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AB100509

Human Eotaxin 2 ELISA Kit (CCL24)

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(3 Publications)

Human Eotaxin 2 ELISA Kit (CCL24) is a Sandwich (quantitative) ELISA for the measurement of Human Eotaxin 2 (CCL24) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

MPIF2, SCYA24, CCL24, C-C motif chemokine 24, CK-beta-6, Eosinophil chemotactic protein 2, Myeloid progenitor inhibitory factor 2, Small-inducible cytokine A24, Eotaxin-2, MPIF-2

2 Images
Sandwich ELISA - Human Eotaxin 2 ELISA Kit (CCL24) (AB100509)
  • sELISA

Supplier Data

Sandwich ELISA - Human Eotaxin 2 ELISA Kit (CCL24) (AB100509)

Representative Standard Curve using ab100509.

Sandwich ELISA - Human Eotaxin 2 ELISA Kit (CCL24) (AB100509)
  • sELISA

Supplier Data

Sandwich ELISA - Human Eotaxin 2 ELISA Kit (CCL24) (AB100509)

Representative Standard Curve using ab100509.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 2 pg/mL

Range

1.37 - 1000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Eotaxin 2 (CCL24) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Eotaxin 2 in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human Eotaxin 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Eotaxin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Eotaxin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Eotaxin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get results in 90 minutes with Human Eotaxin-2 ELISA Kit (CCL24) (ab179882) from our SimpleStep ELISA® range.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "82 - 103 %", "average": "= 95.77" }, { "sample": "Serum", "range": "83 - 103 %", "average": "= 94.86" }, { "sample": "Plasma", "range": "85 - 104 %", "average": "= 96.74" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100509-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Recombinant Human Eotaxin 2 Standard (lyophilized)", "number":"AB100509-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB100509-CMP02", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100509-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100509-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100509-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Eotaxin 2 Microplate (12 x 8 well strips)", "number":"AB100509-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human Eotaxin 2", "number":"AB100509-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100509-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100509-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Eotaxin 2 also known as CCL24 or eotaxin-2 is a chemokine protein with a mass of approximately 10 kDa. It mainly functions as a signaling molecule that recruits specific immune cells to sites of inflammation or tissue damage. This protein is expressed in various tissues with higher levels in the heart lung liver and small intestine. Eotaxin-2 is a member of the CC chemokine family and its expression is often regulated by inflammatory cytokines.
Biological function summary

Eotaxin 2 plays a critical role in the immune response by attracting eosinophils and other immune cells to inflamed or damaged tissues. This attraction is mediated through its interaction with the CCR3 receptor on the surface of target cells. Eotaxin-2 is not part of a larger complex but instead acts as a standalone molecule within the chemokine signaling network. This protein assists in the body's defense by guiding the movement of cells to stimulate immune activity where needed.

Pathways

Eotaxin 2 is involved in the chemokine-mediated signaling pathway that directs the migration of immune cells. Another important pathway associated with eotaxin 2 is the inflammatory response pathway. The coordinated interaction with other chemokines such as eotaxin (CCL11) and MCP-4 (CCL13) is essential to its role in inflammation and healing. These interactions facilitate proper cellular responses to various physiological challenges.

Eotaxin 2 has been implicated in asthma and allergic rhinitis conditions characterized by an overactive immune response. In these disorders its production and release contribute to excessive eosinophil accumulation which can exacerbate symptoms. Eotaxin 2 connects to these diseases primarily through its action on the CCR3 receptor and its interaction with related chemokines such as eotaxin (CCL11) which also play a role in promoting eosinophil recruitment and activation.
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Product protocols

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Target data

Chemotactic for resting T-lymphocytes, and eosinophils (PubMed : 9104803, PubMed : 9365122). Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes (PubMed : 9104803, PubMed : 9365122). Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line (PubMed : 9104803, PubMed : 9365122). Binds to CCR3 (PubMed : 9104803, PubMed : 9365122).
See full target information CCL24
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 8: PubMed37345655

2023

CCL24 regulates biliary inflammation and fibrosis in primary sclerosing cholangitis.

Applications

Unspecified application

Species

Unspecified reactive species

Raanan Greenman,Michal Segal-Salto,Neta Barashi,Ophir Hay,Avi Katav,Omer Levi,Ilan Vaknin,Revital Aricha,Sarit Aharoni,Tom Snir,Inbal Mishalian,Devorah Olam,Johnny Amer,Ahmad Salhab,Rifaat Safadi,Yaakov Maor,Palak Trivedi,Christopher J Weston,Francesca Saffioti,Andrew Hall,Massimo Pinzani,Douglas Thorburn,Amnon Peled,Adi Mor

Immunity, inflammation and disease 10:e692 PubMed36301023

2022

HDAC4 depletion ameliorates IL-13-triggered inflammatory response and mucus production in nasal epithelial cells via activation of SIRT1/NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hangyu Xu,Lingjun Wang,Huaqun Chen,Hefei Cai

JHEP reports : innovation in hepatology 2:100064 PubMed32039405

2020

A blocking monoclonal antibody to CCL24 alleviates liver fibrosis and inflammation in experimental models of liver damage.

Applications

Unspecified application

Species

Unspecified reactive species

Michal Segal-Salto,Neta Barashi,Avi Katav,Vicktoria Edelshtein,Arnon Aharon,Sharon Hashmueli,Jacob George,Yaakov Maor,Massimo Pinzani,Dan Haberman,Andrew Hall,Scott Friedman,Adi Mor
View all publications
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