Human ENPP2/ATX ELISA Kit (ab322364)

Human ENPP2/ATX ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human ENPP2/ATX in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine samples.

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Sandwich ELISA - Human ENPP2/ATX ELISA Kit (AB322364), expandable thumbnail

Key facts

Detection method: Colorimetric
Sample types: Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 125 - 8000 pg/mL
Assay time: 1h 30m
Sensitivity: = 45.91 pg/mL

Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

Target data

See full target information ENPP2

Function

Secreted lysophospholipase D that hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:12354767, PubMed:14500380, PubMed:15769751, PubMed:26371182, PubMed:27754931). Its major substrate is lysophosphatidylcholine (PubMed:12176993, PubMed:14500380, PubMed:27754931). Can also act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility (PubMed:14500380). Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP (PubMed:12176993, PubMed:15769751). Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation (PubMed:11559573). Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein (PubMed:1733949). May have a role in induction of parturition (PubMed:12176993). Possible involvement in cell proliferation and adipose tissue development (Probable). Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids (PubMed:21393252). Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) (PubMed:21393252).

Alternative names

ATX, PDNP2, ENPP2, Autotaxin, Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, Extracellular lysophospholipase D, E-NPP 2, LysoPLD

What's included?

10 x 96 Tests

Components

10X Human ENPP2/ATX Detector Antibody

10 x 600 µL

10X Wash Buffer PT (ab206977)

1 x 200 mL

Antibody Diluent 4BI

10 x 6 mL

Human ENPP2/ATX Lyophilized Recombinant Protein

20 x 1 Vial

Lyophilized Human ENPP2/ATX Capture Antibody

10 x 1 Vial

Plate Seal

10 x 1 Unit

Sample Diluent NS (ab193972)

2 x 250 mL

SimpleStep Pre-Coated 96-Well Microplate (ab206978)

10 x 1 Unit

Stop Solution

1 x 120 mL

TMB Development Solution

1 x 120 mL

Key facts

Detection method: Colorimetric
Sample types: Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 125 - 8000 pg/mL
Assay time: 1h 30m
Assay Platform: Microplate (12 x 8 well strips)
Sensitivity: = 45.91 pg/mL
General recovery: 102%

Precision

Intra assay

Sample	n	mean	SD	C.V.
Sample Serum	n 8	mean -	SD -	C.V. = 4

Inter assay

Sample	n	mean	SD	C.V.
Sample Serum	n 3	mean -	SD -	C.V. = 5.7

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Serum	Average % = 102	Range 99 - 105 %
Sample type Citrate plasma	Average % = 98	Range 98 - 99 %
Sample type EDTA Plasma	Average % = 99	Range 98 - 100 %
Sample type Heparin Plasma	Average % = 99	Range 97 - 99 %
Sample type Cell culture supernatant	Average % = 99	Range 98 - 100 %
Sample type Urine	Average % = 89	Range 88 - 91 %
Sample type Milk	Average % = 101	Range 100 - 103 %

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

Human ENPP2/ATX SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ENPP2/ATX protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant, Urine, Milk. Quantitate Human ENPP2/ATX with 45.91 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples -96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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9 product images

Sandwich ELISA - Human ENPP2/ATX ELISA Kit (ab322364)
Interpolated concentration of native ENPP2/ATX was measured in duplicate at different sample concentrations. Undiluted samples are as follows: Human serum 1:100 and Rhesus macaque monkey serum 1:200. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human ENPP2/ATX ELISA Kit (ab322364)
Standard curve comparison between Human ENPP2/ATX SimpleStep ELISA kit and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human ENPP2/ATX ELISA Kit (ab322364)
Interpolated concentration of native ENPP2/ATX was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:100 human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human ENPP2/ATX ELISA Kit (ab322364)
Example of human ENPP2/ATX standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human ENPP2/ATX ELISA Kit (ab322364)
Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean ENPP2/ATX concentration was determined to be 540 ng/mL with a range of 352 – 761 ng/mL.
Sandwich ELISA - Human ENPP2/ATX ELISA Kit (ab322364)
Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean ENPP2/ATX concentration was determined to be 808 ng/mL with a range of 426 – 1,501 ng/mL.
Sandwich ELISA - Human ENPP2/ATX ELISA Kit (ab322364)
Interpolated concentration of native ENPP2/ATX was measured in duplicate at different sample concentrations. Undiluted samples are as follows: U-87 MG cell culture supernatant 50% and milk 12.5%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human ENPP2/ATX ELISA Kit (ab322364)
Interpolated concentration of native ENPP2/ATX was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 1:100, plasma (citrate) 1:50, plasma (EDTA) 1:50, and plasma (heparin) 1:100. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human ENPP2/ATX ELISA Kit (ab322364)
Example of human ENPP2/ATX standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.