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Human Eotaxin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Eotaxin in Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant samples.

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Images

Sandwich ELISA - Human Eotaxin ELISA Kit (AB318938), expandable thumbnail
  • Sandwich ELISA - Human Eotaxin ELISA Kit (AB318938), expandable thumbnail
  • Sandwich ELISA - Human Eotaxin ELISA Kit (AB318938), expandable thumbnail

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

101.563 - 6500 pg/mL

Assay time

1h 30m

Sensitivity

= 46.692 pg/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info
-
Notes

-

Target data

Function

In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions (PubMed:8597956). Binds to CCR3 (PubMed:8631813).

Alternative names

What's included?

1 x 384 Tests
Components
10X Human Eotaxin Capture Antibody
1 x 600 µL
10X Human Eotaxin Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
384 well CaptSure™ microplates
1 x 384 Unit
Antibody Diluent 4BI
1 x 6 mL
Human Eotaxin Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seal
1 x 1 Unit
Sample Diluent 75BP
1 x 20 mL
Stop Solution
2 x 12 mL
TMB Development Solution
2 x 12 mL

Recommended products

Human Eotaxin ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Eotaxin in Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant samples.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

101.563 - 6500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 46.692 pg/mL

General recovery

107%

Precision

Intra assay

Sample

Serum

n
8
mean
-
SD
-
C.V.

6.5

Inter assay

Sample

Serum

n
3
mean
-
SD
-
C.V.

4.4

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 107

Range

100 - 114 %

Sample type

Citrate plasma

Average %

= 103

Range

96 - 111 %

Sample type

EDTA Plasma

Average %

= 105

Range

101 - 111 %

Sample type

Cell culture supernatant

Average %

= 112

Range

105 - 115 %

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Human Eotaxin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Eotaxin protein in Serum, Cit Plasma, EDTA Plasma, Cell culture supernatant. Quantitate Human Eotaxin with 46.692 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Eotaxin also known as CCL11 or eotaxin-1 is a chemokine belonging to the CC chemokine family. This protein has a molecular mass of approximately 8.4 kDa. Eotaxin is mainly expressed in epithelial cells fibroblasts and smooth muscle cells. High levels of expression occur during inflammatory processes where it acts as a chemoattractant guiding eosinophils to sites of inflammation.

Biological function summary

Eotaxin plays an important role in immune response regulation particularly in allergic reactions. It functions by binding to CCR3 a receptor found on eosinophils therefore facilitating their migration. This process includes involvement in the formation of protein complexes that modulate cell signaling. Eotaxin's primary biological role lies in promoting the accumulation of eosinophils which are important for the immune defense against parasitic infections.

Pathways

Eotaxin participates in the MAPK/ERK signaling pathway which influences cell migration and proliferation. Additionally it operates within the NF-kB pathway often regulating inflammation and immune response. In these pathways eotaxin interacts with other proteins like CCR3 and IL-5 contributing to a coordinated response in immune signaling.

Associated diseases and disorders

Eotaxin is closely linked to allergic asthma and atopic dermatitis. In both these conditions eotaxin-mediated eosinophil recruitment and activation worsen inflammation. Research shows significant eotaxin expression in asthmatic airway tissues. It also connects with proteins like IL-5 which amplify eosinophilic activity leading to exacerbated allergic responses.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Human Eotaxin ELISA Kit (ab318938), expandable thumbnail

    Sandwich ELISA - Human Eotaxin ELISA Kit (ab318938)

    Example of human Eotaxin standard curve in Sample Diluent 75BP.
    Example of human Eotaxin standard curve. Background-subtracted data values (mean +/- SD) are graphed. 

  • Sandwich ELISA - Human Eotaxin ELISA Kit (ab318938), expandable thumbnail

    Sandwich ELISA - Human Eotaxin ELISA Kit (ab318938)

    Interpolated concentrations of Eotaxin in normal human serum donors.
    Serum of eight individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Eotaxin concentration of detected samples was determined to be 104.2 pg/mL with a range of undetected – 183.6 pg/mL.  

  • Sandwich ELISA - Human Eotaxin ELISA Kit (ab318938), expandable thumbnail

    Sandwich ELISA - Human Eotaxin ELISA Kit (ab318938)

    Example of human Eotaxin standard curve in Sample Diluent 75BP in 96-well vs. 384-well plate.
    Example of human Eotaxin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed. 

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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