AB184864

Human Endostatin ELISA Kit

SimpleStep
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Human Endostatin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Endostatin with a sensitivity of 7.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

Collagen alpha-1(XVIII) chain, COL18A1

4 Images

Sandwich ELISA - Human Endostatin ELISA Kit (AB184864)

sELISA

Supplier Data

Sandwich ELISA - Human Endostatin ELISA Kit (AB184864)

Titration of normal Human serum and plasma samples to within working range of the assay. Background subtracted data from duplicate measurements are plotted.

sELISA

Supplier Data

Sandwich ELISA - Human Endostatin ELISA Kit (AB184864)

Titration of individual donor serum samples to within working range of the assay. Quantification of Endostatin interpolated from standard curve and multiplied by dilution factor.

sELISA

Supplier Data

Sandwich ELISA - Human Endostatin ELISA Kit (AB184864)

Titration of normal Human serum and plasma samples to within working range of the assay. Bar graph denotes quantification of Endostatin interpolated from standard curve and multiplied by dilution factor.

sELISA

Supplier Data

Sandwich ELISA - Human Endostatin ELISA Kit (AB184864)

Example of Endostatin standard curve. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 7.7 pg/mL

Range

32 - 2000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Endostatin ELISA kit (ab184864) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Endostatin protein in human serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Endostatin with 7.7 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

Endostatin (20-22 kDa), a cleaved product of the C-terminal domain of collagen XVIII, is an endogenous angiogenesis inhibitor. It was originally found in conditioned media from a murine endothelial tumor cell line, hemangioendothelioma. Endostatin inhibits endothelial cell migration (in vivo and in vitro) and induces endothelial cell apoptosis. It inhibits tumor growth and impairs blood vessel maturation in wound healing. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.9" } ]

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Recovery

[ { "sample": "Serum", "range": "99 - 114 %", "average": "= 106" }, { "sample": "Plasma", "range": "104 - 107 %", "average": "= 105" }, { "sample": "Cell culture media", "range": "87 - 88 %", "average": "= 88" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab184864-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB184864-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB184864-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Endostatin Capture Antibody", "number":"AB184864-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB184864-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Endostatin Lyophilized Recombinant Protein", "number":"AB184864-CMP02", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB184864-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB184864-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB184864-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB184864-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Endostatin Detector Antibody", "number":"AB184864-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Endostatin also known as Collagen XVIII alpha 1 (COL18A1) is a powerful anti-angiogenic protein with a mass around 20 kDa. It specifically inhibits the process of blood vessel formation in both in vitro and in vivo environments. This protein derives as a fragment from the carboxyl end of collagen XVIII which is a component of basement membranes. Expression of endostatin occurs primarily in the endothelial cells lining blood vessels and is also seen in tissues undergoing remodeling or repair.

Biological function summary

Endostatin functions as an important inhibitor of angiogenesis the process in which new blood vessels form from pre-existing ones. It acts independently and is not part of a larger protein complex. In the cellular microenvironment endostatin binds to various integrins inhibiting endothelial cell proliferation and inducing apoptosis. It also disrupts signal transduction pathways critical for endothelial cell survival and migration.

Pathways

Endostatin holds an important position in the angiogenesis and Wnt signaling pathways. It interacts closely with proteins like VEGF (Vascular Endothelial Growth Factor) and FGF2 (Fibroblast Growth Factor 2) where it counteracts their pro-angiogenic effects. Endostatin's role in these pathways indicates its significant impact on controlling angiogenic balance in various physiological and pathological conditions.

Endostatin exhibits notable relevance to cancer and diabetic retinopathy. In cancer the protein suppresses tumor-induced angiogenesis restricting tumor growth and metastasis. Likewise in diabetic retinopathy endostatin's inhibition of abnormal blood vessel formation in the retina offers potential therapeutic benefits. In these contexts it interacts with other key proteins like MMPs (matrix metalloproteinases) which regulate collagen degradation and release of endostatin fragments from parent collagen XVIII.

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Product protocols

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Target data

Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.. Non-collagenous domain 1. May regulate extracellular matrix-dependent motility and morphogenesis of endothelial and non-endothelial cells; the function requires homotrimerization and implicates MAPK signaling.. Endostatin. Potently inhibits endothelial cell proliferation and angiogenesis (PubMed : 9459295). May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling (By similarity). Inhibits VEGFA-induced endothelial cell proliferation and migration. Seems to inhibit VEGFA-mediated signaling by blocking the interaction of VEGFA to its receptor KDR/VEGFR2. Modulates endothelial cell migration in an integrin-dependent manner implicating integrin ITGA5 : ITGB1 and to a lesser extent ITGAV : ITGB3 and ITGAV : ITGB5 (By similarity). May negatively regulate the activity of homotrimeric non-collagenous domain 1 (PubMed : 11257123).

See full target information COL18A1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human Endostatin ELISA Kit

Sandwich ELISA - Human Endostatin ELISA Kit (AB184864)

Sandwich ELISA - Human Endostatin ELISA Kit (AB184864)

Sandwich ELISA - Human Endostatin ELISA Kit (AB184864)

Sandwich ELISA - Human Endostatin ELISA Kit (AB184864)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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