Human EpCAM ELISA Kit is a single-wash 90-min Simplestep used to quantify Human EpCAM with a sensitivity of 30 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- BMC cancer 21:3152021Detection of tumor-derived extracellular vesicles in plasma from patients with solid cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSilvia R Vitale,Jean A Helmijr,Marjolein Gerritsen,Hicret Coban,Lisanne F van Dessel,Nick Beije,Michelle van der Vlugt-Daane,Paolo Vigneri,Anieta M Sieuwerts,Natasja Dits,Martin E van Royen,Guido Jenster,Stefan Sleijfer,Martijn Lolkema,John W M Martens,Maurice P H M JansenPubMed 33761899
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Cell culture media, Cell culture supernatant, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 78.11 - 5000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 30 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Alternative names
CD326, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, EPCAM, Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, EGP, EGP314, hEGP314
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Human EpCAM ELISA Kit is a single-wash 90-min Simplestep used to quantify Human EpCAM with a sensitivity of 30 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Cell culture media, Cell culture supernatant, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 78.11 - 5000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 30 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Supernatant | n 8 | C.V. 6.1 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Supernatant | n 2 | C.V. 1.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 116 | Range 101 - 126 % |
Sample type Urine | Average % = 109 | Range 101 - 120 % |
Sample type Cell culture media | Average % = 117 | Range 113 - 125 % |
Sample type Cell Lysate | Average % = 106 | Range 96 - 117 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human EpCAM ELISA Kit (ab264632) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of EpCAM protein in cell culture supernatant, cell lysate, urine, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human EpCAM with 30 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Epithelial cell adhesion molecule (EpCAM) is a single-pass type I plasma membrane glycoprotein that may act as a physical homophilic interaction molecule between intestinal epithelial cells and intraepithelial lymphocytes at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. EpCAM plays a role in embryonic stem cells proliferation and differentiation.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.
- Biological function summary
EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.
- Pathways
EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.
- Associated diseases and disorders
EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.
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3 product images
Sandwich ELISA - Human EpCAM ELISA Kit (ab264632)
Example of human EpCAM standard curve in Sample Diluent NSE.
The EpCAM standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human EpCAM ELISA Kit (ab264632)
Interpolated concentrations of native EpCAM in human HT-29 cell culture supernatant and urine samples.
The concentrations of EpCAM were measured in duplicates, interpolated from the EpCAM standard curves and corrected for sample dilution. Undiluted samples are as follows: HT-29 cell culture supernatant 75%, urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EpCAM concentration was determined to be 5,467 pg/mL in neat HT-29 cell culture supernatant and 947 pg/mL in neat urine.
Sandwich ELISA - Human EpCAM ELISA Kit (ab264632)
Interpolated concentrations of native EpCAM in human HT-29 cell based on a 25 μg/mL extract load, human HCT-116 cell based on a 4 µg/mL extract load and colon tissue based on a 400 µg/mL extract load.
The concentrations of EpCAM were measured in duplicate and interpolated from the EpCAM standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean EpCAM concentration was determined to be 4,578 pg/mL in HT-29 cell extract, 2,825 pg/mL in HCT-116 cell extract and 2,391 pg/mL in colon extract.
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