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AB113338

Human Entactin ELISA Kit (NID1)

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Human Entactin ELISA Kit (NID1) is a Sandwich (quantitative) ELISA for the measurement of Human Entactin (NID1) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

NID, NID1, Nidogen-1, NID-1, Entactin

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Sandwich ELISA - Human Entactin ELISA Kit (NID1) (AB113338)
  • sELISA

Supplier Data

Sandwich ELISA - Human Entactin ELISA Kit (NID1) (AB113338)

Representative Standard Curve using ab113338

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 0.1 ng/mL

Range

0.123 - 30 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Entactin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Entactin in serum, plasma, cell culture supernatants.

This assay employs an antibody specific for Human Entactin coated on a 96-well plate. Standards and samples are pipetted into the wells and Entactin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Entactin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Entactin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "111 - 138 %", "average": "= 125.7" }, { "sample": "Serum", "range": "123 - 145 %", "average": "= 132.3" }, { "sample": "Plasma", "range": "122 - 147 %", "average": "= 131.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab113338-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB113338-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB113338-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB113338-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human Entactin", "number":"AB113338-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB113338-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Entactin Microplate (12 x 8 strips)", "number":"AB113338-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB113338-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human Entactin Standard (lyophilized)", "number":"AB113338-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Entactin also known as nidogen-1 or NID1 is a significant glycoprotein of the extracellular matrix with a molecular mass of approximately 150 kDa. This protein is often referred to as nidogen and nidogen 1. It localizes mainly in basement membranes across various tissues. Entactin serves as an important structural component and mediator between other extracellular molecules contributing significantly to the maintenance and integrity of the basement membrane structure.
Biological function summary

Entactin interacts closely with laminin and type IV collagen within the extracellular matrix forming an important part of the basement membrane complex. This protein aids in the stabilization and linkage of these matrices playing an essential role in cellular adhesion and migration processes. By facilitating the structural assembly of basement membranes entactin supports cellular signaling pathways that are involved in tissue development and repair.

Pathways

Entactin acts within the integrin signaling pathway and the basement membrane formation pathway. It interacts with integrin receptors on the cell surface thereby influencing processes like cell adhesion and morphogenesis. Through these pathways entactin signals in coordination with laminins and collagens building a robust structural framework necessary for tissue integrity and function.

Entactin's disruption has links to cancer and certain genetic disorders affecting basement membrane integrity. Aberrant expression or dysfunction of entactin can contribute to the progression of tumor invasion due to its pivotal role in cellular adhesion. Furthermore it associates with muscular dystrophies through interactions with laminins and collagens highlighting its importance in maintaining normal tissue architecture and function.
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Product protocols

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Target data

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
See full target information NID1
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websiteProtocolBooklet
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