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Human Entactin ELISA Kit (NID1) is a Sandwich (quantitative) ELISA kit for the measurement of Human Entactin (NID1) in Human in Plasma, Cell culture supernatant, Serum samples.

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Images

Sandwich ELISA - Human Entactin ELISA Kit (NID1) (AB113338), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.123 - 30 ng/mL

Sensitivity

< 0.1 ng/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.

Alternative names

What's included?

1 x 96 Tests
Components
200X HRP-Streptavidin Concentrate
1 x 200 µL
20X Wash Buffer
1 x 25 mL
5X Assay Diluent
1 x 15 mL
Biotinylated anti-Human Entactin
2 x 1 Vial
Entactin Microplate (12 x 8 strips)
1 x 1 Unit
Recombinant Human Entactin Standard (lyophilized)
2 x 1 Vial
Stop Solution
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Human Entactin ELISA Kit (NID1) is a Sandwich (quantitative) ELISA kit for the measurement of Human Entactin (NID1) in Human in Plasma, Cell culture supernatant, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.123 - 30 ng/mL

Assay Platform

Microplate

Sensitivity

< 0.1 ng/mL

General recovery

> 100%

Recovery

Sample specific recovery

Sample type

Cell culture supernatant

Average %

= 125.7

Range

111 - 138 %

Sample type

Serum

Average %

= 132.3

Range

123 - 145 %

Sample type

Plasma

Average %

= 131.4

Range

122 - 147 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Abcam's Entactin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Entactin in serum, plasma, cell culture supernatants.

This assay employs an antibody specific for Human Entactin coated on a 96-well plate. Standards and samples are pipetted into the wells and Entactin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Entactin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Entactin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Entactin also known as nidogen-1 or NID1 is a significant glycoprotein of the extracellular matrix with a molecular mass of approximately 150 kDa. This protein is often referred to as nidogen and nidogen 1. It localizes mainly in basement membranes across various tissues. Entactin serves as an important structural component and mediator between other extracellular molecules contributing significantly to the maintenance and integrity of the basement membrane structure.

Biological function summary

Entactin interacts closely with laminin and type IV collagen within the extracellular matrix forming an important part of the basement membrane complex. This protein aids in the stabilization and linkage of these matrices playing an essential role in cellular adhesion and migration processes. By facilitating the structural assembly of basement membranes entactin supports cellular signaling pathways that are involved in tissue development and repair.

Pathways

Entactin acts within the integrin signaling pathway and the basement membrane formation pathway. It interacts with integrin receptors on the cell surface thereby influencing processes like cell adhesion and morphogenesis. Through these pathways entactin signals in coordination with laminins and collagens building a robust structural framework necessary for tissue integrity and function.

Associated diseases and disorders

Entactin's disruption has links to cancer and certain genetic disorders affecting basement membrane integrity. Aberrant expression or dysfunction of entactin can contribute to the progression of tumor invasion due to its pivotal role in cellular adhesion. Furthermore it associates with muscular dystrophies through interactions with laminins and collagens highlighting its importance in maintaining normal tissue architecture and function.

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1 product image

  • Sandwich ELISA - Human Entactin ELISA Kit (NID1) (ab113338), expandable thumbnail

    Sandwich ELISA - Human Entactin ELISA Kit (NID1) (ab113338)

    Representative Standard Curve using ab113338

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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