AB226898

Human Eotaxin-2 ELISA Kit

Recombinant
SimpleStep
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Human Eotaxin-2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Eotaxin-2 with a sensitivity of 15.35 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

MPIF2, SCYA24, CCL24, C-C motif chemokine 24, CK-beta-6, Eosinophil chemotactic protein 2, Myeloid progenitor inhibitory factor 2, Small-inducible cytokine A24, Eotaxin-2, MPIF-2

5 Images

Sandwich ELISA - Human Eotaxin-2 ELISA Kit (AB226898)

sELISA

Supplier Data

Sandwich ELISA - Human Eotaxin-2 ELISA Kit (AB226898)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Eotaxin-2 concentration was determined to be 13,607 pg/mL with a range of 4,760 – 35,609 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Eotaxin-2 ELISA Kit (AB226898)

Interpolated concentrations of native Eotaxin-2 in human PBMC cell culture supernatant.

PBMCs were cultured in RPMI media and either stimulated with 1.5% PHA or unstimulated for 48 hours. The concentrations of Eotaxin-2 were measured in duplicate and interpolated from the Eotaxin-2 standard curve and corrected for sample dilution. Undiluted samples are as follows : PBMC stimulated 3.12% and PBMC unstimulated 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Eotaxin-2 concentration was determined to be 107,776 pg/mL in stimulated PBMCs and 677 pg/mL in unstimulated PBMCs.

sELISA

Unknown

Sandwich ELISA - Human Eotaxin-2 ELISA Kit (AB226898)

Human Eotaxin-2 Competitor standard curve comparison.

Standard curve comparison between Human Eotaxin-2 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human Eotaxin-2 ELISA Kit (AB226898)

Interpolated concentrations of native Eotaxin-2 in human serum and plasma samples.

The concentrations of Eotaxin-2 were measured in duplicates, interpolated from the Eotaxin-2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 3.12%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Eotaxin-2 concentration was determined to be 13,298 pg/mL in serum, 10,364 pg/mL in plasma (citrate), 9,354 pg/mL in plasma (EDTA), and 8,191 pg/mL in plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human Eotaxin-2 ELISA Kit (AB226898)

Example of human Eotaxin-2 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 15.35 pg/mL

Range

78.13 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Eotaxin-2 ELISA Kit (ab226898) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Eotaxin-2 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Eotaxin-2 with 15.35 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Eotaxin-2, also known as CCL24, MPIF-2 or Ckß-6, is a C-C chemokine originally identified in activated monocytes. Eotaxin-2 is expressed in activated T lymphocytes, GM-CSF treated macrophages and dermal fibroblasts. Secretion of Eotaxin-2 is known to recruit and activate CCR3-bearing cells particularly eosinophils as well as basophils. Activation of eosinophils generates reactive oxygen species, lipid mediators of inflammation and degranulation of toxic granule proteins. Chemotaxis and activation of basophils, previously primed with IL-3, lead to release of histamine and leukotriene C4.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.95" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.5" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "102 - 110 %", "average": "= 106" }, { "sample": "Serum", "range": "97 - 105 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "94 - 107 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "78 - 82 %", "average": "= 80" }, { "sample": "Citrate plasma", "range": "99 - 105 %", "average": "= 102" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Eotaxin 2 also known as CCL24 or eotaxin-2 is a chemokine protein with a mass of approximately 10 kDa. It mainly functions as a signaling molecule that recruits specific immune cells to sites of inflammation or tissue damage. This protein is expressed in various tissues with higher levels in the heart lung liver and small intestine. Eotaxin-2 is a member of the CC chemokine family and its expression is often regulated by inflammatory cytokines.

Biological function summary

Eotaxin 2 plays a critical role in the immune response by attracting eosinophils and other immune cells to inflamed or damaged tissues. This attraction is mediated through its interaction with the CCR3 receptor on the surface of target cells. Eotaxin-2 is not part of a larger complex but instead acts as a standalone molecule within the chemokine signaling network. This protein assists in the body's defense by guiding the movement of cells to stimulate immune activity where needed.

Pathways

Eotaxin 2 is involved in the chemokine-mediated signaling pathway that directs the migration of immune cells. Another important pathway associated with eotaxin 2 is the inflammatory response pathway. The coordinated interaction with other chemokines such as eotaxin (CCL11) and MCP-4 (CCL13) is essential to its role in inflammation and healing. These interactions facilitate proper cellular responses to various physiological challenges.

Eotaxin 2 has been implicated in asthma and allergic rhinitis conditions characterized by an overactive immune response. In these disorders its production and release contribute to excessive eosinophil accumulation which can exacerbate symptoms. Eotaxin 2 connects to these diseases primarily through its action on the CCR3 receptor and its interaction with related chemokines such as eotaxin (CCL11) which also play a role in promoting eosinophil recruitment and activation.

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Product protocols

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Target data

Chemotactic for resting T-lymphocytes, and eosinophils (PubMed : 9104803, PubMed : 9365122). Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes (PubMed : 9104803, PubMed : 9365122). Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line (PubMed : 9104803, PubMed : 9365122). Binds to CCR3 (PubMed : 9104803, PubMed : 9365122).

See full target information CCL24

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Eotaxin-2 ELISA Kit

Sandwich ELISA - Human Eotaxin-2 ELISA Kit (AB226898)

Sandwich ELISA - Human Eotaxin-2 ELISA Kit (AB226898)

Sandwich ELISA - Human Eotaxin-2 ELISA Kit (AB226898)

Sandwich ELISA - Human Eotaxin-2 ELISA Kit (AB226898)

Sandwich ELISA - Human Eotaxin-2 ELISA Kit (AB226898)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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