JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB108734

Human Anti-Entamoeba histolytica IgG ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Anti-Entamoeba histolytica IgG ELISA Kit is an indirect ELISA for the qualitative detection of IgG class antibodies against Entamoeba histolytica in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Assay Platform

Microplate

style
left-column

Complementary Products

Assay Kits

AB108769

Human Anti-Schistosoma mansoni IgG ELISA Kit

  • 0
  • 0
Assay Kits

AB108737

Human Anti-Herpes simplex virus Type 1 IgG ELISA Kit (HSV1)

  • 0
  • 0
Assay Kits

AB108730

Human Anti-Epstein Barr virus IgG ELISA Kit (EBV-VCA)

  • 5
  • 1
Assay Kits

AB108707

Human Anti-Ascaris lumbricoides IgG ELISA Kit

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's anti-Entamoeba histolytica IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Entamoeba histolytica in Human serum and plasma.

A 96-well plate has been precoated with Entamoeba histolytica antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a Protein A peroxidase labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Entamoeba histolytica-specific antibodies. TMB is then catalyzed by the Protein A to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Entamoeba histolytica IgG sample captured in plate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 7, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.1" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108734-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108734-CMP02", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgG Sample Diluent", "number":"AB108734-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108734-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108734-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108734-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB108734-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Entamoeba histolytica Coated Microplate (12 x 8 wells)", "number":"AB108734-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"Entamoeba histolytica Protein A Conjugate", "number":"AB108734-CMP08", "productcode":"" }, { "size":"1 x 2 mL", "name":"Entamoeba histolytica (IgG) Negative Control", "number":"AB108734-CMP06", "productcode":"" }, { "size":"1 x 3 mL", "name":"Entamoeba histolytica (IgG) Cut-off Control", "number":"AB108734-CMP05", "productcode":"" }, { "size":"1 x 2 mL", "name":"Entamoeba histolytica (IgG) Positive Control", "number":"AB108734-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Entamoeba histolytica IgG an antibody specific to the parasite Entamoeba histolytica plays a significant role in the immune response. Entamoeba histolytica is a protozoan parasite responsible for amoebiasis commonly known as amoebic dysentery. The IgG form of antibodies is an important marker used in the detection and study of this parasite. The molecular mass of Entamoeba histolytica IgG remains consistent with typical IgG antibodies approximately 150 kDa. This antibody gets expressed in response to infection by Entamoeba histolytica a condition that leads to an inflammatory response primarily observed in the intestinal and liver tissues.
Biological function summary

Entamoeba histolytica IgG contributes to immune defense by identifying and marking Entamoeba histolytica cells for destruction by other immune cells. It does not form part of a larger protein complex but instead operates through its specific binding capacity. Upon binding to antigens presented by Entamoeba histolytica this IgG type facilitates the process of phagocytosis by engaging phagocytic cells such as macrophages and neutrophils. This immune response serves as a primary line of defense to control the infection and limit the spread of the parasite within the host's body.

Pathways

Entamoeba histolytica IgG gets engaged in the humoral immune response which is integral to the adaptive immune system. This process involves pathways like the complement system pathway wherein the binding of IgG to the parasite antigens activates a cascade that leads to opsonization complement fixation and eventual lysis of the parasitic cells. Additionally the antibody engages in antibody-dependent cellular cytotoxicity (ADCC) pathways which involve recruiting natural killer cells to target the infected cells thereby reducing parasite survival and proliferation.

Entamoeba histolytica IgG is closely linked to intestinal amoebiasis and amoebic liver abscess. The presence of this IgG in blood serology tests helps diagnose these conditions early. During the pathogenesis of these diseases Entamoeba histolytica interacts with other host proteins like complement proteins and those involved in the liver's inflammatory response such as cytokines like TNF-alpha and IL-1beta. Increased levels of Entamoeba histolytica IgG suggest ongoing infection or exposure to the pathogen aiding clinicians in disease management.
style
left-column

Product protocols

style
left-column

Target data

style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com