AB322364

Human ENPP2/ATX ELISA Kit

Recombinant
SimpleStep
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Human ENPP2/ATX ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ENPP2/ATX with a sensitivity of 45.91 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

9 Images

Sandwich ELISA - Human ENPP2/ATX ELISA Kit (AB322364)

sELISA

Supplier Data

Sandwich ELISA - Human ENPP2/ATX ELISA Kit (AB322364)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean ENPP2/ATX concentration was determined to be 808 ng/mL with a range of 426 – 1,501 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human ENPP2/ATX ELISA Kit (AB322364)

Interpolated concentration of native ENPP2/ATX was measured in duplicate at different sample concentrations. Undiluted samples are as follows : U-87 MG cell culture supernatant 50% and milk 12.5%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human ENPP2/ATX ELISA Kit (AB322364)

Interpolated concentration of native ENPP2/ATX was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Human serum 1 : 100 and Rhesus macaque monkey serum 1 : 200. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human ENPP2/ATX ELISA Kit (AB322364)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean ENPP2/ATX concentration was determined to be 540 ng/mL with a range of 352 – 761 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human ENPP2/ATX ELISA Kit (AB322364)

Interpolated concentration of native ENPP2/ATX was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 100, plasma (citrate) 1 : 50, plasma (EDTA) 1 : 50, and plasma (heparin) 1 : 100. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human ENPP2/ATX ELISA Kit (AB322364)

Interpolated concentration of native ENPP2/ATX was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 100 human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human ENPP2/ATX ELISA Kit (AB322364)

Example of human ENPP2/ATX standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human ENPP2/ATX ELISA Kit (AB322364)

Standard curve comparison between Human ENPP2/ATX SimpleStep ELISA kit and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human ENPP2/ATX ELISA Kit (AB322364)

Example of human ENPP2/ATX standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 45.91 pg/mL

Range

125 - 8000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human ENPP2/ATX SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ENPP2/ATX protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant, Urine, Milk. Quantitate Human ENPP2/ATX with 45.91 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples -96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.7" } ]

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Recovery

[ { "sample": "Serum", "range": "99 - 105 %", "average": "= 102" }, { "sample": "Citrate plasma", "range": "98 - 99 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "98 - 100 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "97 - 99 %", "average": "= 99" }, { "sample": "Cell culture supernatant", "range": "98 - 100 %", "average": "= 99" }, { "sample": "Urine", "range": "88 - 91 %", "average": "= 89" }, { "sample": "Milk", "range": "100 - 103 %", "average": "= 101" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab322364-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB322364-CMP10" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB322364-CMP08" }, { "size":"20 x 1 Vial", "name":"Human ENPP2/ATX Lyophilized Recombinant Protein", "number":"AB322364-CMP03" }, { "size":"10 x 600 µL", "name":"10X Human ENPP2/ATX Detector Antibody", "number":"AB322364-CMP02" }, { "size":"10 x 1 Vial", "name":"Lyophilized Human ENPP2/ATX Capture Antibody", "number":"AB322364-CMP01" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB322364-CMP06" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB322364-CMP07" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322364-CMP05" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB322364-CMP04" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB322364-CMP09" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab322364-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB322364-CMP10" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB322364-CMP08" }, { "size":"2 x 1 Vial", "name":"Human ENPP2/ATX Lyophilized Recombinant Protein", "number":"AB322364-CMP03" }, { "size":"1 x 600 µL", "name":"10X Human ENPP2/ATX Detector Antibody", "number":"AB322364-CMP02" }, { "size":"1 x 1 Vial", "name":"Lyophilized Human ENPP2/ATX Capture Antibody", "number":"AB322364-CMP01" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB322364-CMP06" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB322364-CMP07" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB322364-CMP11" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB322364-CMP04" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322364-CMP05" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab322364-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB322364-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human ENPP2/ATX Lyophilized Recombinant Protein", "number":"AB322364-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ENPP2/ATX Detector Antibody", "number":"AB322364-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized Human ENPP2/ATX Capture Antibody", "number":"AB322364-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB322364-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB322364-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB322364-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB322364-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB322364-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB322364-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Secreted lysophospholipase D that hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed : 12354767, PubMed : 14500380, PubMed : 15769751, PubMed : 26371182, PubMed : 27754931). Its major substrate is lysophosphatidylcholine (PubMed : 12176993, PubMed : 14500380, PubMed : 27754931). Can also act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility (PubMed : 14500380). Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP (PubMed : 12176993, PubMed : 15769751). Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation (PubMed : 11559573). Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein (PubMed : 1733949). May have a role in induction of parturition (PubMed : 12176993). Possible involvement in cell proliferation and adipose tissue development (Probable). Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18 : 2 and 20 : 4 fatty acids (PubMed : 21393252). Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) (PubMed : 21393252).

See full target information ENPP2

Alternative Names

ATX, PDNP2, ENPP2, Autotaxin, Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, Extracellular lysophospholipase D, E-NPP 2, LysoPLD

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human ENPP2/ATX ELISA Kit

Sandwich ELISA - Human ENPP2/ATX ELISA Kit (AB322364)

Sandwich ELISA - Human ENPP2/ATX ELISA Kit (AB322364)

Sandwich ELISA - Human ENPP2/ATX ELISA Kit (AB322364)

Sandwich ELISA - Human ENPP2/ATX ELISA Kit (AB322364)

Sandwich ELISA - Human ENPP2/ATX ELISA Kit (AB322364)

Sandwich ELISA - Human ENPP2/ATX ELISA Kit (AB322364)

Sandwich ELISA - Human ENPP2/ATX ELISA Kit (AB322364)

Sandwich ELISA - Human ENPP2/ATX ELISA Kit (AB322364)

Sandwich ELISA - Human ENPP2/ATX ELISA Kit (AB322364)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Assay Kits

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Alternative Names

Product promise