin ELISA Kit (AB100508)

sELISA

Unknown

Sandwich ELISA - Human Endostatin ELISA Kit (AB100508)

Representative Standard Curve using ab100508

sELISA

Supplier Data

Sandwich ELISA - Human Endostatin ELISA Kit (AB100508)

Representative Standard Curve using ab100508

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

13.7 - 10000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's Human Endostatin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Endostatin in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human Endostatin coated on a 96-well plate. Standards and samples are pipetted into the wells and Endostatin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Endostatin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Endostatin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get results in 90 minutes with Human Endostatin ELISA Kit (ab184864) from our SimpleStep ELISA^® range.

Optimisation may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "84 - 108 %", "average": "= 95.32" }, { "sample": "Serum", "range": "78 - 102 %", "average": "= 88.47" }, { "sample": "Plasma", "range": "80 - 104 %", "average": "= 92.31" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100508-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100508-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Endostatin Standard Human(recombinant) (lyophilized)", "number":"AB100508-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Endostatin Microplate (12 strips x 8 wells)", "number":"AB100508-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100508-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100508-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Detection Antibody Endostatin", "number":"AB100508-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100508-CMP01", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100508-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100508-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Endostatin also known as Collagen XVIII alpha 1 (COL18A1) is a powerful anti-angiogenic protein with a mass around 20 kDa. It specifically inhibits the process of blood vessel formation in both in vitro and in vivo environments. This protein derives as a fragment from the carboxyl end of collagen XVIII which is a component of basement membranes. Expression of endostatin occurs primarily in the endothelial cells lining blood vessels and is also seen in tissues undergoing remodeling or repair.

Biological function summary

Endostatin functions as an important inhibitor of angiogenesis the process in which new blood vessels form from pre-existing ones. It acts independently and is not part of a larger protein complex. In the cellular microenvironment endostatin binds to various integrins inhibiting endothelial cell proliferation and inducing apoptosis. It also disrupts signal transduction pathways critical for endothelial cell survival and migration.

Pathways

Endostatin holds an important position in the angiogenesis and Wnt signaling pathways. It interacts closely with proteins like VEGF (Vascular Endothelial Growth Factor) and FGF2 (Fibroblast Growth Factor 2) where it counteracts their pro-angiogenic effects. Endostatin's role in these pathways indicates its significant impact on controlling angiogenic balance in various physiological and pathological conditions.

Endostatin exhibits notable relevance to cancer and diabetic retinopathy. In cancer the protein suppresses tumor-induced angiogenesis restricting tumor growth and metastasis. Likewise in diabetic retinopathy endostatin's inhibition of abnormal blood vessel formation in the retina offers potential therapeutic benefits. In these contexts it interacts with other key proteins like MMPs (matrix metalloproteinases) which regulate collagen degradation and release of endostatin fragments from parent collagen XVIII.

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Product protocols

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Target data

Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.. Non-collagenous domain 1. May regulate extracellular matrix-dependent motility and morphogenesis of endothelial and non-endothelial cells; the function requires homotrimerization and implicates MAPK signaling.. Endostatin. Potently inhibits endothelial cell proliferation and angiogenesis (PubMed : 9459295). May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling (By similarity). Inhibits VEGFA-induced endothelial cell proliferation and migration. Seems to inhibit VEGFA-mediated signaling by blocking the interaction of VEGFA to its receptor KDR/VEGFR2. Modulates endothelial cell migration in an integrin-dependent manner implicating integrin ITGA5 : ITGB1 and to a lesser extent ITGAV : ITGB3 and ITGAV : ITGB5 (By similarity). May negatively regulate the activity of homotrimeric non-collagenous domain 1 (PubMed : 11257123).

See full target information COL18A1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:21843 PubMed38071215

2023

Analysis of endothelial progenitor cell subtypes as clinical biomarkers for elderly patients with ischaemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Rais Reskiawan A Kadir,Kamini Rakkar,Othman A Othman,Nikola Sprigg,Philip M Bath,Ulvi Bayraktutan

Brain research 1787:147925 PubMed35469846

2022

Inhibition of oxidative stress delays senescence and augments functional capacity of endothelial progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rais Reskiawan A Kadir,Mansour Alwjwaj,Othman Ahmad Othman,Kamini Rakkar,Nikola Sprigg,Philip M Bath,Ulvi Bayraktutan

Microbiology spectrum 9:e0036421 PubMed34479416

2021

Prognostic Performance of Peripheral Blood Biomarkers in Identifying Seropositive Individuals at Risk of Developing Clinically Symptomatic Chagas Cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Subhadip Choudhuri,Suresh K Bhavnani,Weibin Zhang,Valentina Botelli,Natalia Barrientos,Facundo Iñiguez,Maria Paola Zago,Nisha Jain Garg

The Journal of international medical research 48:300060520940856 PubMed32691651

2020

Prognostic value of dynamic plasma endostatin for the prediction of mortality in acute kidney injury: A prospective cohort study.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Miao Jia,Yue Zheng,Yue Han,Wen-Liang Ma,Yi-Jia Jiang,Xi Zheng,Shu-Yan Guo,Tian-En Zhang,Wen-Xiong Li

Critical care (London, England) 22:305 PubMed30445971

2018

Derivation and validation of plasma endostatin for predicting renal recovery from acute kidney injury: a prospective validation study.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Miao Jia,Yue Zheng,Li-Feng Huang,Xin Xin,Wen-Liang Ma,Yi-Jia Jiang,Xi Zheng,Shu-Yan Guo,Wen-Xiong Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Endostatin ELISA Kit

Sandwich ELISA - Human Endostatin ELISA Kit (AB100508)

Sandwich ELISA - Human Endostatin ELISA Kit (AB100508)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay Platform

Reactivity data

Product details

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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