AB181417

Human ENO1 ELISA Kit (Alpha-Enolase)

SimpleStep
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Human ENO1 ELISA Kit (Alpha-Enolase) is a single-wash 90-min Simplestep used to quantify Human ENO1 (Alpha-Enolase) with a sensitivity of 6.5 U/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

ENO1L1, MBPB1, MPB1, ENO1, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, NNE

5 Images

ELISA - Human ENO1 ELISA Kit (Alpha-Enolase) (AB181417)

ELISA

Supplier Data

ELISA - Human ENO1 ELISA Kit (Alpha-Enolase) (AB181417)

sELISA

Supplier Data

Sandwich ELISA - Human ENO1 ELISA Kit (Alpha-Enolase) (AB181417)

Human recombinant ENO1 protein (600 ng/mL) and neuron-specific human recombinant ENO2 protein (600 ng/mL) were analyzed with this kit

sELISA

Supplier Data

Sandwich ELISA - Human ENO1 ELISA Kit (Alpha-Enolase) (AB181417)

Interpolated concentrations of native ENO1 in human HeLa cell extract based on a 50 µg/mL extract load and human liver tissue extract based on a 286 μg/mL extract load

sELISA

Supplier Data

Sandwich ELISA - Human ENO1 ELISA Kit (Alpha-Enolase) (AB181417)

Extracts of human liver and human heart were analyzed with this kit

sELISA

Supplier Data

Sandwich ELISA - Human ENO1 ELISA Kit (Alpha-Enolase) (AB181417)

Example of human ENO1 standard curve in 1X Cell Extraction Buffer PTR

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Serum, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6.5 U/mL

Range

27.85 - 800 U/mL

Assay time

1h 30m

Assay Platform

Microplate

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human ENO1 ELISA Kit (Alpha-Enolase) ab181417 is a rapid single-wash 90-min Sandwich ELISA to measure Human ENO1 (Alpha-Enolase)in cell culture extracts, serum, tissue extracts. This SimpleStep sensitivity is 6.5 0.

How the assay works

Human ENO1 (Alpha-Enolase)SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human ENO1 ELISA Kit (Alpha-Enolase) ab181417 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Alpha enolase (ENO1, P06733) ENO1 is localized to cytoplasm. It can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. MBP1, a shorter isoform of the ENO1, binds to the myc promoter and acts as a transcriptional repressor. It may be a tumor suppressor. It locates mainly in nucleus. ENO1 is used as a diagnostic marker for many tumors and, in the heterodimeric form, alpha/gamma, as a marker for hypoxic brain injury after cardiac arrest. Also it is a marker for endometriosis. Antibodies against ENO1 are present in sera from patients with cancer-associated retinopathy syndrome (CAR), a progressive blinding disease which occurs in the presence of systemic tumor growth, primarily small-cell carcinoma of the lung and other malignancies. ENO1 is identified as an autoantigen in Hashimoto encephalopathy (HE) a rare autoimmune disease associated with Hashimoto thyroiditis (HT). HT is a disorder in which destructive processes overcome the potential capacity of thyroid replacement leading to hypothyroidism.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "HeLa Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.4" }, { "reproducibilityType": "Intra", "sample": "HeLa Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "100 - 103 %", "average": "= 101" }, { "sample": "Cell culture extracts", "range": "92 - 93 %", "average": "= 93" }, { "sample": "Tissue Extracts", "range": "99 - 113 %", "average": "= 104" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab181417-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB181417-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB181417-CMP13", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB181417-CMP14", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB181417-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ENO1 Capture Antibody", "number":"AB181417-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB181417-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB181417-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB181417-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human ENO1 Lyophilized Recombinant Protein", "number":"AB181417-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ENO1 Detector Antibody", "number":"AB181417-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB181417-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB181417-CMP11", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Enolase 1 or alpha-enolase is an important enzyme in the glycolytic pathway. It catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. We also refer to it as an enolase reaction. ENO1 protein has a molecular mass of approximately 47 kDa and is expressed in most tissues but found most abundantly in the brain muscle and liver. Additionally ENO1 can exist in both cytosolic and plasma membrane-associated forms playing functional roles in different cellular contexts.

Biological function summary

The ENO1 protein serves multiple functions beyond its glycolytic activity. It also acts as a plasminogen receptor on the cell surface aiding in the modulation of pericellular and extracellular matrix degradation processes. Through this role ENO1 influences cell migration and tissue remodeling. This protein can form part of larger protein complexes contributing to its versatility in various cellular functions.

Pathways

ENO1 plays a critical role in glycolysis a central metabolic pathway that converts glucose into pyruvate generating ATP as a result. In this pathway ENO1 closely interacts with other enzymes like phosphoglycerate kinase and pyruvate kinase. Additionally as a plasminogen receptor ENO1 links to the plasminogen activation pathway affecting fibrinolysis and cellular response to tissue injury.

ENO1 is associated with cancer and neurodegenerative diseases. Overexpression or aberrant regulation of ENO1 often correlates with tumor progression and metastasis in cancers such as lung and breast cancer. In neurodegenerative disorders like Alzheimer's disease abnormal functioning of ENO1 can impact brain energy metabolism and neuron degeneration. ENO1's interaction with other proteins such as beta-enolase further connects it to these conditions influencing disease mechanisms and patient outcomes.

style

left-column

Product protocols

style

left-column

Target data

Enolase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis (PubMed : 1369209, PubMed : 29775581). Also involved in various processes such as growth control, hypoxia tolerance and allergic responses (PubMed : 10802057, PubMed : 12666133, PubMed : 2005901, PubMed : 29775581). May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (PubMed : 12666133). Stimulates immunoglobulin production (PubMed : 1369209).. Isoform MBP-1. Binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.

See full target information ENO1

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of oral & facial pain and headache 38:111-118 PubMed39801101

2025

Changes in salivary biomarkers of burning mouth syndrome patients after clonazepam treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Sungil Jang,Ji-Eun Kim,Young-Hee Lee,Won Jung

Stroke and vascular neurology 10:347-358 PubMed39357895

2024

Extracellular vesicles bearing serum amyloid A1 exacerbate neuroinflammation after intracerebral haemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Zhu,Ningning Wang,Yingying Chang,Ying Zhang,Shihe Jiang,Xiaoping Ren,Meng Yuan,Haoxiao Chang,Wei-Na Jin

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human ENO1 ELISA Kit (Alpha-Enolase)

ELISA - Human ENO1 ELISA Kit (Alpha-Enolase) (AB181417)

Sandwich ELISA - Human ENO1 ELISA Kit (Alpha-Enolase) (AB181417)

Sandwich ELISA - Human ENO1 ELISA Kit (Alpha-Enolase) (AB181417)

Sandwich ELISA - Human ENO1 ELISA Kit (Alpha-Enolase) (AB181417)

Sandwich ELISA - Human ENO1 ELISA Kit (Alpha-Enolase) (AB181417)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Primary Antibodies

Assay Kits

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise